Isolated Lung Perfusion Systems for small to large animal models Hypoxic Vasoconstriction Resistance & Compliance Pulmonary Metastases Respiratory Mechanics Vascular Permeability Surfactant Bioactivity Inhalation Toxicology Inhalation Therapy Lung Regeneration Tissue Engineering Lung Preservation Edema Formation Drug Absorption Wg^\ HUGO SACHS ELEKTRONIK HARVARD V ~7 The Physiology Specialists APPARATUS

ISOLATED LUNG PERFUSION SYSTEM INTRODUCTION TO THE ISOLATED PERFUSED LUNG (IPL) The IPL method has been found to be invaluable in characterizing the non-respiratory capabilities of pulmonary tissues such as pulmonary metabolic activity as well as the activities of various components (pulmonary alveolar macrophage, alveolar tissue, endothelial tissue, etc) in response to inhaled/airborne particulates/therapies. Isolated lung systems are equally useful for evaluating respiratory functions such as respiratory mechanics and gas exchange. The lungs, while being removed from the body, are still an...

The IPL preparation requires both the perfusion of the vascular system as well as the ventilation of the respiratory tract. Like all HSE perfusion systems the IPL series utilizes a Solid State Physiological Perfusion Circuit (S2P2C) technology, defined by the use of a solid block of material (Perspex) with precision-milled perfusion and ventilation pathways. The S2P2C is integrated into a common architecture, appropriately scaled for species, ensuring precisely repeatable non-turbulent perfusion and ventilation for the highest fidelity pressure and flow measurements. This, combined with the natural...

INTRODUCTION TO IPL ISOLATED LUNG PERFUSION SYSTEM Perfusion System Ventilation System Data Acquisition System Nutrition Solution Data Acquisition System Perfusion Flow Flow Meter Flow Probe Perfusion Pressure Bridge Amplifier Lung Weight Bridge Amplifier Bridge Amplifier Pleural Pressure Bridge Amplifier Differential Pressure Transducer Differential Pressure Transducer Pressure Transducer Weight Transducer ISOLATED LUNG PERFUSION SYSTEM Block Diagram for the Isolated Perfused Lung Micro Electrodes Negative Pressure Ventilator Pleural Pressure Online Calculated Parameters: • Tidal Volume • Compliance...

IPL-1 Isolated Lung Perfusion System PRESSURE BALANCED • Pressure-balancing vessel eliminates transmural pressure difference during subatmospheric ventilation • Allows for simulation of hypertensive cardiac afterload POSITIVE & NEGA TIVE PRESSURE VENTILA TION • Option for positive and negative pressure ventilation with one-step switch OPTIONS • Simple add-on Aerosol Nebulizer • Multi-Gas for hyperoxic, hypercapnic, anesthetic or other alternative gas studies ISOLATED LUNG PERFUSION SYSTEM ISOLATED LUNG PERFUSION SYSTEM UNIQUE CANNULAE • Cannulae are matched to the size of mouse vessels • Tip...

ISOLATED LUNG PERFUSION SYSTEM IPL-1 BASIC: The Most Popular Mouse Isolated Perfused Lung System MEASURED SIGNALS & PARAMETERS ON AN IPL-1 BASIC SYSTEM: The Following Signals are Recorded as Raw Data: • Pulmonary Artery (Perfusion) Pressure • Perfusion Flow • Intrapleural (artificial thorax) Pressure or Tracheal Pressure • Respiratory Flow The Following Parameters can be calculated from the Raw Data*: • Tidal Volume, Minute Volume • Respiratory Volume • Peak Inspiratory & Expiratory Airflow • Vascular Resistance • Respiration Rate The IPL-1 is engineered for the most demanding applications. As...

Basic IPL-1 System Includes: Negative Pressure Ventilation Controller with Pump to Core IPL-1 & 2 • IPL-1 Base Unit for Isolated Mouse Lungs Choose this ventilation option when: * performing standard positive- and negativepressure ventilation studies • Analog Roller Pump • Perfusion Pressure Measurements - Low Range Pressure Transducer - TAM-D PLUGSYS Transducer Amplifier Module • VCM-P PLUGSYS Ventilation Control Module with Integral Pump • Perfusion Controller Module, SCP (for constant flow or constant pressure perfusion) • TCM PLUGSYS Timer Counter Module for introduction of periodic Sigh...

ISOLATED LUNG PERFUSION SYSTEM Option AG-1: 73-4294 Aerosol Nebulizer Kit to IPL-1 FEATURES & BENEFITS • Low particle sizes (100% of the particles are below 10 µm) No Solution Warming Required Recommended for Nebulizing Drugs Sensitive to Ultrasonics Aerosol is Automatically Transported by Compressed Air at a Pressure of 1.5 bar (22 PSI) • Multi-Gas Adapter and Connection Kit for IPL-1 • Positive Pressure Ventilation to IPL-1 • Choose this ventilation option when only positive pressure ventilation is desired Includes: • Minivent Mouse Ventilator • Y-Adapter to Connect External Ventilator FEATURES...

Option pHC-1: 73-4308 pH Control Unit to Core IPL-1 System FEATURES & BENEFITS • CO2 Delivery to Maintain pH when System is not Deoxygenated with N2/CO2 Gas Mixture pHCM Controller Delivers Gas (Commonly CO2) by Bubbling into Mixed Buffer to Maintain a Desired pH Set Point Dictated by pHCM Module Minimizes Foaming of Perfusates due to Minimal Gassing of 100% Gas to maintain pH ISOLATED LUNG PERFUSION SYSTEM Includes: • pHMM PLUGSYS pH Measurement Module • pHCM PLUGSYS pH Control Module • Flushtrode® pH Electrode & Connection Cable • Electrolyte • Modified Cover to 0.5 L Reservoir • Utilizes 4...

IPL-2 Isolated Lung Perfusion System NEGATIVE PRESSURE VENTILATION • Option for positive and negative pressure ventilation with one-step switch • Configurable periodic sigh (hyperinflation) breaths to minimize edema formation VENTILATION MODE • Simple Switch Allows Instant Changeover from Positive Pressure to Negative Pressure Ventilation CONSTANT FLOW OR PRESSURE PERFUSION UNIQUE LUNG CHAMBER • Quick switch from constant flow to constant pressure for maximum application flexibility • Fully-Thermostated Lung Chamber • Unique Chamber Lid Incorporates: - Heating Coil/Bubble Trap - Pneumotachometer...