RAMOS Bioprocess optimization in shake flasks

Product catalog summary

Overview of RAMOS® System
The RAMOS® (Respiration Activity Monitoring System) is a universal measuring system designed for the online determination of respiratory activities in prokaryotic and eukaryotic cultures within shake flasks. It supports process development by providing critical metabolic parameters such as Oxygen Transfer Rate (OTR), CO2 Transfer Rate (CTR), Respiratory Quotient (RQ), and maximum specific growth rate (μ_max).

Key Features

Enables 8-fold parallel online measurement under sterile conditions in 250 ml and 500 ml flasks.
Temperature control ranging from +30°C to +50°C, with a cooling system 5°C below room temperature.
Determination of OTR, CTR, RQ, μ_max, and kLa.
Fed-Batch operation with a feeding system.
Control of gas phase (O2, CO2) with the Gmix™ gas mixing station.
Phototrophic cultivation with the CultiLux™ exposure module.

Applications

Process development and optimization, including early detection of oxygen and substrate limitations.
Media optimization for substrate affinity investigation.
Stem screening and optimization, such as enzyme production for biofuels.
Fermentation validation and stability testing.
Toxicity and degradation studies, e.g., wastewater treatment.
Process balancing and quantification, including carbon accounting.
Determination of kinetic growth and process parameters.

Research and References

RAMOS® has been used in various studies to optimize media for osmotic tolerant yeasts and to quantify phototrophic growth in organisms like Arthrospira platensis.
It has also been applied in plant cell culture screening, demonstrating its versatility in different biological research areas.

Contact Information
HiTec Zang GmbH, Ebertstraße 28-32, 52134 Herzogenrath, Germany. Tel: +49 (0)2407 / 910 100, Fax: +49 (0)2407 / 910 1099, Email: [email protected], Website: www.hitec-zang.de

Catalog excerpts

RAMOS Bioprocess optimization in shake flasks-1

Features The RAMOS® enables an 8-fold parallel online measurement of respiratory activities and process control under sterile conditions in 250 ml and 500 ml flasks. Temperature control (+30…+50 °C, with cooling system 5 °C below RT) Determination of OTR, CTR, RQ and µmax kLa determination Fed-Batch with feeding system Control gas phase (O2, CO2) in combination with gas mixing station Gmix™ Phototrophic cultivation in combination with exposure module CultiLux™ Process development and optimization (early detection of oxygen and substrate limitation or product inhibition) Media optimization (investigation of substrate affinity) Stem screening and optimization (e. g. enzyme production for biofuels) Fermentation validation Stability testing and quality control (e. g. stability of mammalian cell lines) Toxicity studies and degradation studies (e. g. wastewater treatment) Process balancing and quantification (e. g. carbon accounting) Determination of kinetic growth and process parameters (µmax, Ks, kLa) References [1] R. Krüger, "Praktikumsversuch RAMOS®", HiTec Zang GmbH, 2005 [2] J. Pfannebecker, C. Schiffer-Hetz, J. Fröhlich, B. Becker, "Culture medium optimization for osmotolerant yeasts by use of a parallel fermenter system and rapid microbiological testing", Journal of Microbiological Methods, vol. 130, pp. 14-22, 2016 [3] M.L. Socher, F. Lenk, K. Geipel, C. Schott, J. Püschel, C. Haas, C. Grasse, T. Bley, J. Steingroewer, "Phototrophic growth of Arthrospira platensis in a respiration activity monitoring system for shake flasks (RAMOS®)", Engineering in Life Sciences, vol. 00, pp. 1-9, 2014 [4] K. Geipel, M.L. Socher, C. Haas, T. Bley, J. Steingroewer, "Growth kinetics of a Helianthus annuus and a Salvia fruticosa suspension cell line: Shake flask cultivations with online monitoring system", Engineering in Life Sciences, vol. 13, pp. 593-602, 2013 HiTec Zang GmbH Ebertstraße 28-32 52134 Herzogenrath

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RAMOS Bioprocess optimization in shake flasks-2

Respiration Activity MOnitoring System Bioprocess optimization in shake flasks

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RAMOS Bioprocess optimization in shake flasks-3

Universal measuring system RAMOS® (Respiration Activity MOnitoring System) has established itself worldwide as the standard for online determination of the respiratory activity of proand eukaryotic cultures in shake flasks. In numerous processes investigated so far RAMOS® has helped to gain key knowledge and has given the decisive impulse for development work which in some cases took many years. RAMOS® provides the really meaningful metabolic parameters O2- (OTR) and CO2-transfer rate (CTR), as well as the derived parameters respiratory quotient (RQ) and the maximum specific growth rate (μmax)....

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RAMOS Bioprocess optimization in shake flasks-4

1. Cellular Physiological Phenomena unlimitiert Polyauxie Substratinhib. Reaction time Reaction time

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