Metrohm Inline Sample Preparation Making your ion chromatography more efficient

Crystal clear, clinically pure, no bacteria and no particles: this would be an ideal sample for ion chromatography. The real world is often different. Apart from the ions to be determined, the samples contain matrix components which may be aggressive or lead to precipitation in the system. They can sometimes make analysis difficult, if not impossible. Samples with a high load of matrix components can damage the separation column or lead to blockages in the system. That is why suitable sample preparation is essential for reliable and accurate analysis. In the past, all sample preparation steps...

In modern ion chromatography it is highly recommended to filter all samples prior to injection, in order to avoid particle deposits in the injection valve, in capillary tubes or on separation columns. Such deposits in turn reduces the chromatographic performance and the lifetime of the columns. With Inline Ultrafiltration, Metrohm offers a fast and economical alternative to manual filtration. Inline Ultrafiltration combines sample injection directly with filtration. Because of this unique functional feature, filters with a pore size of 0.2 pm can be used for 100 or more samples. This is ideal...

Metrohm Inline Dialysis 06 Inline Dialysis at a glance • Time- and cost-saving Inline Sample Preparation for analysis of complex samples • Patented «Stopped-Flow Dialysis» • Fully automatic dialysis of samples during sample injection • Increased column lifetime due to elimination of large molecules, oily sample components, proteins, and particles • No time-consuming manual working steps • Low-maintenance requirement (replacement of dialysis membrane every 40–400 samples) • Minimal carryover from sample to sample (<0.2%) • Nearly complete dialysis rates • Possibility of combination with Inline...

Metrohm Logical Inline Dilution One of the oldest sample preparation techniques is the dilution of samples. Nowadays, this step too can be carried out inline using an automated system. A further development is Logical Inline Dilution (Metrohm Inline Dilution Technique «MIDT»). For this, the sample just needs to be placed on the autosampler and the analysis is started. The system automatically calculates the optimum dilution factor and analyzes the sample. Thus your results are always reliable, because they are always within the calibration range. Of course, not only samples, but also standards...

Metrohm intelligent Partial Loop Injection Technique 10 Unlike with Logical Inline Dilution, the dilution factor is not varied when using «MiPT» – Metrohm intelligent Partial Loop Injection Technique. With this technique, the injection volume of an undiluted sample is adjusted between 2 and 200 µL. The method is «intelligent» be cause it automatically calculates the optimum injection volume. As a result, here too it is possible to cover a very wide range of sample concentrations. Furthermore, this technique is almost free of any carryover (<0.001%), enabling sequential analysis in the mg/L and...

Metrohm intelligent Pick-up Injection Technique 12 Metrohm intelligent Pick-up Technique «MiPuT» is the ideal sample injection technique for small sample quan tities of just a few µL. This technique requires only as much sample volume as the amount needed for injection into the ion chromatograph. Users can choose volumes ranging from 4 µL to 60 µL. The benefit of this technique is that samples from a broad range of concentrations can be delivered without the need for any manual dilution. The optimal injection volume is calculated automatically. A system using Pick-up Injection Technique can be...

Metrohm Inline Extraction 14 Extractions make it possible to transfer components from one medium into another. In ion chromatography, water is used as the basis of the mobile phase. Inline Extraction is used to transfer water soluble components from a non-polar organic phase into an aqueous phase. The aqueous phase can then be injected directly after Inline Dialysis and is analyzed subsequently. Thus whole sample series can be processed fully automatically. Inline Extraction at a glance • Time- and cost-saving Inline Sample Preparation for analysis of organic samples • Unique inline technique...

Metrohm Inline Matrix Elimination 16 The determination of anions or cations in polar organic solvents or other aggressive matrices is an analytical challenge. Such samples can damage the column or affect the chromatograms in such a way that evaluation is impossible. This challenge can be met with Inline Matrix Elimination. Inline Matrix Elimination is a technique that is able to separate ionic analytes from the uncharged matrix with out sample preparation cartridges (SPE cartridges). In addition, the counterions of the analytes and of the matrix are removed. In contrast to Inline Dialysis, small...

Metrohm Inline Preconcentration 18 For each analytical method, there are limits regarding the smallest detectable concentration. In ion chromatography, depending on the system used, these limits are between 0.1 µg/L and 100 µg/L. Inline Preconcentration increases measuring sensitivity considerably and opens up concen tration ranges in the ng/L range. For Inline Preconcentration, a preconcentration column is used instead of a sample loop. This column features functional properties very similar to those of separation columns, except that the material has a considerably higher capacity and consists...

Inline Preconcentration with Inline Matrix Elimination 20 Inline Preconcentration and Inline Matrix Elimination can be combined into Metrohm intelligent Preconcentration Technique with Matrix Elimination (MiPCT-ME), a highperformance sample preparation technique. With MiPCTME, low concentrations of analytes present in a nonionic matrix can be determined with a high degree of reproducibility. Once the sample is concentrated in a preconcentration column, the non-ionic components of the sample are flushed with ultrapure water from the preconcentration column. The advantage of this is that the non-ionic...