Why Fluorescence Lifetime Imaging Microscopy? Upon excitation, fluorescent molecules emit light that decays exponentially with time according to the decay rates of their excited state. The corresponding fluorescence lifetime is a telltale signature of the molecules and their immediate environment. FLIM - Fluorescence Lifetime Imaging Microscopy - is the technique to map the spatial distribution of lifetimes in living cells and in inorganic material. A key advantage of the fluorescence lifetime above the intensity is that fluorescence lifetime is independent of concentration, bleaching and intensity...

The LIFA: Frequency Domain FLIM The LIFA is a camera-based FLIM system for fast fluorescence and/ or phosphorescence lifetime imaging in the frequency domain, and is compatible with Leica®, Nikon®, Olympus® and Zeiss® fluorescence microscopes. Its well-established homodyne detection technology together with the parallel detection offered by the TRiCAM modulated camera allows near instantaneous acquisition of full field lifetime images at high accuracy. As it is a camera-based system the LIFA is especially well-suited for live cell imaging. The standard, widefield system includes a Multi-LED modulated...

LIFA COMPONENTS TRiCAM modulated ICCD camera Multi-LASER and/or Multi-LED light source(s) Control unit (includes modulation signal generator and high voltage power supply) LI-FLIM software package USB 2.0 interface Personal computer and 24” monitor Installation, training and support LIFA SPECIFICATIONS Lifetime range 0-300 ns (LIFA) 0-1 ms (LIFA-X) 300 ns - 1ms (LIFA-P) Lifetime resolution Lifetime drift Modulation frequency 1-120 MHz (LIFA) / 0,1 KHz - 120 MHz (LIFA-X) / 0,1 KHz - 100 KHz (LIFA-P) Two full-frame lifetime images per second Spatial resolution during modulation 26 lp / mm or approximately...