THE VERTICAL TURN Leica TCS SP8 DLS Confocal and Digital LightSheet Microscope

Color coded three-dimensional image of eye specific GFP expression (3P3 promoter) in Drosophila melanogaster. Specimen courtesy of Nadja Dinges, Roignant Lab, IMB Mainz, Germany.

COMBINING LIGHT SHEET AND CONFOCAL – THE LEICA TCS SP8 DLS Your benefits at a glance: > Increase cell viability with single plane illumination > bserve fast live processes using an sCMOS-camera O > njoy easy sample handling and multi-position E experiments > Discover new fields of application by combining confocal methods with light sheet microscopy > Illuminate specimens from two sides to overcome darker regions > lways find the right imaging method for your A application by using the full capabilities of the Leica TCS SP8 > urn your confocal into a light sheet instrument: T Upgrade from Leica...

VERTICAL TURN, EXPANDED OPTIONS Our Leica TCS SP8 DLS combines a complete confocal microscope with gentle single plane illumination in one system. The innovative TwinFlect mirror has turned the Leica TCS SP8 confocal platform into an easy and versatile light sheet microscope. Benefit from THE VERTICAL TURN! More options for your research The innovative TwinFlect technology Living specimens – particularly developmental processes in embryos – are often Light sheet microscopy usually requires a very sensitive to light exposure. Illuminating your sample only in one single plane at dedicated optical...

High-end optics for demanding applications Superior objectives for a wide range of applications is one of Leica Microsystems’ hallmarks. The heart of our system turning the light sheet vertically is formed by the objectives and the TwinFlect mirror. With the choice of two different illumination objectives, the Leica HC PL Fluotar 2.5x/0.07 or the Leica HCX PL FLUOTAR 5x/0.15, you can shape the light sheet depending on the experiment's requirements. In order to reveal the finest details or have a larger field of view, you can pick the optimal detection objective, either the Leica HC FLUOTAR L...

Low phototoxicity and specimen imaging in 3D: Development of Drosophila melanogaster over 6 hours. Probe: Light-sensitive RFP. 3D rendering. 150 µm z stack, 30 sec/stack. Light sheet microscopy is a gentle way of imaging sensitive samples or fast biological processes by illuminating the specimen in only a single plane. Since there is no out-of-focus excitation, phototoxic effects can be reduced to the focal plane. It also means that you automatically have optical sectioning and you can image specimens in 3D by moving the sample through the light sheet. Light Sheet Microscopy Confocal Microscopy...

CONDUCT AND DOCUMENT LONG-TERM OBSERVATIONS Light sheet technology by Leica Microsystems allows you to observe the development of interesting organisms over a longer span of time using low illumination and high speed. Watching developments in real time and 3D Imaging requires light, but too much light can damage your cells. Light sheet microscopy is the most gentle imaging method to date, as it reduces the overall photodamage from phototoxicity and bleaching. This automatically increases the viability of your specimen. Particularly researchers in developmental biology benefit from light sheet...

FAST IMAGE ACQUISITION WITH EXCELLENT RESOLUTION Do you want to observe fast and periodic biological processes in three dimensions over an extended time period? Then the DLS module is the right choice. Discover the beauty of a beating zebrafish heart, for example. Track very fast periodic movements With the Leica TCS SP8 DLS you can choose between two cutting-edge sCMOS cameras for fast image acquisition with excellent resolution and high frame rates: The pco.edge sCMOS camera and the ORCA-flash 4.0 V2 by Hamamatsu are both fully integrated into the LAS X LightSheet Wizard. A new xytz scan mode...

High-speed imaging: Beating zebrafish heart, transgenic line flik1::EGFP. Top: transverse section at different time points. Bottom: Post-acquisition alignment after xytz recording. Recording speed: 120 frames/second. Courtesy of Emily Steed, Vermot Lab, IGBMC Strasbourg, France.

3:23:00.000 Long-term, multicolor observations: 17 hours of zebrafish lateral line development. Zebrafish 36hpf, CldnB:lynGFP / Cxcr4B:nuclearRFP. Sample courtesy of Darren Gilmour, EMBL Heidelberg, Germany. Simply switch between modes Our LightSheet module is more than a functional add-on to your confocal. The Leica TCS SP8 and Digital LightSheet synergize and give you the possibility to expand your options. You can easily manipulate your specimens using the confocal technology by simply switching between confocal and light sheet mode in LAS X software. Photoconversion or wounding experiments...

BENEFIT FROM MANY ADDITIONAL APPLICATIONS We have added light sheet technology to our confocal platform – you get two high performance microscopes in one. Always the right laser option Familiar sample preparation All visible lasers of your Leica TCS SP8 Due to the vertical experimental setup of the Leica TCS SP8 DLS, you can stick closely confocal are ready to be used for light to your routine sample preparation. The specimens are mounted in conventional glass sheet imaging. The highest flexibility in bottom petri dishes and are directly accessible. You even have the possibility of choosing the...

PARTNERS IN SCIENCE – WE FOCUS ON YOUR NEEDS Time is too precious to spend on complicated experimental set ups. That is why we focus on intuitive and convenient system design. Workflow-oriented software design The LAS X microscope software guides users step by step through data recording and evaluation. The workflow-oriented design helps you to use the instrument more efficiently. A convenient calibration routine establishes the light sheet precisely. Dual-sided illumination of the sample comes by design: Each of the two opposing mirrors of the TwinFlect can be targeted by the scanner to overcome...