Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometer Serving Advanced Technology

Unrivaled SpiralTOF Technology The JMS-S3000 is a MALDI-TOFMS* incorporating an innovative SpiralTOF ion optical system. The JMS-S3000 defines a new standard in MALDI-TOFMS performance and provides state-of-the-art analytical solutions for a wide range of research areas such as functional synthetic polymers, materials science, and biomolecules. * Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometer 2 SpiralTOF

Flexible configurations for your application The JMS-S3000 is available in four configurations depending on the combination of two options; TOF-TOF and Linear TOF. JMS-S3000 The JMS-S3000 is a MALDI-TOFMS with unsurpassed high mass-resolving power and high mass accuracy through its SpiralTOF technology. A solid-state laser is employed for achieving high throughput and low running cost. JMS-S3000 TOF-TOF option With TOF-TOF option, precursor ions selected by SpiralTOF can be fragmented through highenergy collision-induced dissociation (high-energy CID). Product ions produced by high-energy CID...

SpiralTOF: time-of-flight ion optics reinvented JEOL continues the quest to enhance the performance of TOFMS. To improve the mass resolving power and mass accuracy of a time-of-flight mass spectrometer, it is required to extend the flight distance while keeping a group of ions having the same m/z (an ion packet) from diverging in space. Simply extending the flight distance of the conventional linear or reflectron TOFMS makes the physical size of the instrument larger and reduces the sensitivity due to angular diversion of the ion packets while the mass resolving power may not improve due to the...

The SpiralTOF ion optical system adopted for the JMS-S3000 sur- One figure-eight trajectory passes the conventional linear and reflectron ion optical systems. This innovative ion optical system is developed by JEOL based on the “Perfect focusing*1” and “Multi-turn*2” principles developed at To detector Osaka University. The ion packets are focused back in space at every fixed distance (i.e., each figure-eight trajectory) during the flight. Thus, even after the extended flight distance, the ion packets do not diverge at the detection plane, achieving high mass resolving power, high mass accuracy,...

MALDI-TOFMS for the next generation Setting the new standard in MALDI-TOFMS performance JMS-3000 overcomes the limitation of the delayed extraction method. The JMS-3000 overcomes this challenge by extending the flight distance, thus successfully enhances the mass resolving power and mass accuracy. Ion source High Mass resolving power Mass accuracy Low 10m 15m The ion optical system of the MALDI-TOFMS consists of the delayed extraction ion optics (distance to the intermediate focal plane L1: red line) and energy focusing ion optics (distance L2: blue line). The delayed extraction method greatly...

Reduced topographic effect of matrix crystal The topographic effect of the matrix crystal leads to a dif- Acceleration direction ference of flight start position of ions, resulting in a differ- A difference of flight time arises due to the topographic effect of the matrix crystal. ence in flight time. In the conventional ion optical system, this time difference degrades the mass resolving power and also the mass accuracy obtained with external mass Matrix + sample calibration. With extended flight distance, the JMS-S3000 reduces this effect to the minimum and achieves highly reproducible mass...

TOF-TOF Option An ultimate structural analysis tool: high-energy CID of monoisotopically The JMS-S3000 with TOF-TOF option can acquire highenergy collision-induced dissociation (high-energy CID) product ion spectra of monoisotopically selected precur- 2nd TOF Offset Parabolic Reflectron sor ions. 2nd TOF Detector At the ion gate, precursor ions are selected from ion groups accelerated to 20 kV in the ion source. The Reacceleration Region 2nd Deflecting System distance to the ion gate is 15 m, more than one order of magnitude longer than that of conventional MALDI TOF-TOF instruments, thus allowing...

selected precursor ions [M+H]+ Asp-Arg-Val-Tyr-Ile-His-Pro-Phe-His-Leu Immonium Ions Val a2 da3 a6 a4 a3 Leu a5 Ile Tyr da5 a9 a8 His Pro Phe His a7 m/z Product ion spectrum of Angiotensin I. Fragmentation pathways originating from high-energy CID are predominantly observed with 20 keV collision energy and the removal of PSD ions by electric sectors in SpiralTOF. [M+Na]+ Precursor ion: m/z 1027 In the case of conventional TOF-TOF Enlarged spectrum Difficult to distinguish two different fragmentation pathways due to isotope peak overlap in the product ion spectrum. Na : m/z 23 * Simulated spectrum...

Analysis of Synthetic Polymer with SpiralTOF Example of copolymer analysis Sample : Poly(oxyethylene) and Poly(oxypropylene) Determination of repeating units and molecular mass distributions from the mass spectrum The mass spectrum is exported to Polymerix™ for analysis. Assignments | Filtered Assignments | Detail; \ Mass Ranee; flB Oistrfeution I Repeat ft Statistics | Repeat B Statistic; | Repeal ft Plot | Repeat E PloTT, Molar mass averages

Profile Spectrum Maximum peak: m/z 1027.7116, Height = 28292, Area = 159770, R = 57398 Profile Spectrum Maximum peak: m/z 1029.6910, Height = 8322. Area = 42992. R = 58462 Obtain structural information with MS/MS Product ion spectrum produced from mono-isotopic ions of (AO, B17)+Na.

Linear TOF Option Routine analysis of high molecular weight compounds The JMS-S3000 with Linear TOF option allows for high-sensitivity analyses of high molecular weight samples (molecular mass: several tens of thousands or more) and samples Linear TOF detector that tend to undergo post source decay. Alternative use of the Spiral and Linear modes enables a wider range of compounds to be analyzed efficiently. When operated in Linear mode, voltages are not applied to electric sectors. Ions flight linearly from the ion source to the detector. Linear TOF Ion Gate IgG Image 1+ 2+ 40000 80000 120000...