LIFA-FLIM Fluorescence Lifetime Imaging Microscopy
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Catalog excerpts

Why Fluorescence Lifetime Imaging Microscopy? Upon excitation, fluorescent molecules emit light that decays exponentially with time according to the decay rates of their excited state. The corresponding fluorescence lifetime is a telltale signature of the molecules and their immediate environment. FLIM - Fluorescence Lifetime Imaging Microscopy - is the technique to map the spatial distribution of lifetimes in living cells and in inorganic material. A key advantage of the fluorescence lifetime above the intensity is that fluorescence lifetime is independent of concentration, bleaching and...

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The LIFA: Frequency Domain FLIM The LIFA is a camera-based FLIM system for fast fluorescence and/ or phosphorescence lifetime imaging in the frequency domain, and is compatible with Leica®, Nikon®, Olympus® and Zeiss® fluorescence microscopes. Its well-established homodyne detection technology together with the parallel detection offered by the TRiCAM modulated camera allows near instantaneous acquisition of full field lifetime images at high accuracy. As it is a camera-based system the LIFA is especially well-suited for live cell imaging. The standard, widefield system includes a Multi-LED...

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LIFA COMPONENTS TRiCAM modulated ICCD camera Multi-LASER and/or Multi-LED light source(s) Control unit (includes modulation signal generator and high voltage power supply) LI-FLIM software package USB 2.0 interface Personal computer and 24” monitor Installation, training and support LIFA SPECIFICATIONS Lifetime range 0-300 ns (LIFA) 0-1 ms (LIFA-X) 300 ns - 1ms (LIFA-P) Lifetime resolution Lifetime drift Modulation frequency 1-120 MHz (LIFA) / 0,1 KHz - 120 MHz (LIFA-X) / 0,1 KHz - 100 KHz (LIFA-P) Two full-frame lifetime images per second Spatial resolution during modulation 26 lp / mm or...

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