REDEFINING THE DETECTION LIMIT OF CONFOCAL IMAGING Lightning Image Information Extraction Chromosomes: The LIGHTNING detection system for image information extraction ensures the clear and unobstructed view of the relevant information One Click Ahead: The Maximum of Information from Each Specimen Maximize the information you extract from your specimen and get in-depth answers to scientific questions – with the LIGHTNING detection package for image information extraction. What is more, the LIGHTNING detection concept extends the TCS SP8 confocal microscope by simultaneous multicolor super-resolution imaging at the full original speed. LIGHTNING works with a decision mask that takes individual volume segments into account which is remarkable superior to a global processing. LIGHTNING fully automatically detects the finest structures and details, which are otherwise simply not visible. The key for LIGHTNING is an adaptive process for extraction of hidden information in the image. Unlike traditional technologies, that use a global set of parameters for the full image, LIGHTNING calculates an appropriate set of parameters for each voxel to uncover every detail with the highest fidelity. In this process, the LIGHTNING decision mask ensures to adapt the best fitting reconstruction strategy for each specific volume element (see middle right image).

Reproducible Results with Optimal Settings Confocal imaging with the adaptive intelligence technology of LIGHTNING optimizes the image for every probed location in the specimen. The final set of parameters is stored with each image so the imaging conditions are fully documented and, as a result, reproducible. >> Set up all resolution-relevant parameters using one slider. >> o post-processing needed due to full integration of N LIGHTNING in the online image acquisition >> anage large data sets created by time-lapse recording, M Mark&Find, Tile Scan or Navigator. >> Visualize LIGHTNING 3D data...