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Light Scattering Detector ELSD 3300

Light Scattering Detector ELSD 3300

Product catalog summary
Overview: The Light Scattering Detector ELSD 3300 is designed for sensitive and universal HPLC detection, independent of eluent composition. It is suitable for routine analysis and research, particularly for non-UV active compounds such as sugars, fats, surfactants, polymers, pharmaceuticals, amino acids, peptides, and natural compounds.
Detection Principle: The ELSD 3300 operates on a three-step principle: 1) Nebulization of the column effluent in nitrogen using a jet, 2) Evaporation of the mobile phase in a heated zone, leaving a mist of sample particles, 3) Detection of scattered light as sample particles interfere with a laser light beam. This allows for drift-free detection of gradient runs without complex setups.
Technical Specifications:
  • Light Source: Laser diode with collimating optics, 650 nm, 30 mW output, class IIIb.
  • Detector: Silicon photo diode.
  • Sensitivity: Limit of Detection (LOD) for butyl paraben is 25 ng.
  • Nebulizer: Covers a wide flow range without hardware modifications, using nitrogen gas at a flow rate of 4 l/min and inlet pressure of 60 - 80 psig (4.0 - 5.5 bar).
  • Operating Range: Typical 1.0 - 2.0 l, HPLC flow rate 0 - 3.0 ml/min.
  • Heated Zone: Temperature range from ambient to 120°C in 1°C increments.
  • Analog Output: Selectable between 0 - 1 V or 0 - 10 mV.
  • Control and Data Ports: RS-232, USB, and Ethernet available.
  • Communication: Inputs for auto zero, gas shutoff, start, and standby; outputs for pump shutdown and general events.
  • Display/Operation: Graphical color LCD with live signal curve view, 7-language user interface, help function, and integrated method setup wizard.
  • Power Supply: 120 / 240 V, 50 / 60 Hz.
  • Dimensions/Weight: 262 x 295 x 495 mm (W x H x D) / 16.0 kg.
Ordering Information: Model A0925-1, suitable for detecting UV inactive substances such as carbohydrates, surfactants, polymers, natural compounds, and pharmaceuticals in HPLC systems.
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Catalog excerpts

Light Scattering Detector ELSD 3300-1

Light Scattering Detector ELSD 3300 Sensitive, Universal HPLC Detection, Independent of Eluent Composition. The Light Scattering Detector ELSD 3300 has a wide range of applications in routine analysis and research. Common fields of application for the ELSD 3300 include the analysis of non-UV active compounds such as: sugars, fats, surfactants, polymers, pharmaceuticals, Amino acids / Peptides, Natural compounds. Three-Step-Principle: 1) Nebulization of the column effluent in nitrogen by means of a jet. 2) Evaporation of the mobile phase in a heated zone leaving a fine mist of sample particles. 3) Detection of the scattered light, when the sample particles pass and interfere the laser light beam. Gradient runs of variable slope and composition can be detected drift free without complicated setup procedures, because vaporization of the mobile phase occurs before detection takes place. Light Source Laser diode with collimating optics, 650 nm, 30 mW output, class IIIb Detector Silicon photo diode Sensitivity (Limit of Detection, LOD) butyl paraben, 25 ng Nebulizer Gas nitrogen preferred Gas Flow Rate calibrated to 4 l/min Gas Inlet Pressure 60 - 80 psig (4.0 - 5.5 bar) Operating Range typical 1.0 - 2.0 l HPLC Flow Rate 0 - 3.0 ml/min Maintenance easily accessible from the front if cleaning required Heated Zone Important Note: Covers a wide flow range without the need for hardware modifications. According to current information, the most sensitive ELSD on the market. Integrated method setup wizard. Temperature Range ambient to 120°C in 1°C increments Analog Output selectable between 0 - 1 V or 0 - 10 mV Control and Data Ports RS-232 (furthermore USB and Ethernet available) Communication Inputs TTL/contact closure for auto zero, gas shutoff, start, and standby Outputs contact closure for pump shutdown and general events Display / Operation graphical color LCD incl. live view of signal curve, 7-language user interface, help function, integrated method setup wizard / alphanumerical keypad Power Supply 120 / 240 V, 50 / 60 Hz Dimensions / Weight 262 x 295 x 495 mm (W x H x D) / 16.0 kg Technical data is subject to change without notice. Ordering Information A0925-1 Light Scattering Detector ELSD 3300 sensitive, gradient capable ELSD for detection of UV inactive substances such as carbohydrates, surfactants, polymers, natural compounds, or many pharmaceuticals in HPLC systems

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