Live Cell RNA Detection
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Live Cell RNA Detection SmartFlare™ RNA Detection Probes Merck Millipore is a division of 'MERCK

Live cell RNA detection with SmartFlare™ RNA Detection Probes Instead of lysed cells, switch to live cells. And while you’re at it, eliminate sample preparation and transfection steps all together. Live cell RNA detection is now possible, in a single incubation step using inert nanoparticle technology to specifically detect native RNA. And when you’re done, the probes exit the cells allowing you to perform downstream analyses with the same, unperturbed cells. Make the smart change! Live cell imaging of miR-21 RNA in human prostate cancer cells (DU145) using SmartFlare™ RNA Detection Probes...

What’s smart Use a SmartFlare™ probe to analyze gene expression in live cells with no sample prep, cell lysis or toxicity, and you’ll see just how smart a tiny particle can be. L ive Cell Detection See real-time RNA expression in live cells, giving you more physiologically relevant data. E asy Open-platform Detection Detect the fluorescent signals using a platform of your choice: microscope, personal cell analyzer, flow cytometer, cell sorter, etc. N o Toxicity, Leaving Cells Unchanged Non-toxic nanoparticles that enter and exit cells via natural mechanisms, allowing the same live cells to...

What’s simple Eliminate the complexity and uncertainty associated with other RNA detection techniques. SmartFlare™ RNA Detection Probes make discovery effortless. RNA detection often requires laborious sample prep, RNA amplification and detection based on standard curves. SmartFlare™ RNA Detection Probes are endocytosed by live cells using existing cellular endocytosis machinery. Sample prep is unnecessary; simply add SmartFlare™ probes to your cultures, incubate overnight and detect the next day. Over time, the probes will exit the cell, without any adverse effects, allowing for subsequent...

The SmartFlare™ probe’s simple mechanism reveals the RNA content of live cells while keeping cells alive and intact. Enter Probes enter cell via natural endocytosis Probe approaches RNA target. Probe binds target obe t and are releases for detection. In absence of target, probe is quenched by gold and does not uoresce Probes exit cell via exocytosis. The same cell can be reused for other experiments. When target is present, it will bind the capture strand, releasing the reporter strand, which uoresces Figure 2. Enter, bind, fluoresce, exit. SmartFlare™ probes enter the cell using the cell’s...

What’s powerful You can take a peek at your RNA without your cells ever knowing you were there. SmartFlare™ probes enable novel experimental designs that can change how you do science. Imagine the possibilities. Applications using SmartFlare™ Probes • • your SmartFlare™ probe-treated cells Reuse rack changes in RNA dynamically, T over time • cell detection via flow cytometry Live • Sort cell types that are traditionally difficult to isolate, using intracellular RNA markers • multiple RNA targets simultaneously Assess (multiplex) Quantify miRNAs • Assess RNA and protein in the same cellular...

What’s really happening Using live cells means you are looking at native, unamplified RNA, which can reveal meaningful links between gene expression profiles and phenotypes. Choose the way you discover what’s really happening in your cells. Scrambled Control Figure 4. Microscopy shows specificity and dynamic range of EGFR SmartFlare™ probe. Varying levels of EGFR expression (red) shown in MCF7 (low expression) and HeLa (high expression) cells when incubated with an EGFR-specific SmartFlare™ probe. No signal is seen in cells treated with a sequence-scrambled control probe (left column)....

What’s the best detection platform SmartFlare™ RNA Detection Probes are compatible with almost any fluorescence detection system. After a simple overnight incubation, you are ready to analyze your cell’s RNA content on the platform of your choice. Choose from a wide range of fluorescence detection platforms based on your research needs. Microscopy Personalized Cell Analyzer Flow Cytometry Imaging Flow Cytometry Cell Sorter Live Cell Analysis Relative Quantitation Single Cell Analysis Sorting Capabilities 96-well Format

Merck Millipore offers powerful and innovative options for single cell fluorescence detection. Muse™ Cell Analyzer • Intuitive software and touchscreen interface allow for easy operation - no flow expertise necessary Green laser for flow-based detection of Cy3 fluorophore Optimized assays for cell health, cell signalling and immunology Simplified analysis of miR-196 in single cells with SmartFlare™ probes and the Muse™ Cell Analyzer. guava easyCyte™ Benchtop Flow Cytometer • Automated sampling for 96-well plates No sheath fluid—microcapillary system lets you use less sample and less...

What’s the first step Interested in testing SmartFlare™ probes in your system? Start with our controls. We recommend starting with an Uptake and Housekeeping control to see how SmartFlare™ RNA detection probes can help your research. Whether you’re an experienced user or just interested in performing some initial proof of concept experiments, we have the right control for you. Be confident in your results by having the proper SmartFlare™ controls in your experiments. A. Uptake Control Flare always on, does not bind target. B. Scramble Control No are release. C. Housekeeping Control Flare...

Related Products - SmartFlare™ Controls Description Housekeeping Controls Uptake-Cy3 SmartFlare™ RNA Detection Probe Uptake-Cy5 SmartFlare™ RNA Detection Probe Scramble-Cy3 SmartFlare™ RNA Detection Probe Scramble-Cy5 SmartFlare™ RNA Detection Probe miRNA Scramble-Cy3 SmartFlare™ RNA Detection Probe Uptake Controls 18S Hu, Ms, Rt-Cy3 SmartFlare™ RNA Detection Probe 18S Hu, Ms, Rt-Cy5 SmartFlare™ RNA Detection Probe Beta Actin Hu-Cy3 SmartFlare™ RNA Detection Probe Beta Actin Hu-Cy5 SmartFlare™ RNA Detection Probe Cyclophilin A Hu-Cy3 SmartFlare™ RNA Detection Probe Cyclophilin A Hu-Cy5...