Warner Instruments Ussing Chamber Systems Guide
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Ussing Chamber A Harvard Apparatus Company

Ussing System Introduction 1-2 Warner Classic Ussing Systems U9500 & U9520 3-5 Warner Self-Contained Ussing Chambers U9926 & U2500 6-7 Introduction to NaviCyte & EasyMount Chamber Systems 8 NaviCyte Horizontal 9 - 10 NaviCyte Vertical 11 - 15 EasyMount 16 - 18 Epithelial Voltage Clamps 19 - 20 Single Channel Epithelial Voltage Clamps 21 Multi Channel Epithelial Voltage Clamps 22 - 23 Input Manifolds & Dummy Membranes 24 Aquire & Analyze Data Acquisition 25 Data Acquisition and Analysis System HAI-118 26 - 28 Computer Controlled Multi-Clamp with Software 29 U9926 Single Channel Chamber and...

Ussing Chambers Systems From Warner An Ussing System, used to measure transport across epithelial membranes, is generally comprised of a chamber and perfusion system, and, if needed, an amplifier and data acquisition system. The heart of the system lies in the chamber with the other components performing supporting roles. The 'Classic' chamber design, first introduced by the Danish physiologist Hans Ussing in the early 1950's, is still in wide use today. However, several newer designs are now available that optimize for convenience and for diffusion- or electrophysiology-based measurements....

Ussing Chambers Systems from Warner (continued) The Components The Chamber System While decisions need to be made for each step in assembling a complete Ussing system, the greatest complexity is associated with choosing the chamber and its associated tissue support. Once these elements have been addressed, then the task of selecting the other components (amplification, data acquisition, etc) is more or less direct. All Ussing chamber systems have the following features in common: a means to hold the membrane while minimizing tissue damage, a means for holding and exchanging solutions, a...

Classic Ussing Systems The investigation of ionic transport across epithelial cells was greatly facilitated by the development of a chamber system designed by Dr. H. H. Ussing more than 40 years ago. Today this equipment bears his name. The U9500 ʻClassicʼ design presented here varies little from Dr. Ussingʼs original equipment which remains in wide use for the study of electrophysiological properties of epithelial tissues. A newer design, the U9520 ʻClassic with Insertsʼ allows for insertion of cell culture or tissue supports and greatly increases the functionality of the classic Ussing...

Classic Ussing Systems U9500 & U9520 (continued) • Select Base Assembly with desired reservoir size. Systems include support stand with rods, circulation reservoir, 3-prong clamp, U9303 Air/Gas Fitting Kit, U9975A Electrode Set, and U9965EP Electrode Bridge Fitting Kit • Select chamber. Choices are ‘Classic’ or ‘Classic with Insert’. Both available with or without drains • Finally, if using Classic with Insert’, select desired insert Base Assembly Order # 64-0500-R4 64-0500-R10 64-0500-R20 Description Classic Ussing base assembly with 4 ml circulation reservoir Classic Ussing base assembly...

Classic Ussing Systems Replacement Parts Electrode bridge fittings, pkg. of 12, and adapters, pkg. of 6 64-0554 U9975A Electrode set, 2 Ag/AgCI pellet, 2 Ag wire 64-0555 U9985 Bridge fitting adapters, pkg. of 6 Condenser set for 4 ml circulation reservoir, set of 2 64-0560-10ml U9302-10 Condenser set for 10 ml circulation reservoir, set of 2 64-0560-20ml U9302-20 Condenser set for 20 ml circulation reservoir, set of 2 64-0562 U9403 Support stand with one stand rod Warner Instruments • Phone (203) 776-0664 • Toll Free U.S. (800) 599-4203 • Fax (203) 776-1 278 • www.warneronline.com

Self-Contained Ussing Chambers U9926 & U2500 U9926 Single Channel Chamber U2500 Dual Channel Chamber (2 inserts) The Self-Contained Ussing Chamber Systems U2500 Dual Channel Chamber (2 Inserts) Exploded View The Self-Contained Ussing Chambers systems come in single- and dual-channel models and offer many advantages over the Classic design. The single-channel U9926 and dual-channel U2500 are shown above. Both systems make use of inserts to secure and position the tissue or culture cup Chamber Half-Cell Reservoir Cover Ag Wire (Current Electrode) Electrode Bridge Fittings Water Jacket Drain...

Self-Contained Ussing Chambers Standard Accessories Supplied with U9926 and U2500 The U9926 (single-channel chamber is supplied with 1 electrode set (consisting of 2 voltage and 2 current electrodes), 12 bridge fittings with adapters; and a tubing/ fitting kit. The U2500 (dual channel) chamber is supplied with two each of the above described accessories. Chamber inserts are ordered separately. • Select Base Assembly. Each system includes one or two U9975A Electrode Set(s) and U9965EP Electrode Bridge Fitting Kit(s) • Select Insert. Order 1 if using the U9926 and order 2 if using the U2500...

Multichannel Ussing Chambers Warner Instruments offers three multichannel Ussing chamber systems capable of supporting up to 24 independent chambers, all operating in parallel.Two systems position the membrane vertically and one positions the membrane horizontally. All three multichannel chamber systems embody the self-contained design and are fully capable of supporting either membranes or cell culture cups.The differences between these systems are explained below. NaviCyte Horizontal and Vertical Chamber Systems The Harvard/NaviCyte patented line of horizontal and vertical diffusion...

Multichannel Ussing Chamber This system features the ability to run up to six chambers simultaneously and is optimized for transport studies on cells and tissues exposed to an air interface. The included heater block permits temperature control, with the use of a circulating water bath, for these chambers in both open and closed configurations. While optimized for diffusion-based studies, the use of optional electrodes permits electrophysiological membrane resistance measurements. The NaviCyte Horizontal chamber system consists of a heater/support block and up to six horizontal chambers....