Solutions for your Life Science lab

Solutions for your Life Science lab

Solutions for your Life Science lab Life Science has experienced a great growth all around the world in the past decade. Scientists have made one breakthrough after another exploring the mysteries of nature. Studying the most basic mechanisms of life in organisms can be extended to treat diseases, to give more possibilities and new hope to patients. Those benefits for our world give a great meaning and depth to the field of Life Science, including all biological sciences exploring the structures of life. As the research gets deeper, the importance of sample preparations is rising, having a great...

PCR Sample Preparation PCR, or polymerase chain reaction, is a technique to amplify specific DNA sequences. The key to a qualitative and successful PCR is to minimize sources of error in the sample preparation process.

Challenge 1 Animal and plant tissues need to be disrupted at a low temperature to stabilize nucleic acids. IKA’s solution Tube Mill control The intermittent running mode helps to improve sample size uniformity and can be accompanied by dry ice, which avoids the thermal breakdown of DNA during high speed grinding. The main unit has a number of programs Tube Mill control installed, which can be altered by the user and several precautions for safe operations. Contamination-free processing can be achieved using single-use disposable milling chambers or autoclavable, reusable tubes. Technical Data...

ULTRA-TURRAX® Tube Drive P control Ident. No. 0025005981* T 10 basic ULTRA-TURRAX® Ident. No. 0003737000* * ubes are not included in the scope of delivery and have T to be ordered separately. * Dispersing tools are not included in the scope of delivery. ULTRA-TURRAX® Tube Drive P control The forward and reverse function can be used to help unwind Compact, hand-held tissue homogenizer for volumes from 0.5 tangled fibres when the sample is fiber-rich. The multi- to 100 ml with a quick-connect coupling for easy interchangea- pur ose device can be combined with tubes for different p bility of dispersing...

Challenge 2 Cell lysis with enzymes like Proteinase K requires temperature and time controlled incubation. IKA’s solution MATRIX thermoshaker A wide range of interchangeable attachments enables the thermoshakers to be flexibly adapted to different requirements and applications. Sample vessels with different volumes as well as microtiter plates and deep well plates can be mixed and tempered. Optionally, the insulated and heatable lid “ThermoCover” can be added to prevent temperature gradients in the vessel during incubation. Technical Data MATRIX Orbital MATRIX Orbital MATRIX Orbital MATRIX Orbital...

IKA’s solution To extract nucleic acids from the lysate, several purification steps need to be performed. IKA PETTE vario An ergonomic feel combined with uncompromisingly precise performance: the IKA PETTE vario can be used for many applications. The colour coding simplifies the rapid selection of a suitable pipette. The highly precise pipettes are fully autoclavable and resistant to UV-light and chemicals to ensure sterile working conditions and robust, long-lasting quality. Accidental volume changes can be avoided with the volume locking feature to ensure accurate pipetting results. Information...

* he scope of delivery includes the metal roT tor and cover for 12 vessels, with a capacity of 1.5 / 2 ml. * The scope of delivery includes rotors for 1.5 / 2 ml tubes and 0.2 ml PCR strips as well as several adapters for smaller tubes. IKA G-L is the ideal midi centrifuge for all types of sepa- Mini centrifuge for all applications which do not require ration processes such as the extraction of nucleic acids high speeds, like spinning down sample tubes, microfiltra- or for simple spin down applications. It can be used with tions or cell separation in tubes such as PCR vessels or PCR standard...

Molecular Cloning Molecular Cloning Molecular cloning is a method to produce many copies of a specific DNA fragment. The DNA or RNA is extracted from a sample and amplified in a PCR. The amplified fragment can then be digested with restriction enzymes and ligated with a vector to produce a plasmid with the desired DNA. A microorganism such as E.coli is then transformed with the plasmid, enriched and harvested to produce a high quantity of plasmids containing the desired DNA fragment.

Molecular Cloning Challenge 1 The extraction of nucleic acids is crucial for the success of the whole experiment. Therefore the sample has to be disrupted carefully and under low temperature conditions to prevent degradation of the DNA material. IKA’s solution Tube Mill control ULTRA-TURRAX® Tube Drive P control The sample can be mixed with dry ice directly during The disperser ensures the best stirring, dispersing, processing to avoid thermal degradation of DNA dur- grinding and homogenization results and has been ing high speed grinding (see page 5 for details). designed in particular for the...

Molecular Cloning Challenge 2 All enzyme reaction steps and the cultivation of E.coli require precise control of temperature and time to be able to successfully produce the plasmid. IKA’s solution MATRIX Mixing, heating and cooling functions are performed as required with precise temperature control. Uniform tempering of the samples is ensured by the various attachments fitting perfectly to the common reaction vessels in Life Science labs. The MATRIX thermoshakers are ideal for incubation of enzymatic reactions (see page 7 for details).

Molecular Cloning Dry Block Heater Digital dry bath incubator for aluminum blocks provides precise temperature control in small vessels. Included PT 1000.60 temperature sensor allows temperature control directly in the sample vessel. It can be used with PCR tubes, PCR strips, Greiner tubes, microplates and cuvettes. Technical Data Heat output Heating temperature range Adjustment and display resolution Fixed safety circuit Set-up plate material Aluminum alloy Time setting range