- Catalogs
- Harvard Bioscience, Inc.
- Hugo Sachs Elektronik/Harvard Apparatus Isolated Heart Perfusion Systems Guide
Hugo Sachs Elektronik/Harvard Apparatus Isolated Heart Perfusion Systems Guide
Hugo Sachs Elektronik/Harvard Apparatus Isolated Heart Perfusion Systems Guide
The document provides a comprehensive overview of the Isolated Heart Perfusion Systems developed by Hugo Sachs Elektronik, a Harvard Apparatus company. These systems are designed for cardiac physiology research across various animal models, including mice and rabbits. Key applications include cardioplegia, tissue engineering, contractility studies, and cardiovascular screening.
The IH-Series features Perspex construction for a Solid State Physiological Perfusion Circuit (S2P2C), ensuring a non-turbulent perfusion pathway and accurate pressure and flow measurements. The system's thermal properties enhance physiological relevance.
The IH-Series includes Langendorff and Working Heart configurations. The Langendorff system is used for retrograde perfusion, while the Working Heart system allows for orthograde perfusion, enabling physiological assessments of ventricular contractility. A Biventricular Working Heart system is introduced for studying diseases involving pulmonary vascular dysfunction.
The systems offer extensive measurement capabilities, including Left Ventricular Pressure (LVP), Pressure Volume (PV) parameters, ECG analysis, and Monophasic Action Potential (MAP) analysis. Online calculated parameters include heart rate, contractility index, coronary resistance, and mean pressures/flows.
Various system configurations are outlined, such as the UP-100IH Basic Langendorff for small animal models and the IH-SR and IH-5 systems for advanced applications. Each system can be customized with additional options to suit specific research needs.
The IH-Series offers a versatile and advanced platform for cardiac research, providing accurate and physiologically relevant data for a wide range of research applications.
The UP-100IH system includes a base unit, heart chamber, thermocirculator, analog roller pump, compliance chamber, and pressure measurement tools. The IH-SR system features a double-walled glass reservoir, roller pump with feedback control, and a modular design for future upgrades.
The systems support various experimental setups, including Langendorff assays and cardiomyocyte isolation. The UP-100IH can be equipped with a constant pressure/flow controller for precise perfusion management. The IH-SR system can be expanded to a full ejecting working heart system.
Both systems offer options such as direct coronary flow measurement, oxygenating systems for foaming perfusates, and cell isolation kits. Additional features include drug addition pathways, pacing, and metabolic monitoring capabilities.
Researchers planning to upgrade to full working heart functionality are advised to start with a working heart-ready Langendorff system to avoid duplication of functionality and reduce costs. Technical assistance is available for system configuration and custom design requests.
The document cites several studies utilizing these systems, highlighting their application in various research contexts.
The IH-5 system is designed for rabbit, rat, or guinea pig hearts, allowing for constant pressure and flow perfusion. It is suitable for studies involving hypertensive animals with perfusion pressures up to 300 mmHg.
Features include an integrated small volume aortic block, optimized cannulae design, proprietary mini holders, and a drug injection pathway. The system is upgradeable to a Working Heart System for enhanced experimental sensitivity.
Records signals such as Isovolumetric Left Ventricular Pressure, Aortic Pressure, and Coronary Flow. Calculates parameters like dLVP/dt, Heart Rate, Mean Perfusion Pressure, and Coronary Resistance.
Langendorff and Working Heart-Ready configurations are available, with species-specific additions for rat/guinea pig and rabbit hearts. Direct and indirect flow measurement options are included.
The IH-5 system is ideal for cardiac electrophysiology and safety pharmacology research, providing stable perfusion conditions and the ability to conduct long-term recordings with minimal artifacts.
The EasyCell System is designed for isolating cardiomyocytes from small rodents. It is compact, easy to set up, and maintain, conserving bench space. The system includes a perfusion circuit for clearing the heart of blood and tissue disintegration.
Components include the EasyCell System Base, Glassware, Jacketed Protease Reservoir, Buffer Reservoir, Aortic Cannula Set, Thermocirculator, Digital Peristaltic Pump, and Mini Magnetic Stirrer.
