A Decade of Super-Resolution Innovation Working with passion for the global research community: The TCS SP8 STED 3X marks one decade of innovative super-resolution technology by Leica Microsystems - providing quality data and efficient processes for your science. TCS STED Dual color for TCS SP8 STED TCS SP8 TCS STED (CW) and gated STED STED 3X CONNECT WITH US LASER RADIATION VISIBLE AND INVISIBLE - CLASS 4 AVOID EYE OR SKIN EXPOSURE TO DIRECT OR SCAITERED RAOIATION P< 4W 350-1600nm >801 s IEC 60825-1 : 2007 www.leica-microsystems.com/ products/super-resolution Order no.: English 1593003011 • Copyright © by Leica Microsystems CMS GmbH, Mannheim, Germany, 2014. Subject to modifications. LEICA and the Leica Logo are registered trademarks of Leica Microsystems IR GmbH.

Leica TCS SP8 STED 3X Your Next Dimension!

Welcome To Your Next Dimension! The new TCS SP8 STED 3X opens up new dimensions for your research: Discover the possibilities of super-resolution in real 3D.

Pure Physics, More Efficiency The new TCS SP8 STED 3X strengthens your science: ››Tunable and direct super-resolution in x, y and z reveal smallest details ›› Multiple STED lines open up the full spectrum of visible light ›› Gated detection improves resolution and increases live cell capabilities ›› STED WHITE objective has optimal color correction for the full spectrum ›› Auto beam alignment provides stability and reliability ›› Modular concept based on the TCS SP8 allows you to upgrade at any time ›› Smart STED Wizard intuitively controls your experiments ›› Huygens deconvolution gets more...

TCS SP8 STED 3X – Here’s Your Next Dimension! Here’s Your Next Dimension! STED microscopy by Leica Microsystems has revolutionized the study of subcellular a ­ rchitecture and cell dynamics at the nanoscale. The fully integrated STED (STimulated Emission Depletion) system meets the requirements of daily research and provides fast, intuitive, and purely optical access to structural details far beyond the diffraction limit. Gated STED substantially improves resolution to below 50 nm and increases live cell viability. Now, the next generation of STED, the TCS SP8 STED 3X, broadens the scope of super-resolution...

Pure Physics, More Reliability STimulated Emission Depletion imaging reaches lateral resolution below 50 nm in true optical sections by downscaling the spot from where fluorescence is generated. STED is pure physics: What you see is what you get. The principle Super-resolution – fast and direct The basic principle of STED, which was A phase mask filter determines the area STED technology provides fast and direct first described by Stefan Hell in 1994 , is in the focus plane where STED light is access to structural details at the nano- simple. The effective focal spot scanning dominant, e.g. resulting...

TCS SP8 STED 3X – Pure Physics, More Reliability STED Intensity Achieved resolution (left) correlates with STED laser power (right). Right panel: STED donut with area of stimulated emission in red, area of fluorescence in green. Resolution improvement achieved by STED microscopy is purely optical and does not rely on mathematics. Of course, you can further process your images with deconvolution. STED allows you to directly compare the outcome with the raw data and makes your results more reliable. Don’t waste time with artifacts! Key Publications ›› Hell, S. W. & Wichmann, J. Breaking the diffraction...

Life Happens in 3D – Now Observe its Detail Immunofluorescence stain of Golgi marker in HeLa cells: Surface rendered 3D reconstruction after Huygens deconvolution of a z stack (20 planes), 3D donut 100%. STED data (green) show a clear resolution increase compared to the confocal result (red). Data courtesy of Dr. Timo Zimmermann, Center for Genomic Regulation, Barcelona, Spain.

Histone H3-Alexa 568 in HeLa cells: The highest lateral resolution increase is achieved by the vortex donut, maximal resolution increase in x, y and z by 3D STED. Note the loss of structures of objects that are not in the super-resolved focus plane when using 3D STED. 3D STED also achieves an increase of resolution in the xy dimension. 11#ii itx axisz axis0% Z Donut60% Z Donut XZ axis plot of Histone H3-Alexa 568 in HeLa cells. 0% z donut allocates all STED light to the vortex donut resulting in maximal resolution increase in x and y, but not in z compared to confocal microscopy. A small focal...

Push the Boundaries! Organisms, tissues and cells are three dimensional. To investigate cellular processes you want to consider all directions. The new TCS SP8 STED 3X allows you to extend the boundaries of super-resolution in all dimensions. Discover more details in X, Y and Z Engineer your PSF to your science With TCS SP8 STED 3X, two STED light paths generate TCS SP8 STED 3X gives you the possibility to match the different STED patterns (see figure below). For best resolution resolution of your microscope in all dimensions to your scientific in x and y the light is allocated to the STED pathway,...

Immunostaining of endosomal marker (Lamp M) in TCS SP8 STED 3X – Subchapter 13 HeLa cells. Note the loss of structures out of superresolved focal plane (white arrows) in the middle compared to confocal images. Courtesy of Shem Johnson, University of Geneva, Switzerland. gSTED with maximum z resolution Push the limits even further The powerful Huygens STED deconvolution package, exclusively supplied with every TCS SP8 STED 3X system, helps to improve your data. Huygens decreases noise levels dramatically and also enhances resolution in x, y and z. After deconvolution of STED data, even smaller...

See the Full Spectrum of Life Seeing subcellular structures in nanoscopic detail opens a new world to scientists. Multicolor applications give access to detailed information about the interrelationships of various structures. The new STED 3X module offers multiple STED laser lines at different wavelengths in one instrument. Super-resolved colocalization studies have never been so easy. Tunable spectral super-resolution – the freedom to choose Super-resolution and standard labeling strategies are not mutually exclusive. A broad variety of popular dyes and fluorescent proteins in the green spectrum...