HORIBA Scientific TWO tlA/ OM\ Explore the future Automotive Test Systems I Process & Environmental I Medical I Semiconductor I Scientific HORIBA

A Superior Spectrometer Concept • Two-in-one Fluorescence and Absorbance Spectrometer • UV-Vis-NIR Fluorescence Detection Wavelength Range from 250 to 1,100 nm • Full 3-D Fluorescence EEM Acquisition in Less Than One Second • Best in Class Fluorescence Sensitivity Specification of 6,000:1 RMS for Water Raman • Automatic Correction for Primary and Secondary Inner Filter Effects (IFE) • High Fidelity Molecular Fingerprinting with Unique A-TEEM™ (Absorbance-Transmittance Excitation Emission Matrix) Technology • Millisecond CCD Detection of Entire Fluorescence Spectrum Fluorescence and Absorbance...

A New Spectrometer Design Ergonomic, Hands Free, Sample Access Fluorescence and Absorbance Duetta’s two specialized sample detectors collect fluorescence at right angles to the excitation light, and absorbance straight through the sample. Normalized Intensity Integrated FL Intensity (rel. units) Absolute Fluorescence Spectra with Real Time Inner Filter Effect (IFE) Correction IFE limits the linearity of the fluorescence signal at higher sample concentrations due to primary and secondary re-absorption of fluorescence. Duetta’s unique design applies real time IFE correction to obtain absolute fluorescence...

Intensity (Counts/µA) Chemistry Life Sciences Materials Research Food Science Earth Sciences Intensity (Counts/µA)

Intensity (Counts/µA) Intensity (Counts/µA) Peak Emission Intensity vs. Temperature Pyrene in a solution of A-B-A triblock copolymer micelles is solvated in the hydrophobic core solvent environment. 3D EEMs can be shown in contour, waterfall plot, or 2D overlay mode. BSA protein measured from 5 °C to 60 °C and the peak intensity and peak wavelength are plotted vs. temperature of the protein solution. Measured using the SampleSnap-4Pelt 4-position Peltier temperature-controlled cuvette holder. Molecular Binding (FRET) Intensity (Counts/µA) Polymer Aggregation and Structure. Temperature dependent...

EzSpec Software Apps at your Fingertips From the EzSpec™ launch screen, each App icon intuitively helps you identify and select the analysis you want to perform with a simple point and touch. Widest Range of Spectroscopic Acquisition Modes: • Fluorescence kinetics (emission spectra*lor single point intensities) • Absorbance and % Transmittance (spectra or kinetics) HORIBA izSpec METHOD SETUP Autonomous Acquisition Build/Use a concentration curve 1L Emission Spectra Excitation Spectra Capture Value Standard Apps: • Autonomous Acquisition • Pass/Fail • Build/Use a Concentration Curve • Protein...

Accessories Optional Sample Holder Trays SampleSnap-WJ: Thermostated and Micro-Stirred Cuvette Sample Tray Self-identifying thermostatable, water jacketed cuvette holder (1 cm) with magnetic micro-stir motor. The sample tray can be installed in seconds without the need of a tool, and provides power for micro-stirrer. Requires, but does not include, external water bath. SampleSnap-1Pelt: Single Cuvette Peltier Holder Sample Tray Self-identifying single cuvette Peltier holder (1 cm) for automated temperature control and ramping from -15 °C to +105 °C, with magnetic micro-stirrer. Sample tray can...

PW EEMs QDot LRET GFP FLIM Our Roots Grow Deep HORIBA has a long history of global leadership in high performance fluorescence research instrumentation. Now, with the introduction of Duetta, HORIBA has perfected an affordable spectrofluorometer that takes advantage of decades of experience in fluorescence. (Think SPEX, Jobin Yvon, SLM, IBH, PTI and HORIBA). HDRIBn.com/Duetta 2008 HORIBA HORIBA Scentifc 1988 SPEX purchased by Jobin Photon Technology International acquired by HORIBA Scientifc http://www.horiba.com e-mail: [email protected] HORIBA, Ltd.Japan Head Office 2 Miyanohigashi,...