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MICRO-CONTROLE / Spectra-Physics
High Performance Filters for Fluorescence Microscopy

High Performance Filters for Fluorescence Microscopy

Product catalog summary

Introduction
Newport has launched a series of high-performance filters for fluorescence microscopy, previously exclusive to OEM applications, now available as standard catalog products. These filters cater to diverse fluorescence needs, with a fluorophore reference table and spectral performance curves for easy reference.

Product Features
Newport's Corion® Filters, enhanced by Stabilife® technology, provide high transmission, steep transitions, and stable performance under various conditions. They are suitable for applications like cytometry, DNA sequencing, and in-vivo imaging, available in quantities from one to a million.

HPF™ Filters for Microscopy
The HPF™ series includes filters for popular and new fluorophores, designed for epi-fluorescence microscopy. Features include high passband transmission, extended range blocking, and optimized optical finishing. Specifications include ≥90% passband transmittance and ≥OD6 spectral blocking.

Specifications
Excitation, dichroic, and emission filters have specific transmittance, reflectance, and blocking specifications. Coating specifications include hardness, abrasion resistance, adhesion, and humidity resistance, with operating temperatures from -100 ºC to 300 ºC.

Filter Set Selection
Newport offers a comprehensive list of HPF™ fluorescence filter sets tailored for specific fluorophores, with a numerical reference table and spectral data curves to aid selection.

Conclusion
Newport's high-performance filters provide reliable solutions for fluorescence microscopy, supported by extensive experience and patented technology.

Overview
The document details high-performance fluorescence filters for microscopy, focusing on spectral data, stability, and durability, highlighting Stabilife® coating technology for enhanced performance.

Filter Specifications
Each filter set includes an excitation filter, a dichroic filter, and an emission filter, with specific numbers and compatible fluorophores listed.

Stabilife® Coating Technology
This technology uses Reactive Ion Plating (RIP) and Hybrid Plasma Enhanced Deposition (HPED) for dense, durable thin-film coatings, offering high spectral stability.

Humidity and Thermal Stability
Stabilife® filters show minimal spectral shifts under varying humidity and temperature, suitable for demanding applications like fluorescence detection.

Durability
Stabilife® coatings resist damage from handling, radiation, and environmental conditions, meeting military specifications for adhesion, abrasion resistance, and humidity resistance.

Applications
The filters are used in clinical chemistry systems, DNA analysis, laser safety eyewear, and environmental monitoring systems.

Product Range
Newport manufactures various optical products, including anti-reflection, bandpass, beamsplitter, dichroic, and fluorescence filters, covering a spectral range from 150 nm to 16 µm.

Ordering Information
Filters can be ordered as complete sets or customized in size, with contact information for sales inquiries provided.

Manufacturing and Inspection
Newport processes orders from single units to one million, maintaining high customer satisfaction, with coating chambers for various substrates and high precision spectral measurements.

Precision Optics
Located in Irvine, CA, Newport's precision optics manufacturing offers comprehensive fabrication for plano, spherical, and aspherical optics, using materials like fused silica and II-IV materials.

Trademark Information
Trademarks such as Alexa Fluor®, BODIPY®, and others are associated with Molecular Probes, Inc., GE Healthcare, Ltd., and Newport Corporation.

Certifications and Contact Information
Newport Corporation is ISO 9001 certified by the British Standards Institution, with contact details for international offices provided.

Catalog excerpts

High Performance Filters for Fluorescence Microscopy-1

High Performance Filters for Fluorescence Microscopy Solutions to Make, Manage and Measure LightSM Newport. Experience | Solutions

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High Performance Filters for Fluorescence Microscopy-2

High Performance Filters for Fluorescence Microscopy Trust Our Proven History, For Your Fluorescence Filter Needs Newport’s Corion® Filters have been meeting the needs of our customers for over 35 years. Our fluorescence filters are the result of a perfect blend of proven OEM filter manufacturing and our patented Stabilife® technology. This experience and patented process delivers filters with extremely high transmission, steep transitions from blocking to transmission and superior image quality combined with stable performance under changing environmental conditions. In addition, we have tremendous...

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High Performance Filters for Fluorescence Microscopy-3

High Performance Filters for Fluorescence Microscopy • Patented Stabilife® coatings • Shift free spectral response @ 0% - 100%RH • Humidity per MIL-STD-810, Method 507.3, Procedure III, 20 cycles • Lowest autofluorescence • Extremely sharp transitions from rejection to transmission regions • Maximum transmission • Excellent set signal-to-noise ratio • Optimized optical fabrication for brilliant imaging • Superior field life Newport's HPF™ series of filters for fluorescence detection is a compilation of filter product designs developed to be used with many of the most popular standard fluorophores...

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High Performance Filters for Fluorescence Microscopy-4

High Performance Filters for Fluorescence Microscopy Filter Set Selection Newport's HPF™ Fluorescence Filter Sets are listed in the two tables that follow. The Fluorophore Reference table lists the recommended filter set for each listed fluorophore. The Numerical Reference table identifies all of the fluorophores that can be utilized with each filter set. Spectral performance of each filter set is provided for reference in a series of data curves following the tables. HPF™ Fluorescence Filter Sets - Numerical Reference Fluorophore Hoechst 33342 & 33258 Alexa Fluor® 350, AMCA, DAPI LysoSensor™...

