DeltaTime Add a new dimension to your fluorescence Add the power of fluorescence lifetime spectroscopy to our steady-state fluorometers ELEMENTAL ANALYSIS FLUORESCENCE FLUORESCENCE GRATINGS & OEM SPECTROMETERS OPTICAL COMPONENTS FORENSICS PARTICLE CHARACTERIZATION RAMAN SPECTROSCOPIC ELLIPSOMETRY SPR IMAGING

Why measure fluorescence lifetimes? Fluorescence is an ideal nanoscale probe, as it takes place on the nanosecond timescale and can be influenced by molecular processes occurring on the nanometer range. The emission lifetime of a fluorophore can be highly influenced by its environment or the presence of other interacting molecules. Thus fluorescence lifetime is useful in measuring: • Changes in the nanoenvironment (viscosity, pH, polarity, solvation) • Size and shape of molecules • Molecular interactions • Inter- and intramolecular distances • Kinetic and dynamic rates • Resolution of molecular...

DeltaTime Make the most sensitive and flexible fluorometers even more versatile by adding lifetime capability The DeltaTime TCSPC lifetime plug-in offers acquisition speed, flexibility, and affordability unavailable in any other hybrid fluorescence solution available. DeltaTime seamlessly integrates monochromators, polarizers, and other accessories with the widest array of sources (LEDs, laser diodes, supercontinuum lasers) and detectors (including NIR), providing lifetime coverage from 25ps to 1sec over wavelengths spanning the UV to NIR. The culmination of over 40 years of lifetime experience,...

Measurement types and examples The Delta series include configurations ideal for all modes of lifetime measurements, including kinetics, anisotropy and TRES (time resolved emission spectra), for all lifetime applications such as FRET, measurement of physical constants (such as local viscosity), binding studies and photophysical characterization of molecular interactions. • acquire data in as little as 1 ms • molecular interactions • understanding photophysical processes Kinetic TCSPC • collect up to 10,000 decays sequentially • acquisition times from 1 ms to 1 minute • kinetic studies • binding...

Measurement types and examples (continued) Anisotropy • fluorescence and phosphorescence timescales • reconvolution to determine short correlation times Useful for… • molecular interactions • changes in local viscosity • determinations of molecular size • energy depolarization processes Time-resolved emission spectra (TRES)* • • • • • create intensity, wavelength, time surfaces fluorescence and phosphorescence timescales time slice spectral data global analysis of up to 100 wavelength dependent decays enables determination of decay associated spectra Useful for… • resolving spectra from a mixture...

Simple yet powerful acquisition and analysis software Allows control over the hardware and data collection, plus a complete suite of data analysis modules making use of our proprietary hybrid search algorithm. Analysis for up to 5 exponentials Exciplex kinetics Lifetime distribution Top-hat Non-extensive decay (NED) Exponential series Förster energy transfer Yokota-Tanimoto energy transfer Micellar quenching Anisotropy analysis (including reconvolution to resolve short correlation times) Batch exponential analysis Global exponential analysis These modules include our proprietary NED distribution...

DeltaTime components: DeltaTime excitation sources: From UV to NIR , all excitation sources are software controlled, with the repetition rate automatically adjusted to suit the time range. Utilize laser diode and LED technology to generate short optical pulses over a very wide range of repetition rates and wavelengths. Features: Optical pulses as short as 40ps for laser diode based sources Repetition rates up to 100MHz (20MHz for LEDs) Wavelengths 250-1310nm (See website for full range and specifications) CW available on most laser diode versions Plug-n-play compatible within DeltaDiode range...

DeltaTime detectors DeltaTime can use existing PMT detectors included in the steady-state system, or, in the case of the Fluorolog modular series, also detectors optimized for lifetime measurements. Our PPD detectors are compact and include all electronics required to detect single photons with picosecond accuracy. The module contains a wide bandwidth GHz pre-amplifier, picosecond timing constant fraction discriminator and regulated high voltage supply all in one compact, fully integrated package. NIR detectors are also available. These can extend the wavelength range up to 1700 nm. For detectors...