HiCAM Fluo
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HiCAM Fluo HiCAM Fluo 15001A01 19/02/2015 Cooled High-Speed Fluorescence Camera The HiCAM Fluo is a versatile streaming camera system designed for fluorescence microscopy. It features a 3 megapixel image sensor that captures detailed images at high frame rates of up to 1000 fps at 1200 x 1200 pixels. Thanks to the integrated fiber-coupled image intensifier, it operates under low-light conditions, making it the ideal camera for highspeed fluorescence microscopy. Cooling of the intensifier significantly reduces the amount of noise. With its gated image intensifier, the camera’s effective exposure time can be reduced. The minimum gate width of 3 ns (FWHM) increases the range of light levels at which the camera can be used. It also eliminates motion blur and enables time-resolved filtering. The fanless design of the camera minimizes vibrations to ensure sharp images. Very low noise levels are achieved by Peltier cooling the image intensifier. Noise levels are reduced by a factor of up to 100 times as compared to uncooled intensified cameras. Images are streamed to a computer using a CoaXPress interface. This high-speed interface enables recording times limited only by hard disk space. Linking the camera to a framegrabber with on-board FPGA enables realtime analysis of the streamed data. Easy coupling Flexible and efficient lens coupling to all major-brand microscopes with C-mount or F-mount camera ports. High-speed Fluorescence Imaging High resolution image intensifiers Gen III image intensifiers offer the world’s highest resolution and sensitivity in the UV, visible or near infrared. Short gate pulse widths Down to 40 ns (FWHM) with minimal jitter. High gate repetition rates Up to 100 kHz Compact design For an easy fit to your imaging or microscopy setup. Particle Image Velocimetry (PIV) Single photon imaging Time-resolved imaging and spectroscopy Laser Induced Fluorescence (LIF) Combustion Plasma physics Astronomy Specifications are subject to change without prior notice.

CAMERA SPECIFICATION Maximum resolution (pixels) Resolution (pixels) Minimum exposure time Pixel size Computer interface HiCAM Fluo GATING PROPERTIES Gain Control Gate control Anode current limiter Internal trigger generator Shutter control Gating pulse width range Minimal pulse width (jitter) Pulse width increments Pulse repetition rate

This cloud of electrons is accelerated towards the anode screen, where the electrons are converted back into photons by the phosphor layer. These photons are transferred to the camera by a fiberoptical taper. Intensifier sensitivity Phosphor response time IMAGE INTENSIFIER PROPERTIES Image intensifier Proximity-focused Gen III GaAsP (filmless) Spatial resolution bare intensifier Input window Borosilicate glass 18 mm Gen III intensifier Effective area on input Input window thickness Photons are converted into electrons at the photocathode. These are accelerated towards the micro-channel...

Frames from a 2000 fps recording of the heart of a zebrafish. On average, the heart of a zebrafish beats 2 to 3 times a second. By labelling the blood cells with a DS_red fluorescent dye, detailed images of the various stages of one heartbeat can be recorded. Images of a lily of the valley sample without (left) and with (right) image intensifier cooling. www.lambertinstruments.com info@lambertinstruments.com