// EASY HANDLING // RELIABLE MEASUREMENT RESULTS // INTERNATIONAL COMPARABILITY Certified UV/Vis Reference Materials GUIDELINES // GLASS FILTERS // LIQUID FILTERS // CERTIFIED REFERENCE PLATES

1.3 Glass filter applications 1.4 Liquid filter applications 1.5 DAkkS calibration certificate 1.6 Expanded absorbance range - from 0 to < 3.1 for UV-Vis reference materials ®Iglass filters 2.1 Holmium oxide glass filter for checking wavelength accuracy 2.2 Didymium glass filter for checking wavelength accuracy 2.3 Didymium glass filter for checking photometric accuracy 2.4 Neutral density glass filters for checking photometric accuracy 2.5 Glass filter sets 2.6 General usage guidelines for glass filters 2.7 Calibration with glass filters ®|LIQUm FILTERS 3.1 Holmium oxide liquid filter for checking...

Dear Readers, Although checking measuring equipment to ensure that results are accurate has long been common practice for analytical balances, it still tends to take something of a backseat where spectrophotometers are concerned. Spectrophotometers are important instruments that play a major role in health care, the life sciences, environmental analysis and processes such as production control and ensuring product quality. Over the last two years, many laboratories have become considerably more aware of the need to check their spectrophotometers, making it all the more important to know that...

Certified test equipment Quality assurance and quality control regulations, such as ISO 9000, GLP, GMP, and pharmacopoeias, require companies to verify the consistently excellent performance of any spectrophotometer in use. The two most important factors for obtaining precise spectrophotometer data are the photometric accuracy (absorbance accuracy) and wavelength accuracy of the spectrophotometer, which should be tested on a regular basis. In the Hellma Analytics calibration laboratory, which is accredited to DIN EN ISO 17025, we manufacture certified reference materials based on the regulatory...

HELLMA ANALYTICS REFERENCE MATERIALS IN ACCORDANCE WITH THE MOST IMPORTANT SET OF RULES AND REGULATIONS Holmium oxide glass Sodium iodide solution Stray light Sodium nitrite solution Stray light

DAkkS calibration certificate After careful production, reference materials are certified in the Hellma Analytics calibration laboratory (accredited to DIN EN ISO 17025) using a high-performance UV-Vis/NIR spectrophotometer. Reference materials are only conside- red to be certified if they have been issued with a DAkkS calibration certificate and bear a calibration mark. Using the measurement values documented and certified in the calibration certificate, users can check and calibrate their spectrophotometers accordingly. T ACCURATE MEASUREMEN RESULTS GUARANTEED. Name of accreditation body 2...

2014-12 Kalibriergegenstand: Kalibrierstandard-Satz, bestehend aus drei Neutralglas-filtern NG11, NG5 und NG4. Calibration Object: Set of calibration filters, consisting of three neutral density glass filters NG11, NG5 and NG4. Kalibrierverfahren: Messung der optischen Dichte. Diese Kalibrierstandards wurden gegen Luft als Referenz gemessen. Calibration Method: Measurement of optical density. These calibration standards were measured using air as reference. Messtechnische Bedingungen bei der Kalibrierung: Die in diesem Kalibrierschein angegebenen Werte wurden mit dem verwendeten Spektralphotometer...

Measurement value and smallest attributed measurement uncertainty that can be specified. This value only refers to Hellma Analytics measurements and applies solely to the company’s specific measurement conditions. In justified cases, calibration certificates may also show measurement results that do not fall within the calibration laboratory’s scope of accreditation. These must be clearly labeled as such on the calibration certificate. Notes on initial measurements of filters used to determine optical density. Initial measurements are not taken for filters used to determine wavelength accuracy....

Extended Abs.range till < Extended absorption range – from 0 to < 3.1 for UV/Vis reference materials Under the previous accreditation, the company was certified to manufacture UV-Vis reference materials for wavelength accuracy and optical density up to an absorbance of 2.05 A. However, pharmaceutical applications, biotechnology and spectrophotometer manufacturers all increasingly require reference materials with a certified absorbance up to 3 A. Since NIST (National Institute of Standards and Technology) is no longer producing the relevant SRM 2930 filter set, Hellma Analytics has manufactured...

GLASS FILTERS WAVELENGTH ACCURACY Holmium oxide glass filter for checking wavelength accuracy APPLICATION To measure wavelength accuracy, the filter absorbs the light beam of the spectrophotometer to a greater extent at c ­ ertain wavelengths creating absorbance peaks. Ideally, any reference materials used to determine wavelength accuracy should have narrow, well-defined peaks at a variety of wavelengths in the UV and visible range. NOTES The positions of holmium oxide peaks may vary slightly depending on the glass batch used. This is why Hellma Analytics certifies each holmium oxide glass filter...

Didymium glass filter for checking wavelength accuracy NOTES The positions of didymium glass peaks may vary slightly depending on the glass batch used. This is why Hellma Analytics certifies each didymium glass filter individually. APPLICATION Please see 2.1 for more details of the application. PRODUCT DESCRIPTION Didymium glass filter 666-F7W is made from material specially manufactured by Schott AG. Like holmium oxide glass, didymium glass has a variety of characteristic peaks in the ultraviolet and visible range and is therefore typically used for checking wavelength accuracy. Its peaks are...

GLASS FILTERS PHOTOMETRIC ACCURACY Didymium glass filter for checking photometric accuracy APPLICATION To measure photometric accuracy (absorbance), the filter r ­ educes the light beam from the spectrophotometer. An absorbance value (Abs) can be deduced from the light extinction caused by the filter. Absorbance behavior in the UV range can be checked at 270 nm, 280 nm, 297 nm, 320 nm, and 340 nm. Filters are routinely set at a thickness that produces a nominal optical density of 0.5 Abs at 340 nm. This results in increasingly larger absorbances the shorter the wavelengths become. PRODUCT DESCRIPTION...