Kits are available to customize or expand the system's functionality, including barbed connector kits and luer stopcock kits for compound addition or adaptation for multiple species.
These kits include connectors made from materials like polypropylene, nylon, and Kynar®, available in various sizes to fit different tubing diameters.
The systems support research fields such as cardiovascular screening, tissue engineering, and electrophysiology, aiding studies on ischemia, reperfusion, and transgenic phenotyping.
Catalog excerpts
Isolated Heart Perfusion Systems for mouse to rabbit animal models Refractory Period Contractility Studies ^^HP* Safety Pharmacology Myocyte Harvesting & Isolation W§i£\ HUGO SACHS ELEKTRONIK H A RVA RD ~7 The Physiology Specialists APPARATUS
Open the catalog to page 1
ISOLATED HEART PERFUSION SYSTEM ISOLATED HEART PERFUSION SYSTEMS BY HUGO SACHS ELEKTRONIK – HARVARD APPARATUS Hugo Sachs Elektronik, a Harvard Apparatus company, has always provided top-notch, fully integrated physiology research systems. Continuing our tradition of innovation and high performance we are happy to unveil the newly configured line of Isolated Perfused Heart Systems for rodent and rabbit models. For two decades the Hugo Sachs Elektronik IH-Series of Isolated Heart Systems has been the gold standard for cardiac physiology measurements in both Langendorff and Working Heart perfusion...
Open the catalog to page 2
The IH-Series Ex Vivo Perfused Heart Systems always start as a Langendorff system. This means a configuration solely for retrograde perfusion down the aorta (toward the heart). In this mode, the aortic valve prevents the perfusate (nutrient solution) from entering the left ventricle, but the coronary arteries are perfused via the coronary ostia which are located just outside the valve, thus maintaining the viability of the heart muscle. Perfusate drains as effluate from the coronary circulation via the coronary sinus and pulmonary artery tends to drip from the apex of the heart making it easily...
Open the catalog to page 3
ISOLATED HEART PERFUSION SYSTEM Langendorff System Data Acquisition System Isovolumetric Left Ventricular Pressure (LVP) Bridge Amplifier Pressure Transducer Nutrient Solution Perfusion Flow Flow Probe Perfusion Pressure Bridge Amplifier Pressure Transducer Electrogram Lead II (ECG, up to 12 Lead) Dissolved Carbon Dioxide (pCO2) Biopotential Amplifier Monophasic Action Potential (MAP, up to 8) Online Calculated Parameters: • dP/dt Max, Min • Systolic, Diastolic LVP • ECG Analysis • MAP Analysis • Heart Rate • Contractility Index, Tau • End Systolic & Diastolic Flows • Coronary Resistance • Mean,...
Open the catalog to page 4
Pressure Transducer Bridge Amplifier Flow Probe Data Acquisition System Preload Pressure Atrial Flow Bridge Amplifier Flow Probe MPVS or Bridge Amplifier Left Ventricular Pressure (LVP) or Pressure Volume (LVP-PV) Electrogram Lead II (up to 12 Lead) Pressure Transducer ISOLATED HEART PERFUSION SYSTEM Working Heart Nutrient Solution Aortic Pressure Aortic Flow Monophasic Action Potential (MAP, up to 8) Biopotential Amplifier Dissolved Carbon Dioxide (pCO2) Online Calculated Parameters: • dP/dt Max, Min • Systolic, End Diastolic LVP • PV Parameters • ECG Analysis • MAP Analysis • Contractility...
Open the catalog to page 5
ISOLATED HEART PERFUSION SYSTEM Langendorff Systems UP-100IH BASIC LANGENDORFF The UP-100IH Basic Langendorff perfusion system is primarily for rat, guinea pig, and juvenile rabbit heart, though it can be used for mouse heart as well. It has a small footprint, making it ideal for multi-channel compound screening that can incorporate measurement of basic cardiac parameters such as LVP and ECG. SEE PAGES 8-13 IH-SR LANGENDORFF The IH-SR Isolated Heart for Small Rodents, Langendorff Only, is designed to be used for rodent models mouse, rat, and guinea pig exclusively in the Langendorff retrograde...
