Certified Reference Materials for UV/ Vis Spectroscopy PRODUCT CATALOG WITH USAGE GUIDELINES GLASS FILTERS LIQUID FILTERS REFERENCE PLATES TRACEABLE

YOUR PROCESS. OUR SOLUTIONS. As a leading supplier of high-precision, optical solutions that are 'Made in Germany' from glass, quartz glass and synthetic crystals, Hellma has been a by-word for outstanding quality for over 95 years. A key supplier, the company is an integral part of its clients’ value chains. Reliability, trust and continuity are inherent in Hellma’s work, and the company believes it has both a duty and responsibility to ensure these principles are upheld. Clients from more than 40 countries worldwide put their confidence in Hellma’s exceptional level of performance and problem-solving...

1.1 Hellma Analytics calibration laboratory Page 7 2.1 Checking wavelength accuracy 2.1.1 Holmium glass filter 2.1.2 Didymium glass filter 2.2 Checking the photometric accuracy 2.2.1 Didymium glass filter F7A 2.2.2 Neutral density glass filter 2.3 Glass filter sets 2.4 General usage guidelines for glass filters 2.5 Calibration with glass filters 2.5.2 Steps for checking wavelength accuracy with holmium glass or didymium glass filter 2.5.3 Steps for checking photometric accuracy with a Neutral density glass filter or a didymium glass filter (F7A) 2.5.4 Calibration...

1.1  Hellma Analytics calibration laboratory: accredited to DIN EN ISO 17025 Our lab is a DAkkS calibration laboratory and is accredited to DIN EN ISO 17025, a comprehensive quality management system that acts as a seamless continuation of other systems such as ISO 9001. By achieving this accreditation, we have demonstrated proof of expertise in the calibration activities that we perform and are authorized to issue internationally recognized DAkkS calibration certificates. The accreditation is the key to high quality measurements, international comparability, and trust in both the work of the...

1. INTRODUCTION 1.2 Certified test equipment Quality assurance and quality control regulations, such as ISO 9001, GLP, GMP, and Pharmacopeias, require companies to verify the consistently excellent performance of any spectrophotometer in use. The two most important factors for obtaining precise spectrophotometer data are the photometric accuracy (absorbance accuracy) and wavelength accuracy of the spectrophotometer, which should be tested on a regular basis. In the Hellma Analytics calibration laboratory, which is accredited to DIN EN ISO 17025, we manufacture certified reference materials based...

1.5 DAkkS calibration certificate After careful production, reference materials are certified in the Hellma Analytics calibration laboratory (accredited to DIN EN ISO 17025) using a high-performance UV-Vis/NIR spectrophotometer. Reference materials are only consid ered to be certified if they have been issued with a DAkkS calibration certificate and bear a calibration mark. Using the measurement values documented and certified in the calibration certificate, users can check and calibrate their spectrophotometers accordingly. IMPORTANT INFORMATION Only if the DAkkS calibration certificate has...

1. INTRODUCTION 1.5 DAkkS calibration certificate lD 1.6 Warranty 30-year manufacturer's warranty on all Hellma Analytics reference materials Measurement value and smallest attributed measurement uncertainty that can be specified. This value only refers to Hellma Analytics measurements and applies solely to the company's specific measurement conditions. In justified cases, calibration certificates may also show measurement results that do not fall within the calibration laboratory's scope of accreditation. These must be clearly labeled as such on the calibration certificate. Notes on initial...

GLASS FILTERS 2. GLASS FILTERSWAVELENGTH ACCURACY 2.1 Checking wavelength accuracy APPLICATION To measure wavelength accuracy, the filter absorbs the light beam of the spectrophotometer to a greater extent at certain wavelengths creating absorbance peaks. Ideally, any reference materials used to determine wavelength accuracy should have narrow, well-defined peaks at a variety of wavelengths in the UV and visible range. 2.1.1 Holmium glass filter PRODUCT DESCRIPTION The holmium glass filter 666-F1 has a range of narrow, well-defined peaks in the UV and visible range, making holmium an excellent...

GLASS FILTERS 2. LASS FILTERS G PHOTOMETRIC ACCURACY 2.2  Checking the photometric accuracy APPLICATION To measure photometric accuracy (absorbance), the filter reduces the light beam from the spectrophotometer. An absorbance value (Abs) can be deduced from the light extinction caused by the filter. Absorbance behavior in the UV range can be checked at 270 nm, 280 nm, 297 nm, 321 nm, and 342 nm. Filters are routinely set at a thickness that produces a nominal optical density of 0.5 Abs at 342 nm. This results in increasingly larger absorbances the shorter the wavelengths become. 2.2.1  Didymium...

2. LASS FILTERS G SETS 2.3  Glass filter sets 2.4  General usage guidelines for glass filters Specifically created to meet customer requirements, Hellma Analytics glass filter sets consist of existing individual filters suitable for standard or custom validation procedures. Glass filters are made of glass doped with metal ions/rare earth metals, which are assembled stress-free in black anodized precision frames made of aluminum. They are designed to fit into all spectrophotometers equipped with a holder for standard cuvettes with a 10-mm optical path length. To ensure that filters can be easily...

2.5  Calibration with glass filters 2.5.1  Preparations 2.5.2  Steps for checking wavelength accuracy with holmium glass or didymium glass filter 1 First, carry out the "Preparations" according to chap. 2.5.1 Warm up the spectrophotometer until the correct operating temperature has been reached and remains constant (e.g. for one hour), taking care to observe the device manufacturer’s guidelines. Run the scan program on your spectrophotometer, observing the guidelines in the user manual. Select a scanning range that covers all of the peaks listed on the filter’s calibration certificate. Make sure...