Overview Analytical HPLC and UHPLC columns Eurospher II for HPLC, HPLC Plus and UHPLC Eurokat for LC separation of carbohydrates and organic acids • Handy column hardware -> easy exchange of filters • Integrated precolumn system -> no loss in separation power • Wide area of application services -> column- and method guidance KNAUER Wissenschaftliche Geräte GmbH · Hegauer Weg 38 · 14163 Berlin · www.kna

Characterisation of phases based on Tanaka tests Optimized selectivity – for better separations hydrophobic retention hydrophobic selectivity steric selectivity hydrogen bonding capacity ion exchange capacity at pH 7.6 ion exchange capacity at pH 2.7 Eurospher II 100 C18 - USP L1 for acidic, basic and neutral analytes in reversed phase mode (sulphonamides; anabolic steroids; anti-psychotics; beta blocker; Sudan dyes; phenols, preservatives etc.) Eurospher II 100 C18 A - USP L1polar endcapped C18 phase for alternative selectivity; 100% aqueous applications with very polar compounds (basic pharmaceutical...

Eurospher II 100 C8 - USP L7 similar selectivity to C18 phase but less retention due to the lower hydrophobicity; useful for determination of water soluble vitamins, steroids, catecholamines, sedatives etc. Eurospher II 100 Phenyl - USP L11 alternative selectivity for aromatic and mode rately polar analytes or mixtures with varying polarity and aromaticity Eurospher II 100 HILIC especially suited for the separation of hydrophilic, polar and ionic analytes which are only poorly retained on reversed phase columns; behavior is the other way round on Eurospher II HILIC compared to RP which makes...

Eurospher II Family Based on an ultra pure spherical silica gel, Eurospher II is a high performance column material for analytical, semipreparative and process-scale applications. Eurospher II features very narrow particle and pore size distributions, as well as outstanding mechanical stability. Eurospher II silica gel is perfectly suited to take on routine analyses as well as the most ambitious chromatography tasks. Especially for ultrafast UHPLC, Eurospher II is also available in 2 pm particle size. Characteristics: • Eurospher II 100 HPLC phases are based on ultra pure 3rd generation silica...

Eurospher II 100 C18 - USP LI -Ultra pure, spherical high performance HPLC phase based on silica gel Unpolar, monomeric C18 (Octadecyl) modification, endcapped, 16 % carbon content (~ 50 % endcapping). Properties: Separation mechanism: Hydrophobic interaction Key features: High-class HPLC phase perfectly suited to take on routine analyses as well as the most ambitious chromatography tasks, classical C18 phase with ca. 50 % endcapping and resulting 16 % carbon content, outstanding mechanical and chemical stability Technical data: C18 phase based on ultra-pure silica gel (purity > 99 %), metal...

Eurospher II 100 C18 A - USP L1-Ultra pure, spherical high performance HPLC phase based on silica gel Polar, monomeric C18 (Octadecyl) modification, polar endcapping, 10 % carbon content (~ 50 % endcapping) Properties: Separation mechanism: Hydrophobic and polar interaction Key features: C18 A phase with alternative, more polar selectivity, suited for the use with pure aqueous mobile phases, 50 % endcapping and resulting 10 % carbon content, outstanding mechanical and chemical stability, suited for analytical as well as semi preparative and preparative applications Technical data: C18 A phase...

Eurospher II 100 C18 H - USP LI -Ultra pure, spherical high performance HPLC phase based on silica gel Unpolar, monomeric C18 (Octadecyl) modification, high efficiency endcapping, 17 % carbon content (~ 99 % endcapping) Properties Separation mechanism: Hydrophobic interaction Key features: Unpolar C18 phase with high efficiency endcapping and 17 % carbon content, therewith higher pH stability in the range of 1-12, outstanding mechanical and chemical stability, suited for analytical as well as semi preparative and preparative applications Technical data: C18 phase based on ultra-pure silica gel...

Eurospher II 100 C18 P - USP LI -Ultra pure, spherical high performance HPLC phase based on silica gel Unpolar, trifunctional C18 (Octadecyl) modification, high efficiency endcapping 20 % carbon content (~ 99 % endcapping) Properties Separation mechanism: Hydrophobic and steric interaction Key features: KNAUER's most unpolar C18 phase, polymeric, high efficiency endcapping of 99 % and 20 % carbon content, higher pH stability in the range of 1-12, outstanding mechanical and chemical stability, suited for analytical as well as semi preparative and preparative applications Technical data: C18 phase...

Eurospher II 100 C8 - USP L7 -Ultra pure, spherical high performance HPLC phase based on silica gel Monomeric C8 (Octyl) modification, standard endcapping 10 % carbon content (~ 50 % endcapping) Properties Separation mechanism: Hydrophobic interaction (lower compared to C18 phases) Key features: Classical C8 phase, standard 50 % endcapping and 10 % carbon load, outstanding mechanical and chemical stability, suited for analytical as well as semi preparative and preparative applications Technical data: C8 phase based on ultra-pure silica gel (purity > 99 %), metal content < 10 ppm, pH-stability...

Eurospher II 100 Phenyl - USP L11 -Ultra pure, spherical high performance HPLC phase based on silica gel Phenyl modification (Phenylpropyl), 12 % carbon content Properties Separation mechanism: Pi-Pi Interactions Key features: Classical phenyl phase with 12 % carbon load, outstanding mechanical and chemical stability, suited for analytical as well as semi preparative and preparative applications Technical Data: Phenyl phase based on ultra-pure silica gel (purity > 99 %), metal content < 10 ppm, pH-stability 2 - 8, pore size 100 A, particle size 2 pm, 3 pm, 5 pm, 10 pm (15 pm, 20 - 45 pm on request). Recommended...

Eurospher II 100 HILIC Ultra pure, spherical high performance HPLC phase based on silica gel Zwitterionic modification: Ammonium – sulfonic acid, 7 % carbon content Properties Separation mechanism: Hydrophilic and weak electrostatic interactions Key features: Modern HILIC phase with zwitterionic modification on the basis of ammonium- sulfonic acid, outstanding mechanical and chemical stability Technical Data: HILIC phase based on ultra-pure silica gel (purity > 99 %), metal content < 10 ppm, pH-stability 2 - 8, pore size 100 A, particle size 3 μm and 5 μm Recommended application areas: Especially...