LIFA Widefield
6Pages

Fluorescence Lifetime Attachment The LIFA is a dedicated system for Fluorescence Lifetime Imaging Microscopy (FLIM). It allows the generation of lifetime images on any widefield fluorescence microscope using frequency domain technology. The LIFA model consists of a TRiCAM M modulated intensified CCD camera, a modulated lightsource, a modulation control unit, a personal computer and the LI-FLIM software package. The intensified camera contains an image intensifier that is fiber-optically coupled to the CCD camera for maximum gain and speed. Easy coupling Flexible and efficient coupling to all major-brand fluorescence microscopes. Fluorescence Lifetime Imaging Microscopy (FLIM) Fast acquisition Record lifetime images in a matter of seconds. Protein Interactions High resolution image intensifiers Gen II and Gen III image intensifiers offering the world’s highest resolution and sensitivity in the UV, visible or near infrared. Accurate lifetime determination Fluorescence lifetimes from 0–300 ns with an accuracy of 30 ps r.m.s. Specifications are subject to change without prior notice. FRET Efficiency Mapping Biosensors Oxygen Concentration Imaging NADH/FAD Fluorescence Dynamics Membrane Dynamics Molecular Interactions

SPECIFICATIONS Specifications Typical value System Performance Lifetime range Lifetime resolution less than 100 ps (single pixel) 2 lifetime images per second (full frame) Spatial resolution single photon Intrascene dynamic range Camera input format Effective pixel size 10.3 µm square (12,9 for lens coupled CCD) CONTROL UNIT The control unit is used to generate and control the modulation signals for the lightsource and the intensified camera. It also contains the high voltage power supply generating the DC voltages for the image intensifier. All variables are controlled in the LI-FLIM...

MULTI-LED LIGHT SOURCE The Multi-LED is a modulated light source designed for fluorescence excitation. In the Multi-LED, up to four LED units are mounted having one common optical output to the microscope. Available LEDs cover the wavelength range from 360 to 660nm. The unit is cable-connected to the LIFA control unit that delivers the DC and AC power to the selected LED. LED selection, DC current, modulation frequency, amplitude and phase are controlled via the user interface of the LI-FLIM software. Optionally the Multi-LED can be temperature controlled for improved long-term stability....

TheTRiCAM M is the modulated version of the TRiCAM modulated intensified camera manufactured by Lambert Instruments. The TRiCAM is a compact camera based on a 18 mm MCP image intensifier that can be gain-modulated at high frequencies to allow fre- quency-domain imaging. The intensifier can also be operated DC to allow focusing and selecting the object of interest. Optionally, the TRiCAM can be provided with gating to allow time-gated imaging. AC and DC voltages are provided from the control unit via a cable that is permanently connected to the camera for maximum performance. The output of...

2/3” interline, progressive scan Pixel size Dynamic range Synchronized with Control Unit Integration time control Fastest mode (4x4 binning = 348x256) Digital output External trigger input Trigger output Pixel clock Programmable camera gain High light level protection Environmental temperature Lens mount Image intensifier light sensitivity 225

PERSONAL COMPUTER (MINIMAL SPECIFICATION) Operating system Hard disk System includes DVD R, IEEE1394, LAN/Ethernet, USB2.0, keyboard, optical mouse Software LI-FLIM Acquisition and lifetime modules, full digital control of hardware, viewer and image analysis tools. External control via ActiveX. Software Development Kit for CCD camera and Control Unit Offers full programmatic (C/C++) control of camera and of Control Unit functionality. Lambert Instruments BV Oosteinde 16, 9301 ZP Roden The Netherlands www.lambertinstruments.com info@lambertinstruments.com Tel: +31-(0)50-501 8461 Fax:...