BIREFRINGENCE MEASUREMENT PRODUCT BULLETIN Hinds Instruments has applied the power and versatility of the Photoelastic Modulator (PEM) to produce a birefringence imaging microscope. As the world leader in PEM technology, Hinds has manufactured and developed PEMs for over 50 years. For more than 20 years Hinds has built birefringence measurement solutions, marketed under the Exicor® brand. The superior polarization modulation capabilities of the PEM have delivered the highest sensitivity birefringence measurement solutions on the market. Birefringence Imaging Microscope The Exicor Birefringence MicroImager offers researchers in academia and industry the ability to evaluate birefringence of both biological and industrial materials. With a spatial resolution down to 0.7μm, a detection limit (noise floor) of Figure 1. Myelinated White Matter in a mouse brain. 2nm and a measurement range of beyond 3500nm, the MicroImager offers quick and reliable measurements in an integrated system package that includes camera, optics, electronics, sample stage hardware and software. The system offers a choice of 3 or 4 Color configurations: red, orange, green or blue light sources, and a choice of either a 2X (5 micron resolution) or 5X (2 micron resolution) objective. Additional objectives may be added as options, including 2X, 5X, 10X and 20X objectives. The MicroImager turret allows mounting of up to 4 objectives. The Exicor MicroImager system icomes standard with a phase unwrapping solution for determination of magnitude of high birefringence samples (i.e. >300nm). The phase unwrapping software uses a proprietary algorithm to estimate the true retardation magnitude (up to 2400nm for RGB systems and 3500nm+ for ROGB systems). The system can also be customized to use other wavelength sources (contact Hinds for details). The Hinds Birefringence Imaging Microscope is ideal for measuring birefringence in biological structures (see Figure 1), glass, crystals, and many other organic and inorganic samples. Figure 2. The Exicor Birefringence MicroImager. Now with customer requested turret. Technology for Polarization Measurement

BIREFRINGENCE MEASUREMENT PRODUCT BULLETIN FEATURES No Dyes or Fluorescent labels required Measures retardation, angle and intensity Images retardation, angle and intensity Allows color maps to be customized by user for optimal display of data Allows maximum, average, and standard deviation over entire image and in user-selected areas or lines Dandelion Fuzz 10x retardation and angle Ranunculus Root, c.s. 10x Retardance Silver berry scaly hair 10x Retardance Dog Colurmar Epihelium 10x Retardance MULTI TAPE SAMPLE RETADANCE View data by intensity, birefringence/retardation, angle, or combination...

BIREFRINGENCE MEASUREMENT PRODUCT BULLETIN RESOLUTION AND FIELD OF VIEW Red (655nm) Orange (615nm) Green (530nm) Blue (475nm) Retardation Measurement Range unwrapping to 3500nm Measurement Speed Dimensions Magnitude above 2nm Phase Unwrapping: 3 color system to 2400nm, 4 color system to 3500nm 3 2X, 5X, 10X Noise Floor ~1nm. 20X Noise Floor ~2nm 4 20X includes a lens to focus beam on the detector                                                                                                   ...