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High Performance Filters for Fluorescence Microscopy

High Performance Filters for Fluorescence Microscopy
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High Performance Filters for Fluorescence Microscopy - 1

High Performance Filters for Fluorescence Microscopy Solutions to Make, Manage and Measure LightSM Newport. Experience | Solutions

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High Performance Filters for Fluorescence Microscopy - 2

High Performance Filters for Fluorescence Microscopy Trust Our Proven History, For Your Fluorescence Filter Needs Newport’s Corion® Filters have been meeting the needs of our customers for over 35 years. Our fluorescence filters are the result of a perfect blend of proven OEM filter manufacturing and our patented Stabilife® technology. This experience and patented process delivers filters with extremely high transmission, steep transitions from blocking to transmission and superior image quality combined with stable performance under changing environmental conditions. In addition, we have...

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High Performance Filters for Fluorescence Microscopy - 3

High Performance Filters for Fluorescence Microscopy • Patented Stabilife® coatings • Shift free spectral response @ 0% - 100%RH • Humidity per MIL-STD-810, Method 507.3, Procedure III, 20 cycles • Lowest autofluorescence • Extremely sharp transitions from rejection to transmission regions • Maximum transmission • Excellent set signal-to-noise ratio • Optimized optical fabrication for brilliant imaging • Superior field life Newport's HPF™ series of filters for fluorescence detection is a compilation of filter product designs developed to be used with many of the most popular standard...

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High Performance Filters for Fluorescence Microscopy - 4

High Performance Filters for Fluorescence Microscopy Filter Set Selection Newport's HPF™ Fluorescence Filter Sets are listed in the two tables that follow. The Fluorophore Reference table lists the recommended filter set for each listed fluorophore. The Numerical Reference table identifies all of the fluorophores that can be utilized with each filter set. Spectral performance of each filter set is provided for reference in a series of data curves following the tables. HPF™ Fluorescence Filter Sets - Numerical Reference Fluorophore Hoechst 33342 & 33258 Alexa Fluor® 350, AMCA, DAPI...

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High Performance Filters for Fluorescence Microscopy - 5

High Performance Filters for Fluorescence Microscopy HPF™ Fluorescence Filter Sets - Fluorophore Reference Fluorophore 5-FAM 5-ROX 5-TAMRA 6-HEX 6-JOE 6-TET Alexa Fluor® 350 Alexa Fluor® 430 Alexa Fluor® 488 Alexa Fluor® 532 Alexa Fluor® 546 Alexa Fluor® 555 Alexa Fluor® 568 Alexa Fluor® 594 Alexa Fluor® 633 Alexa Fluor® 647 Alexa Fluor® 660 Alexa Fluor® 680 Alexa Fluor® 700 Alexa Fluor® 750 Amca BODIPY® 630/650 BODIPY® 650/665 BODIPY® FL BODIPY® R6G BODIPY® TMR BODIPY® TR Calcium Crimson™ CFP Cy3.5™ Cy3™ Cy5.5™ Cy5™ Cy7™ DAPI Dilfrs) DiO DsRed Eosin Ethidium Bromide FITC Fluo 3 Fluo 4 GFP...

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High Performance Filters for Fluorescence Microscopy - 6

High Performance Filters for Fluorescence Microscopy — Excitation Filter #: HPX 350/60 -Emission Filter #: HPM 460/60 Excitation Filter #: HPX 440/20 Emission Filter #: HPM 480/40 Fluorophores: Hoechst 33342, Hoechst 33258 — Excitation Filter #: HPX 360/60 -Emission Filter #: HPM 460/60 -Excitation Filter #: HPX 450/50 -Emission Filter #: HPM 510/50 Fluorophores: Alexa Fluor® 350, AMCA, DAPI Wavelength (nm) HPF1230 Dichroic Filter #: HPD 520 -Excitation Filter #: HPX 500/20 -Emission Filter #: HPM 530LP Fluorophores: LysoSensor™ Blue (ph5) Fluorophores: Pacific Blue™ Fluorophores: Rhodamine...