Open the catalog to page 6
IH-SR WORKING HEART SYSTEM The Working Heart model is the ultimate ex vivo physiological model. The IH-SR Working Heart allows the researcher to take full advantage of this model with physiological simulation that cannot be matched in small rodent preparations. With the built-in patented Physiological Afterload System (Starling resistor), the need for the conventional water column is eliminated. The result is vastly improved arterial pressure evaluation without the risk of damage to heart valves. SEE PAGES 36-41 ISOLATED HEART PERFUSION SYSTEM Working Heart Systems IH-5 WORKING HEART SYSTEM The...
Open the catalog to page 7
UP-100IH Universal Perfusion System PERFUSION PATH • Compliance Chamber for Pressure Pulse Dampening of Peristaltic Pump • Primary and Secondary Bubble Traps Protect Against Embolism, Ensure Vascular Integrity • System Volume is <20 ml, Reducing Temperature Loss and Mixing of Test Compounds HEART CHAMBER • Small Heart Chamber For Temperature Maintenance • Precision Ball-Joint System for Secure Positioning of Electrodes • Perfusion Pressure Measurement Proximal to Aorta ISOLATED HEART PERFUSION SYSTEM ISOLATED HEART PERFUSION SYSTEM FEATURES: • Most Compact Langendorff Design • Versatile Perfusion...
Open the catalog to page 8
ISOLATED HEART PERFUSION SYSTEM UP-100IH: Most Popular System for Multi-Channel Compound Screening The UP-100IH Advanced system allows up to the full range of available measured parameters beyond the basic system including direct coronary flow, ECG lead II, temperature measurement, and metabolic parameters (pH, pO2, pCO2). All UP-100IH systems can be used for constant pressure or constant flow perfusion of isolated hearts of mice, guinea pigs, rats and newborn rabbits as long as the coronary flow is below 50 ml/min. Constant pressure perfusion is achieved by the optional addition of a controller...
Open the catalog to page 9
UP-100IH Core System, Basic: 73-4384 (115V) or 73-4385 (230V) UP-100IH Core System, Advanced: 73-4386 (115V) or 73-4387 (230V) Includes: • UP-100 Base Unit • Heart Chamber and Holder to UP-100 System PLUGSYS Modular Amplifier System • UP-100 Addition for Langendorff (with cannulae for rat/guinea pig) • Thermocirculator • Analog Roller Pump ISOLATED HEART PERFUSION SYSTEM • Compliance Chamber (Windkessel) • Perfusion Pressure Measurements ° Pressure Transducer ° TAM-D PLUGSYS Transducer Amplifier Module • PLUGSYS Basic System Case (Core System 6 Slot Units) - UP-100IH Basic: PLUGSYS 601 Case,...
Open the catalog to page 10
ISOLATED HEART PERFUSION SYSTEM UP-100IH: Options SCP 73-2806 Constant Pressure/Flow Controller Choose this option if you require the ability to perfuse a heart in constant pressure mode: • Allows operation in constant pressure or constant flow with simple switch • Additional measured signal/calculated parameters ° Indirect Coronary Flow measurement ° Calculation of Coronary Resistance • Accurate control of perfusion pressure or flow, even at very low flow rates • Flexible perfusion circuit setup adjusts to suit individual perfusion conditions Fiber Oxygenator mounted to top plate of IH-SR Includes:...
Open the catalog to page 11All Harvard Bioscience, Inc. catalogs and technical brochures
Biowave 3 & Biowave 3+2 Pages
Scie-Plas Product Catalogue132 Pages
CMA Microdialysis Catalog56 Pages
EZ READ 400 ELISA3 Pages
- Automation software solution
- Analysis software solution
- Liquids analyzer
- Rack system
- Windows software
- Automatic analyser
- Control software
- Solids analyser
- Measurement software
- Laboratory analyzer
- Development software
- Spectrophotometer
- Analyzer for the food industry
- Data acquisition software
- Visible spectrophotometer
- UV spectrophotometer
- Modular rack system
- Hydrometer
- Mobile rack system
- Laboratory software