DeltaPro DeltaFlex ELEMENTAL ANALYSIS FLUORESCENCE FLUORESCENCE GRATINGS & OEM SPECTROMETERS OPTICAL COMPONENTS PARTICLE CHARACTERIZATION RAMAN SPECTROSCOPIC ELLIPSOMETRY SPR IMAGING

Why measure fluorescence lifetimes? Fluorescence is an ideal nanoscale probe, as it take places on the nanosecond timescale and can be influenced by molecular processes occurring on the nanometer range. The emission lifetime of a fluorophore can be highly influenced by its environment or the presence of other interacting molecules. Thus fluorescence lifetime is useful in measuring: • Changes in the nanoenvironment (viscosity, pH, polarity, solvation) • Size and shape of molecules • Molecular interactions • Inter- and intramolecular distances • Kinetic and dynamic rates • Resolution of molecular...

The Delta Series From the simplest, easiest to use TCSPC system to the most flexible and versatile The new Delta series of fluorescence TCSPC lifetime systems are the fastest, most flexible, and most affordable lifetime solutions available. The Delta series includes the DeltaPro, the very affordable and simple to use filter based lifetime system with performance that rivals most high end systems. For the ultimate flexibility and upgradability, DeltaFlex, our modular system, seamlessly integrates monochromators, polarizers, the widest array of sources (LEDs, laser diodes, supercontinuum lasers)...

DeltaProTM The DeltaPro is a remarkably affordable, easy to use filter-based lifetime system that still offers capabilities found in far more expensive and complex systems. DeltaPro’s simplicity makes it the ideal system for a multiuser facility with researchers who need to measure lifetimes or a combined research/ teaching lab. But don’t let the price and approachability fool you. The DeltaPro is a serious research instrument capable of picosecond lifetime measurements. With addition of SpectraLED excitation sources it instantly becomes a phosphorimeter. Our proprietary optimized performance...

Measurement types and examples The Delta series include configurations ideal for all modes of lifetime measurements; including kinetics, anisotropy and TRES (time resolved emission spectra), for all lifetime applications such as FRET, measurement of physical constants (such as local viscosity), binding studies and photophysical characterization of molecular interactions. Lifetime • from 25ps to 1s • acquire data in as little as 1ms Useful for… • molecular interactions • LRET / FRET • determination of paramters • understanding photophysical processes Kinetic TCSPC • collect up to 10,000 decays...

Measurement types and examples (continued) Anisotropy • fluorescence and phosphorescence timescales • reconvolution to determine short correlation times Useful for… • molecular interactions • changes in local viscosity • determinations of molecular size • energy depolarisation processes Time-resolved emission spectra (TRES)* • • • • • create intensity, wavelength, time surfaces fluorescence and phosporescence timescales time slice spectral data global analysis of up to 100 wavelength dependent decays enables determination of decay associated spectra Useful for… • resolving spectra from a mixture...

Delta series components: Delta series excitation sources: From UV to NIR , all excitation sources are software controlled, with the repetition rate automatically adjusted to suit the time range. Utilize laser diode and LED technology to generate short optical pulses over a very wide range of repetition rates and wavelengths. Features: Optical pulses as short as 40ps for laser diode based sources Repetition rates up to 100MHz (20MHz for LEDs) Wavelengths 250-1310nm (See website for full range and specifications) CW available on most laser diode versions Plug-n-play compatible within DeltaDiode...

Delta series detectors PPD detectors are compact and include all electronics required to detect single photons with picosecond accuracy. The module contains a wide bandwidth GHz pre-amplifier, picosecond timing constant fraction discriminator and regulated high voltage supply all in one compact, fully integrated package. NIR detectors are also available. These can extend the wavelength range up to 1700nm. For detectors whose wavelength response starts at 950nm, it is also possible to couple both a NIR detector and PPD to the same monochromator using a selectable mirror block. DeltaHub timing...

Gives excellent stray light rejection and a time dispersion of 0.13ps/nm. They are designed with a Seya-Namioka geometry and feature integral safety shutter interlocked to the sample compartment and computer controlled adjustable slits and wavelength drive. Sample holders • Standard with stirrer and temperature sensor • Front face, able to accept cuvettes for concentrated samples • Front surface for use with solid samples Temperature control and 4 position turret optional

Simple yet powerful acquisition and analysis software Allows control over the hardware and data collection plus a complete suite of data analysis modules making use of our propriety hybrid search algorithm Analysis for up to 5 exponentials Exciplex kinetics Lifetime distribution Top-hat Non-extensive decay (NED) Exponential series Förster energy transfer Yokota-Tanimoto energy transfer Micellar quenching Anisotropy analysis (including reconvolution to resolve short correlation times) Batch exponential analysis Global exponential analysis These modules include our propriety NED distribution analysis....

DeltaPro Specifications 25 ps with laser-diode source • 30 ps with laser-diode source Minimum lifetime Shortest acquisition time Diode controller Repetition rates Prompt FWHM Dead time <200 ps with PPD and laser diode Time ranges Wavelength selection Interchangeable filters (filters purchased separately from HORIBA or others) Detector response USB 2.0. PC not included. Requires Windows® XP or Windows® 7, 32/64-bit 75 cm x 45 cm nominal excluding PC (depending on options) System footprint DeltaFlex Specifications Minimum lifetime Shortest acquisition time 25 ps with laser-diode source* Diode controller...