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High Performance Filters for Fluorescence Microscopy - 7

High Performance Filters for Fluorescence Microscopy Wavelength (nm) HPF1260 Dichroic Filter #: HPD 515 — Excitation Filter #: HPX 485/30 -Emission Filter #: HPM 545/30 Fluorophores: Oregon Green™ Fluorophores: 5-FAM, Alexa Fluor® 488 -Excitation Filter #: HPX 480/40 —Emission Filter #: HPM 535/50 -Excitation Filter #: HPX 500/40 —Emission Filter #: HPM 560/50 Fluorophores: Eosin Fluorophores: BODIPY® FL, DO, FITC, Fluo 3, Fluo 4, GFP (rs) Fluorophores: Alexa Fluor® 430 Fluorophores: Alexa Fluor® 532 Email: filters.sales@newport.com • Web: newport.com CV) viewport Experience | Solutions

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High Performance Filters for Fluorescence Microscopy - 8

High Performance Filters for Fluorescence Microscopy Fluorophores: Alexa Fluor® 546, Alexa Fluor® 555, TRITC — Excitation Filter #: HPX 510/40 -Emission Filter #: HPM 575/50 -Excitation Filter #: HPX 530/40 —Emission Filter #: HPM 600/60 — Excitation Filter #: HPX 525/45 -Emission Filter #: HPM 585/40 -Excitation Filter #: HPX 535/40 -Emission Filter #: HPM 610/50 -Excitation Filter #: HPX 520/40 -Emission Filter #: HPM 590/55 -Excitation Filter #: HPX 535/50 -Emission Filter #: HPM 620/60 CV) viewport Experience | Solutions

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High Performance Filters for Fluorescence Microscopy - 9

High Performance Filters for Fluorescence Microscopy HPF™ Fluorescence Filter Set - Spectral Data (continued) 100 80 60 40 20 Fluorophores: Cy3.5™ Fluorophores: Alexa Fluor® 568, Alexa Fluor® 594 100 HPF1350 -Excitation Filter #: HPX 525/45 Fluorophores: Ethidium Bromide Fluorophores: 5-TAMRA, MitoTracker® Orange Fluorophores: BODIPY® TR Email: filters.sales@newport.com • Web: newport.com CV) viewport Experience | Solutions

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High Performance Filters for Fluorescence Microscopy - 10

High Performance Filters for Fluorescence Microscopy HPF1370 -Excitation Filter #: HPX 525/45 Fluorophores: Propidium Iodide Fluorophores: Cy5™ HPF1375 Excitation Filter #: HPX 560/50 Fluorophores: Resorufin, Texas Red® CV) viewport Experience | Solutions

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High Performance Filters for Fluorescence Microscopy - 11

High Performance Filters for Fluorescence Microscopy -Excitation Filter #: HPX 660/40 Emission Filter #: HPM 725/40 Fluorophores: Alexa Fluor® 750 Ordering Information Catalog HPF" filters are available as complete filter sets including an excitation filter, a dichroic filter, and an emission filter. Please consult a sales professional for pricing information. Filter sets may be ordered using their set number. Custom sizes of HPF™ filter sets as well as mounting services are available upon request. Please consult a sales engineer for details. Fluorophores: Alexa Fluor® 680 CV) viewport...

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High Performance Filters for Fluorescence Microscopy - 12

High Performance Filters for Fluorescence Microscopy Stabilife® Coating Technology Film Density & Spectral Stability Newport's HPF™ fluorescence filters are manufactured using our patented Stabilife® coating technology. Some of the key features and benefits of this technology are provided in the paragraphs that follow. Film density is a critical factor affecting the spectral stability of an optical coating. Un-stabilized metal oxide thin film coatings typically exhibit a significantly lower packing density than Stabilife coatings. This occurs as a result of the intrinsic growth properties...

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