TCS SP8 HyD
24Pages

Catalog excerpts

Living up to Life Leica HyD for Confocal Imaging All-Purpose Super-Sensitivity

Reduce light dosage, increase cell viability Excitation 488 Emission 490-556 nm Scan frequency 400 Hz No accumulation No averaging

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Leica HyD -Your Road to Super-Sensitivity Innovation is a driving force for discovery. The Leica HyD hybrid detector sets a new standard in super-sensitive imaging. There is no need to compromise: photon counting or imaging? Low-light or bright fluorescence? High speed or crisp images? With the Leica HyD you can do it all. GO LIVE WITH HIGH DEFINITION Imaging live cell dynamics presents a challenge for point scanning confocals. To scan fast enough to capture rapid cellular processes typicallly is a tradeoff of sensitivity to gain speed of image capture. This results in lower signal level...

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Neuromuscular junction in Drosophila melanogaster Three- to four-cell stage of Caenorhabditis elegans labeled with Bruchpilot::mStrawberry. The back- ground of the PMT image is blurred by residual noise amplified by the maximum projection, while the HyD image is devoid of noise. LOW DARK NOISE IMPROVES Z-STACKS HIGH SPEED MAKES C. ELEGANS EMBRYOGENESIS ACCESSIBLE Sensitive live specimens need to be Developmental processes intrinsically imaged under low-light conditions in high involve the time dimension. In order to gain situations. Low noise can become unravel the spatio-temporal formation...

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Live yeast cells double-labeled with EGFP at both glass surface. the nuclear envelope and the telomere. HIGH SENSITIVITY FOR SINGLE INCREASED CELL VIABILITY PERMITS YEAST IMAGING WITH A CONFOCAL POINT SCANNER Fixed single molecules represent High sensitivity directly translates into the ultimate imaging frontier. When reduced light delivered to the specimen measuring such weak signals close to and less bleaching. The Leica HyD detects a reflective surface, sensitivity, dark even delicate systems such as yeast at full noise and the efficiency of the beam confocal resolution. splitting system...

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C. elegans embryos labeled with EGFP-tubulin imaged using either PMT (top row) or HyD (bottom row) with identical instrument parameters. The insets (right column) reveal how the Leica HyD captures the delicate microtubules radiating from the aster. Courtesy of Prof. Pierre Gonczy, EPFL, Lausanne, Switzerland. Leica HyD strongly improves contrast in comparison to PMTs. Sample: Tubulin Excitation: Argon laser 488 nm, 0.2% total power Imaging: 16x accumulation Detection: 500-620 nm PMT Voltage: 800 V HyD gain: 100% The contrast ratio was plotted as the ratio of mean intensity in the darkest...

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Hybrid Detection Technology for High Fidelity With its unparalleled contrast the Leica HyD delivers publication-ready images without post-processing. All imaging tasks benefit from the Leica HyD's low dark noise, superior sensitivity and large dynamic range. BROADEN YOUR SCOPE SUPERIOR SIGNAL-TO-NOISE STANDARD QUANTITATIVE IMAGING The Leica HyD is designed to provide years of high performance. Due to its hybrid photo-detector design, the photocathode and downstream amplifying elements remain sensitive over time. Techniques borrowed from silicon chip manufacturing and a simplified...

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Hybrid Detector Technology – The Best of Two Worlds Photodetectors translate light into electric signal, which makes them a critical part of the recording process. Leica HyDs combine the best characteristics of the classic PMT and the highly sensitive avalanche photodiodes (APDs). This results in super-sensitivity and large dynamic range combined with rapid detection speed and low dark noise, making them an ideal detector for all samples. HYBRID DETECTOR TECHNOLOGY Photocathode Leica HyD photodetectors combine functional elements used in PMTs and APDs. At the photocathode, the arriving...

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LEICA HYD'S QUANTUM EFFICIENCY IS SUPERIOR TO STANDARD PMTS AND GaAsP PMTS Quantum efficiency is a measure for a detector’s capability Traditional PMTs using a GaAsP (Gallium-Arsenide-Phosphide) to translate photons into electrons. With a typical quantum photocathode are susceptible to damage by overexposure. efficiency of 45% at 500 nm, the hybrid detector is two to The Leica HyD’s design avoids this shortcoming, making three times more sensitive than a standard PMT. This new the detector both highly sensitive and versatile with a large level of sensitivity improves low-light applications...

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From Single Photons to Whole Organisms Photon counting enhances the dynamic range of an imaging system and opens the door to a new level of quantitative imaging. Enhanced dynamic range is achieved by preventing the dynamic overflow of single pixels or regions. Quantitative imaging benefits from photon counting as researchers can study photophysical processes and apparent concentrations of labeled molecules. The light emitted by a specific fluorophore is directly proportional to its concentration in the specimen. Photon counting provides for quantitative measurements such as ratio imaging,...

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Photon counts Photon counts Photon counts Photon counts MAXIMUM DYNAMIC RESOLUTION BY PHOTON COUNTING Due to the very low background of our hybrid detectors, photon counting allows as much information to be accumulated as needed for any statistical analysis. In photon counting each pixel behaves like a bucket, which can be filled with photons. The longer one counts, the more photons are collected. The higher bit-depth modes available, 12 bit and 16 bit, represent very large buckets: in 12 bit mode, one can fill 4096, in 16 bit 65356 photons into one pixel. Thus, an enormous dynamic range...

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Rat Cortical Neurons (Prim. Culture) Max. Projection Nuclei (Dapi, blue), Nestin (Cy2, green), DCX (Cy3, yellow), βIII-tubulin (Cy5, purple)

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Sensitivity by Design Photons emitted by the sample need to be preserved so they can contribute to your brilliant imagery. The Leica TCS SP8 provides high photon efficiency and gapless spectral detection using the innovation synergy of hybrid detectors, multiband spectral detector and acousto-optical beam splitter. SPECTRAL MULTIBAND SP DETECTOR YOUR CHOICE: DICHROIC BEAM SPLITTER OR AOBS The Leica HyD spectral detectors seamlessly integrate into Leica Microsystems offers two beam splitter technologies: the Leica SP detector module. This design offers simultaneous the acousto-optical beam...

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TCS SP8 HyD 24Pages

Catalog excerpts

All Leica Microsystems GmbH catalogs and technical brochures

TCS SP8 HyD
24Pages