Warner Instruments Live Cell Imaging Guide
16Pages

Integrated Components for Live-Cell Imaging chambers, perfusion and temperature control

Recording / Imaging Chambers Modular Design Warner’s open bath recording chambers are a family of chambers designed to fill a large number of imaging and recording needs. The modular design consists of two parts; a polycarbonate chamber (insert) and an anodized aluminum platform (holder). The platform clamps the chamber providing a seal between the chamber and coverslip. Heated platforms are used where chamber warming is required. The design of the platforms permits quick removal of the chamber for exchange of the coverslip. Stage Adapters are designed to allow the mounting of Warner...

An Integrated Systems Approach Platforms function as the base for Series 20 Chambers and provide clamping to make a seal between the chamber and coverslip. Stage Adapters Stage adapters are available for all major microscopes and stages currently on the market. Integrated Components for Live Cell Imaging Heated Platform Cable Assembly Recording Chamber and Slice Anchor The CC-28 cable provides power to the heated platform. There are also 2 thermistors on the cable used to maintain and monitor the desired temperature of the sample. Our chamber selection guide, available online and in our...

Open Bath Recording / Imaging Chambers Designed For: • Electrophysiological recordings • Optical imaging • Tissue slice and cell cultures • Low profile permits maximum electrode access to the bath • Stage adapters for all microscope stages Suction Tube Open Bath Recording and Imaging Chambers Warner open bath chambers incorporate diamond-shaped fluidics yielding laminar flow throughout the bath. The low profile design allows for excellent electrode access without interfering with optical measurements. Chambers with larger baths (e.g. RC-26) allow for insertion of round coverslips into the...

Closed Bath Imaging Chambers Suction Tube Perfusion Input Image courtesy of John Geible using the JG-23 chamber. Suction Reservoir The RC-20 and RC-20H are very low volume imaging chambers featuring fast solution exchange and short working distances. An access port (Hamilton syringe compatible) at the bath input may be used to inject substances into the solution flow or to remove gas bubbles. Integrated Components for Live Cell Imaging • Closed bath chamber designs • Diamond fluidics • Ideal for fluorescence, calcium, and time-lapse imaging studies • Stage adapters for all microscope stages...

Field Stimulation Imaging Chambers • Designed for studies involving cardiac myocytes • Open and closed bath chamber designs • Ideal for fluorescence, calcium, and time-lapse imaging studies • Stage adapters for all microscope stages Suction Tube Perfusion Input Secondary Perfusion Input The RC-21BRFS is a small volume, closed bath imaging chamber designed for field stimulation studies. It uses a pair of 25 mm round coverslips to form the top and bottom of the chamber, and the total bath volume is 263 µL. Two platinum wire electrodes are attached to the parallel sides of the oval-shaped...

Valve Controllers VC-8 Valve Control System The VC-8 Valve Control System lies at the heart of a multi-valve perfusion system designed to automate and control the delivery of solutions to imaging and recording chambers. Its flexible design allows the system to be used in diverse applications. Integrated Components for Live Cell Imaging Perfusion Control Systems • Integrated spill sensor • Choice of 3 valve types • 8 channel system • Manual and computer controllable The complete system includes a powered valve controller, a valve bracket with valves, connecting cable, C-Flex“ tubing, MP...

Closed Bath Chambers for Confocal Imaging Headline Designed for • Shear/flow studies • Optical imaging • Cell culture studies • Fits all Nikon microscopes The RC-30HV has a 17.7 mm Viewing Aperture and uses 22 x 30 mm and 22 x 40 mm coverslips. RC-30HV The RC-31 has a 25 mm Viewing Aperture and uses 30 mm top coverslip and 40 mm bottom coverslip. Image courtesy of Alison North Rockefeller University. The RC-30, and RC-31 closed bath chambers are low profile with special features for use with confocal microscopes. The closed bath design allows for the use of high NA apertures with a minimum...

ProFlow Shear Flow Chamber Integrated Components for Live Cell Imaging • Computer designed gaskets optimized for well-defined, well-controlled shear-flow • Based on technology developed at the Case Western Reserve University • Single- and dual-channel capable chamber • Made from polycarbonate for easy cleanup Warner’s new PFC-1 ProFlow chamber is based on the designs of Dr. Melissa Knothe Tate, currently of the Case Western Reserve University. The PFC-1 Proflow Chamber uses silicone gaskets, similar in approach to our popular RC-30 chamber, to form a closed-bath area sandwiched between two...

Quick Exchange Heated Platform with Perfusion > Model QE-1 Designed For: • Cell culture studies • Rapid screening assays • Quick replacement of dish or chamber • Series 40 chambers and 35 mm culture dishes • Open design for easy access Quick Exchange Platform The QE-1 is a versatile base for both RC-40 chambers and 35 mm glass bottom culture dishes. The platform is at home on both upright and inverted microscopes and includes resistive heating elements. The base has a 25 mm aperture, tapered on the bottom to allow objective access and a magnetic stainless steel strip along its upper...

Heated/Cooled Platforms Designed For: • Cell / tissue culture studies • Rapid screening assays • Temperature control from 5° to 50°C • 35 mm culture dishes / chambered slide designs • Open design for easy access Heated and Cooled Platforms Warner’s peltier-driven platforms offer the ability to cool specimens which may be sensitive at ambient temperature. Peltier-cooled stages include an integral water jacket that when coupled to a secondary liquid cooling module, LCS-1 offer rapid cooling of specimens. Precise temperature control is possible when used with our low noise CL-100 single...