LIFA Specification
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Catalog excerpts

LIFA Specification - 1

The LIFA is a dedicated system for Fluorescence Lifetime Imaging Microscopy (FLIM). It allows the generation of lifetime images on any widefield fluorescence microscope using frequency domain technology. The LIFA model consists of a TRiCAM M modulated intensified CCD camera, a modulated lightsource, a modulation control unit, a personal computer and the LI-FLIM software package. The intensified camera contains an image intensifier that is fiber-optically coupled to the CCD camera for maximum gain and speed. Fluorescence Lifetime Attachment SPECIFICATIONS Specifications System Performance Lifetime range Lifetime resolution less than 100 ps (single pixel) 2 lifetime images per second (full frame) Spatial resolution single photon Intrascene dynamic range Camera input format Effective pixel size 10.3 µm square (12,9 for lens coupled CCD) CONTROL UNIT The control unit is used to generate and control the modulation signals for the lightsource and the intensified camera. The unit also contains the high voltage power supply generating the DC voltages for the image intensifier. All variables are controlled in the LI-FLIM software via a USB 2.0 interface. Signal shape Frequency range Frequency adjustment resolution Frequency deviation Harmonics suppression Output 1 Type a: MultiLED AC signal level DC current adjust capable of driving lightsource type a, optionally b and c adjustable in 250 steps 0 – 1000 mA, 12 bit resolution

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LIFA Specification - 2

Optional lightsource outputs: Type b: Modulated laser diode AC signal level DC level adjust Type c: AOM for CW laser AC signal level Frequency 0.00 – 1.00 V peak-peak half the frequency of output 2 Output 2 AC signal level AC signal after amplification to image intensifier adjustable from –50 to 0 dBm 15 Vpp nom. External trigger input LVTTL (phase shift on falling edge or rising edge) Phase adjustment range Phase deviation MULTI-LED LIGHT SOURCE TYPE A The Multi-LED is a modulated light source designed for fluorescence excitation. In the Multi-LED, up to four LED units are mounted having...

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LIFA Specification - 3

TRICAM M INTENSIFIED CCD CAMERA The TRiCAM M is the modulated version of the TRiCAM modulated intensified camera manufactured by Lambert Instruments. The TRiCAM is a compact camera based on a 18 mm MCP image intensifier that can be gainmodulated at high frequencies to allow frequency-domain imaging. The intensifier can also be operated DC to allow focusing and selecting the object of interest. Optionally, the TRiCAM can be provided with gating to allow timegated imaging. AC and DC voltages are provided from the control unit via a cable that is permanently connected to the camera for maximum...

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LIFA Specification - 4

The number of spots exceeding a contrast with their surrounding area of 30% is less than or equal to the number indicated in the table below. The size of non-circular spots is determined on the basis of equal area to circular spots. When the distance between two spots is less than the maximum dimension of either spot, the two spots are considered to be one spot. Gen II Max. number of spots Size of spots within effective area Max. number of spots within effective area Dark spots White spots 2/3” interline, progressive scan Pixel size Dynamic range Max. frame rate at full resolution...

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LIFA Specification - 5

Software LI-FLIM Acquisition and lifetime modules, full digital control of hardware, viewer and image analysis tools. External control via ActiveX Software Development Kit for CCD camera and Control Unit Offers full programmatic (C/C++) control of camera and of Control Unit functionality MULTI-LASER TYPE B (OPTIONAL) The modulated laser diodes of the Multi-LASER offer the high stability and high modulation depth required for highaccuracy lifetime imaging, and can also be used for regular fluorescence intensity imaging. The Multi-LASER uses a kineFLEX fiber delivery system to easily couple...

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