FIND THE MISSING LINKS DMi8 S Advanced Live Cell Imaging Solution

DMi8 S – ADVANCED LIVE CELL IMAGING SOLUTION

See what you’ve been missing The DMi8 S is a complete solution for advanced widefield research. The research process begins with the discovery and analysis of single molecules. From there, piecing together interactions between complexes and signaling pathways leads to the analysis of complete systems – culminating in breakthroughs in understanding and treating human health. The key to the next scientific discovery lies in finding the missing links connecting your data. Whether you need to precisely follow the development of a single cell in a dish, screen through multiple assays, obtain single...

SEE MORE Overview scan of mouse brain section with proliferating cells (BrdU – red), cell nuclei (Dapi – blue), and mature neurons (NeuN – green) stained, with two regions of interest selected for higher resolution tile scans. Courtesy of Dr. Wei Mo, School of Life Sciences, Xiamen University, China. LAS X NAVIGATOR SOFTWARE INCREASE YOUR VIEWING AREA UP TO 10,000X See more of the links you’ve been missing Switch from searching image by image to seeing the full overview of your samples. Like a GPS for your cells, LAS X Navigator ensures that you always have a clear roadmap to brilliant data....

Herovici staining of human skin with scar tissue. Courtesy of Dr. Swathi Balaji, Baylor College of Medicine, Houston, Texas, USA. Find your answers No matter what experiments you have in mind, LAS X Navigator is the key to all applications on your DMi8 S imaging system. Generate overviews from your live image Create spiral scans to search in the vicinity of your current location Display images in sample carrier templates for quick orientation Use any magnification, camera, detector, and contrasting method in the same workspace Define an unlimited number of regions and positions for high resolution...

SEE FASTER Three color immunostained coronal section of mouse nasal cavity. DMi8 S POWERED BY LAS X SYNAPSE CAPTURE MORE DETAILS WITH UP TO 5X FASTER EXPERIMENTS Find the missing links in your data faster Utilize the LAS X Synapse advanced sequencer to follow events like vesicle trafficking and movement of kinesin motors along axonemes in simultaneous multi-color TIRF fluorescence at multiple stage positions over time. No matter which of the instrument components are part of the experiment, the system will run at the highest speed possible. The DMi8 S imaging solution with LAS X Synapse software...

Hela cells stably expressing Actin Chromobody-TagGFP2 and SIR-Lysosome. Overlay of three separately colored timepoints collected using Hi-Lo illumination showing lysosomal movements. Chromobody-TagGFP2 imaged using TIRF. Courtesy of ChromoTek GmbH, Munich, Germany, and Spirochrome SA. Use the DMi8 S with the advanced sequencer to set up dynamic experiments examining cellular responses. Precisely synchronize your functional imaging to external stimuli. External sensor data (black) used to control imaging by sending stimuli triggers (red) and receiving imaging triggers (blue). Full synchronization...

SEE THE HIDDEN Hela cells stably expressing Actin Chromobody-TagGFP2 and stained with SIR-Tubulin. Courtesy of ChromoTek GmbH, Munich, Germany, and Spirochrome SA. DMi8 S INFINITY TIRF SIMULTANEOUS MULTI-COLOR TIRF & SUPER-RESOLUTION MODULE Reveal the missing links in your cells Observe samples with outstanding clarity, control, and confidence with the DMi8 S Infinity TIRF module. For dynamic processes at the cell surface, TIRF (Total Internal Reflection Fluorescence) microscopy is the method of choice to visualize single molecules with super-resolution by maximizing the fluorescent signal-to-noise...

In vitro tracking experiment of Kinesin-2 from c.elegans at saturating ATP concentration. 11 subunit labeled with Snap-Alexa 488, 20 subunit labeled with Halo-660. Courtesy of Willi Stepp, Technical University of Munich, Germany. Usability and reproducibility Take the mystery out of setting up a TIRF experiment with the fully automated Infinity TIRF. Sample-specific alignment and calibration via integrated sensors ensure that your results are consistent and reproducible. Full integration with LAS X software helps you minimize setup and training time so that you can get to what really matters:...

SEE THE HIDDEN Hela cells stably expressing Actin Chromobody-TagGFP2. Courtesy of ChromoTek GmbH, Munich, Germany. DMi8 S INFINITY SCANNER ACTIVATE, ABLATE, AND BLEACH IN ONE EXPERIMENT Unmask the links hiding within your cells Whether you need to bleach, cut, activate, stimulate or even combine multiple techniques, the Infinity Scanner can be configured to address your needs. The Infinity Scanner modules offer the ultimate multispectral photo manipulation tool designed to deliver unparalleled application flexibility. High speed vector scanning capabilities allow you to not only have precise...

Dendra2-H2B photoswitching. Courtesy of Dr. Rebecca Smith, Physiological Chemistry, Biomedical Center, Ludwig-Maximilians-University, Martinsried, Germany. LAS X Software integration The Infinity Scanner is fully integrated into the LAS X software, so planning and executing even complex experiments is simple. Using the LAS X interface, set up multiple laser powers and channels for techniques like FRAP, FLIP, acceptor bleaching, activation, switching, optogenetics, DNA damage, cutting, ablation, or uncaging within a single experiment. Tunable Photomanipulation With dual optical pathways, the Infinity...

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