JOBIN YVON Eluorolog 3 September 2013 for printing.indd 1

How the Fluorolog® adapts to YOUR sample The Fluorolog® is the final concept in fluorescence engineering, an instrument that encourages you to custom-tailor a spectrofluorometer’s performance to the work you need to accomplish. Whether you use steady-state or molecular dynamics, your selections will deliver the perfect balance of these crucial benefits: Sensitivity Speed Modularity Automation Versatility Exclusivity Real-world performance The Fluorolog® delivers the ultimate in sensitivity. This means not only that you can see lower concentrations, but you also take data faster, which means more...

How we achieve the best sensitivity: Matrix scanning 1. ur CW xenon excitation lamps are mounted vertically to O image the arc on the slit for more throughput—with longer lamp-life as a bonus. Not only does the Fluorolog® software include routines for automatic scanning of emission spectra for a defined set of excitation spectra, to produce an excitation-emission matrix that fully characterizes the sample’s fluorescence, the monochromator’s unique design supplies fast scanning (150 nm/s) to make these scans practical. Your samples can be totally characterized in a matter of minutes, as shown...

Choose the components you need to maximize the sensitivity, speed, wavelength, timing, sample-handling, or other important parameters. pulsed lamp Your laser Optional accessories Sources 450 W xenon CW lamp is standard. Options include a pulsed xenon lamp for phosphorimetry, a laser port for your own laser source, NanoLED solid-state pulsed sources, a triple-illuminator option to mount nanosecond or microsecond flash-lamps, and more. Excitation Choose a single-grating unit with kinematic gratings to customize your spectral range, or a double- monochromator grating unit for highly scattering samples....

Recommended Modular Configurations FL3-11 The basic Fluorolog® configuration is formed from single-grating monochromators in excitation and emission positions, a T-sample compartment, and a redsensitive photomultiplier. Add any accessory now, or expand your capabilities later. The FL3-11 provides outstanding sensitivity and performance at the lowest price. Switch from steady-state measurement to picosecond lifetimes with the optional MF2 automated system as easily as clicking on a mouse, without any realignment. MF2 is the fastest, most sophisticated system for molecular dynamics as you probe...

Versatility Fluorolog® Accessories Fiber-optic platform F-3000 Use this accessory for remote-sensing from 250– 850 nm for samples that cannot be placed in the sample chamber. Liquid-nitrogen Dewar FL-1013 To measure phosphorescence or delayed fluorescence, samples are often frozen at liquid-nitrogen temperature to preserve the fragile triplet state. A Dewar flask is used to freeze and maintain the temperature of the sample. The sample is placed in a quartz cell, and slowly immersed in the liquidnitrogen-filled Dewar. The Dewar is on a pedestal within the Fluorolog®’s sample compartment. Automated...

Thermoelectric heater/ cooler F-3004 Stopped-flow Accessory For heating and cooling samples without external circulating baths. You can rapidly heat and cool your fluorescent material through a wide range of temperatures using the Peltier effect. A magnetic stirrer is included. The stopped-flow accessory adds the dimension of kinetics research to your instrument, perfect for analyzing fluorescence reactions on the millisecond time-scale. More accessories for the Fluorolog®: Model Standard-lamp correction factor kit Thermoelectrically cooled R928P photomultiplier tube 4 mL quartz cuvette with...

Real-World Performance Whether you’re working in biochemistry or nanomaterials, measuring calcium-migration, intermolecular distances, or laser crystals, the sensitivity and flexibility of a Fluorolog® spectrofluorometers will help you gather more information on more samples in a smaller amount of time. When the focus of your research changes, so can the Fluorolog®, adapting modularly to the demands of your work with upgrades and innovations. Here are just a few examples. Detecting fluorescence in highly scattering samples With highly scattering samples, fluorescence signals may be overwhelmed...

Synchronous scanning for characterizing complex mixtures The observed fluorescence spectrum of a complex mixture often contains overlapping spectral features. Synchronous scanning offers a solution to this problem by simultaneously scanning the excitation and emission monochromators with a constant offset between them (in units of wavelength or wavenumbers). provided by a variety of solid-state detectors, covering different spectral regions, is available, as are choppers and lock-in amplifiers for enhanced sensitivity. Only a Spex® Fluorolog® IR system includes these components as integrated...

IIHIWn Performance, continued Fluorescence from the singlet state usually occurs within a few nanoseconds after excitation. Because triplet transitions are more inhibited, the average phosphorescence-decay times are longer, ranging from microseconds to seconds, offering ,a longer observation period for monitoring reactions, viewing effects of the local molecular environment on a sample, or following changes in the hydrodynamic characteristics of macromolecular systems. Jn phosphorescence experiments, the Fluorolog® with the :FL-1040 dual-lamp housing—which includes a pulsed light I source—can...

Front-face detection for absorbent or solid sample Fluorescence is typically collected at 90° to the excitation beam to minimize interference from scattered light. Yet rightangle viewing is not feasible with some samples. Imprint paper, for example, should not be viewed at 90° because of interference with reflected light. In highly absorbent samples like hemoglobin or milk, most of the emitted light is reabsorbed internally before the fluorescence can be measured. Detecting trace quantities of biological probes with fluorescence polarization A significant optional feature of the Fluorolog® is...