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High Performance Filters for Fluorescence Microscopy-5

High Performance Filters for Fluorescence Microscopy HPF™ Fluorescence Filter Sets - Fluorophore Reference Fluorophore 5-FAM 5-ROX 5-TAMRA 6-HEX 6-JOE 6-TET Alexa Fluor® 350 Alexa Fluor® 430 Alexa Fluor® 488 Alexa Fluor® 532 Alexa Fluor® 546 Alexa Fluor® 555 Alexa Fluor® 568 Alexa Fluor® 594 Alexa Fluor® 633 Alexa Fluor® 647 Alexa Fluor® 660 Alexa Fluor® 680 Alexa Fluor® 700 Alexa Fluor® 750 Amca BODIPY® 630/650 BODIPY® 650/665 BODIPY® FL BODIPY® R6G BODIPY® TMR BODIPY® TR Calcium Crimson™ CFP Cy3.5™ Cy3™ Cy5.5™ Cy5™ Cy7™ DAPI Dilfrs) DiO DsRed Eosin Ethidium Bromide FITC Fluo 3 Fluo 4 GFP GFP(rs)...

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High Performance Filters for Fluorescence Microscopy-6

High Performance Filters for Fluorescence Microscopy — Excitation Filter #: HPX 350/60 -Emission Filter #: HPM 460/60 Excitation Filter #: HPX 440/20 Emission Filter #: HPM 480/40 Fluorophores: Hoechst 33342, Hoechst 33258 — Excitation Filter #: HPX 360/60 -Emission Filter #: HPM 460/60 -Excitation Filter #: HPX 450/50 -Emission Filter #: HPM 510/50 Fluorophores: Alexa Fluor® 350, AMCA, DAPI Wavelength (nm) HPF1230 Dichroic Filter #: HPD 520 -Excitation Filter #: HPX 500/20 -Emission Filter #: HPM 530LP Fluorophores: LysoSensor™ Blue (ph5) Fluorophores: Pacific Blue™ Fluorophores: Rhodamine Green™,...

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High Performance Filters for Fluorescence Microscopy-7

High Performance Filters for Fluorescence Microscopy Wavelength (nm) HPF1260 Dichroic Filter #: HPD 515 — Excitation Filter #: HPX 485/30 -Emission Filter #: HPM 545/30 Fluorophores: Oregon Green™ Fluorophores: 5-FAM, Alexa Fluor® 488 -Excitation Filter #: HPX 480/40 —Emission Filter #: HPM 535/50 -Excitation Filter #: HPX 500/40 —Emission Filter #: HPM 560/50 Fluorophores: Eosin Fluorophores: BODIPY® FL, DO, FITC, Fluo 3, Fluo 4, GFP (rs) Fluorophores: Alexa Fluor® 430 Fluorophores: Alexa Fluor® 532 Email: [email protected] • Web: newport.com CV) viewport Experience | Solutions

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High Performance Filters for Fluorescence Microscopy-8

High Performance Filters for Fluorescence Microscopy Fluorophores: Alexa Fluor® 546, Alexa Fluor® 555, TRITC — Excitation Filter #: HPX 510/40 -Emission Filter #: HPM 575/50 -Excitation Filter #: HPX 530/40 —Emission Filter #: HPM 600/60 — Excitation Filter #: HPX 525/45 -Emission Filter #: HPM 585/40 -Excitation Filter #: HPX 535/40 -Emission Filter #: HPM 610/50 -Excitation Filter #: HPX 520/40 -Emission Filter #: HPM 590/55 -Excitation Filter #: HPX 535/50 -Emission Filter #: HPM 620/60 CV) viewport Experience | Solutions

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High Performance Filters for Fluorescence Microscopy-9

High Performance Filters for Fluorescence Microscopy HPF™ Fluorescence Filter Set - Spectral Data (continued) 100 80 60 40 20 Fluorophores: Cy3.5™ Fluorophores: Alexa Fluor® 568, Alexa Fluor® 594 100 HPF1350 -Excitation Filter #: HPX 525/45 Fluorophores: Ethidium Bromide Fluorophores: 5-TAMRA, MitoTracker® Orange Fluorophores: BODIPY® TR Email: [email protected] • Web: newport.com CV) viewport Experience | Solutions

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High Performance Filters for Fluorescence Microscopy-10

High Performance Filters for Fluorescence Microscopy HPF1370 -Excitation Filter #: HPX 525/45 Fluorophores: Propidium Iodide Fluorophores: Cy5™ HPF1375 Excitation Filter #: HPX 560/50 Fluorophores: Resorufin, Texas Red® CV) viewport Experience | Solutions

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High Performance Filters for Fluorescence Microscopy-11

High Performance Filters for Fluorescence Microscopy -Excitation Filter #: HPX 660/40 Emission Filter #: HPM 725/40 Fluorophores: Alexa Fluor® 750 Ordering Information Catalog HPF" filters are available as complete filter sets including an excitation filter, a dichroic filter, and an emission filter. Please consult a sales professional for pricing information. Filter sets may be ordered using their set number. Custom sizes of HPF™ filter sets as well as mounting services are available upon request. Please consult a sales engineer for details. Fluorophores: Alexa Fluor® 680 CV) viewport Experience...

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High Performance Filters for Fluorescence Microscopy-12

High Performance Filters for Fluorescence Microscopy Stabilife® Coating Technology Film Density & Spectral Stability Newport's HPF™ fluorescence filters are manufactured using our patented Stabilife® coating technology. Some of the key features and benefits of this technology are provided in the paragraphs that follow. Film density is a critical factor affecting the spectral stability of an optical coating. Un-stabilized metal oxide thin film coatings typically exhibit a significantly lower packing density than Stabilife coatings. This occurs as a result of the intrinsic growth properties of...

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