Create and control hypercapnia conditions. Life Science - Pharma: Cell Cultures Studies

Product catalog summary

Project Overview
The collaboration between the Institute of Technology in Lausanne (EPFL) and MCQ Instruments focuses on cell culture studies within the life sciences and pharmaceutical sectors. The project is managed by Prof. John McKinney from the School of Life Sciences, Laboratory of Microbiology and Microtechnology (LMIC).

Role of MCQ Instruments
MCQ Instruments is tasked with creating and controlling specific hypercapnia conditions using their Gas Blender 100 Series device and Pro Version software. This setup allows for stable gas flow and efficient experimentation.

EPFL Background
EPFL is a renowned research institute in Lausanne, Switzerland, specializing in natural sciences and engineering. It is ranked among the top universities globally for engineering and technology.

Project Details

Hypercapnia Condition: The project involves creating stressful hypercapnia conditions to study Mycobacterium tuberculosis replication and ribosomal activity using time-lapse microscopy.
Flow Stability: The revolutionary method ensures stable gas flow, even at lower flow ranges, enhancing experimental accuracy.
Time Savings: The Gas Mixer allows for quick calibration point settings, reducing time and effort compared to traditional methods.
Micro Flows: The GB100 Series controls flow across a wide range without cut-off, from 0.1 ml/min to 500 ml/min.
Software Automation: The Pro Version software enables automated experiments, increasing efficiency.

Benefits and Savings
The use of MCQ Instruments' technology reduces the need for multiple mass flow controllers and pre-mixed gas cylinders, saving time and resources while allowing for a greater number of experiments and data collection.

Conclusion
The collaboration with MCQ Instruments provides EPFL with advanced tools for conducting cell culture studies, enhancing research capabilities and efficiency.

Catalog excerpts

Create and control hypercapnia conditions. Life Science - Pharma: Cell Cultures Studies-1

HYPERCAPNIA CONDITION CELL CULTURE DRUGS & PHARMA A SOLID BUSINESS CASE IN COLLABORATION WITH THE INSTITUTE of TECHNOLOGY in LAUSANNE (EPFL) GENERAL INFORMATION ABOUT THE PROJECT Life Science - Pharma: Cell Cultures Studies School of Life Sciences, Laboratory of Microbiology and Microtechnology (LMIC) HEAD OF PROJECT MANAGEMENT: Prof. John McKinney To create and control speciﬁc hypercapnia conditions. MORE INFORMATION ABOUT THE EPFL The École polytechnique fédérale de Lausanne (EPFL) is a research institute and university in Lausanne, Switzerland, that specializes in natural sciences and engineering. EPFL is widely regarded as a world leading university. The QS World University Rankings ranks EPFL 12th in the world across all ﬁelds in their 2017/2018 ranking, whilst Times Higher Education World University Rankings ranks EPFL as the world's 11th best school for Engineering and Technol

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Create and control hypercapnia conditions. Life Science - Pharma: Cell Cultures Studies-2

DESCRIPTION OF THE APPLICATION AND THE TARGET Nonreplicating and metabolically quiescent bacteria are implicated in latent tuberculosis infections and relapses following ‘‘sterilizing’’ chemotherapy. However, evidence linking bacterial dormancy and persistence in vivo is largely inconclusive. Here we measure the single cell dynamics of Mycobacterium tuberculosis replication and ribosomal activity using quantitative time-lapse microscopy and a reporter of ribosomal RNA gene expression. Single-cell dynamics exhibit heterogeneity under standard growth conditions, which is ampliﬁed by stressful conditions...

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