Toggel
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Toggel Toggel is a next-generation fluorescence lifetime imaging microscopy (FLIM) camera that simplifies FLIM for researchers and imaging centers by combining excellent light sensitivity with easy image acquisition and data analysis. With Toggel, Lambert Instruments aims to minimize measurement duration, automate image acquisition and simplify data analysis. These factors are of great importance to many of our customers in cell biology, cancer research and high-throughput screening. Toggel features a unique image sensor that combines excellent light sensitivity with advanced fluorescence lifetime imaging capabilities. This image sensor was designed and optimized specifically for fluorescence lifetime imaging applications and enables lifetime imaging at unprecedented frame rates with the single-image fluorescence lifetime imaging microscopy (siFLIM) method [1]. Imaging modes of the camera include regular frequency-domain FLIM acquisition and time-lapse recordings. Data analysis is done automatically by the Lambert Instruments software, which instantly calculates the fluorescence lifetime and presents it to the user as a colorized overlay on the original image. KEY FEATURES Easy coupling Flexible and efficient lens coupling to all major-brand microscopes with C-mount or F-mount camera ports. High-resolution image sensor For sharp fluorescence lifetime images. Wide range of available modulation frequencies Up to 80 MHz. Compact design For an easy fit to your imaging or microscopy setup. Specifications are subject to change without prior notice. APPLICATIONS Fluorescence Lifetime Imaging Fluorescence Resonance Energy Transfer Spinning-Disk Confocal Total Internal Reflection Fluorescence Hyperspectral imaging Next-Generation FLIM Camera

Applications Top row: Light intensity images (colorized). Bottom row: Corresponding fluorescence lifetime images (colorized). The average fluorescence lifetime of the cells increases over time, as shown in the graph on the right. Image courtesy of the Netherlands Cancer Institute. B. subtilis cells showing different lifetimes. Top row: Original lifetime images of FRETing, non-FRET-ing and mixed cells. Bottom row: Same cells as top row, but categorized and colorized based on average cell lifetime. Scale bar is 5 pm. Image courtesy of University of Groningen [2]. References [1] siFLIM:...