HiCAM_Fluo
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HiCAM Fluo HiCAM Fluo The HiCAM Fluo is a versatile streaming camera system designed for fluorescence microscopy. It features a 1.3 megapixel image sensor that captures detailed images at high frame rates. Thanks to the integrated fiber-coupled image intensifier, it operates under low-light conditions, making it the ideal camera for high-speed fluorescence microscopy. Cooling of the intensifier significantly reduces the amount of noise. With its gated image intensifier, the camera’s effective exposure time can be reduced. The minimum gate width of 40 ns increases the range of light levels at which the camera can be used. It also eliminates motion blur and enables time-resolved filtering. The fanless design of the camera minimizes vibrations to ensure sharp images. Very low noise levels are achieved by Peltier cooling the image intensifier. Noise levels are reduced by a factor of up to 100 times as compared to uncooled intensified cameras. Images are streamed to a computer using a CoaXPress interface. This high-speed interface enables recording times limited only by hard disk space. Linking the camera to a framegrabber with on-board FPGA enables realtime analysis of the streamed data. Specifications are subject to change without prior notice. HiCAM Fluo 17001A01 17/03/2017 Cooled High-Speed Fluorescence Camera Easy coupling: Flexible and efficient lens coupling to all major-brand microscopes with C-mount or F-mount camera ports. High-resolution image intensifiers: Gen III image intensifiers for the highest resolution and sensitivity in the UV, visible or NIR. Short gate pulse widths: Down to 40 ns (FWHM) with minimal jitter. High gate repetition rates: Up to 100 kHz Compact design: For an easy fit to your imaging or microscopy setup. High-speed Fluorescence Imaging Bio- and Chemiluminescence Imaging Time-resolved imaging and spectroscopy Laser Induced Fluorescence (LIF) Particle Image Velocimetry (PIV) Plasma physics Microfluidics Combustion

Gain Control Gate control Anode current limiter Internal trigger generator Shutter control Gating pulse width range Minimal pulse width (jitter) Pulse width increments Pulse repetition rate Optional < 3 ns - 10 s < 3 ns (< 80 ps RMS) 10 ps 300 kHz, 2.5 MHz burst mode

This cloud of electrons is accelerated towards the anode screen, where the electrons are converted back into photons by the phosphor layer. These photons are transferred to the camera by a fiberoptical taper. Intensifier sensitivity Phosphor response time IMAGE INTENSIFIER PROPERTIES Image intensifier Proximity-focused Gen III GaAsP (filmless) Spatial resolution bare intensifier Input window Borosilicate glass 18 mm Gen III intensifier Effective area on input Input window thickness Photons are converted into electrons at the photocathode. These are accelerated towards the micro-channel...

Frames from a 2000 fps recording of the heart of a zebrafish. On average, the heart of a zebrafish beats 2 to 3 times a second. By labelling the blood cells with a DS_red fluorescent dye, detailed images of the various stages of one heartbeat can be recorded. Images of a lily of the valley sample without (left) and with (right) image intensifier cooling. www.lambertinstruments.com info@lambertinstruments.com