Hugo Sachs Elektronik/Harvard Apparatus Cardiomyocyte Isolation Systems Brochure
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Isolation Systems for mouse to small rabbit heart HUGO SACHS ELEKTRONIK HARVARD \~ ~/ The Physiology Specialists APPARATUS

CARDIOMYOCYTE ISOLATION SYSTEMS PSCI PERFUSION SYSTEM FOR CARDIOMYOCYTE ISOLATION The PSCI is designed for pharmacology labs that require reproducible, high-yield viability for their cardiomyocyte assays. The PSCI has two separate perfusion pathways in order to separate clearing buffer from collagenase such that the solutions mix in less than 500 µl, with a simple switch between the two. All solutions recirculate to the aerated reservoir so that they are fresh at all times. The wetted path is compatible with ethanol allowing for a sterile preparation. The system perfusate volume is < 3 ml...

PSCI: Perfusion System for Cell Isolation DUAL HEAT  EXCHANGER • Thin-Walled Stainless Steel Heat Exchanger Provides Quick Heating with Minimal Volume • Interchangeable Insert Allows Switchover Between Mouse and Rat Heat Exchangers to Optimize Priming Volumes for the Two Species CARDIOMYOCYTE ISOLATION SYSTEMS CARDIOMYOCYTE ISOLATION SYSTEMS PSCI PERFUSION PERFUSION HUB COLLAGENASE RESERVOIR • olid State Perfusion Circuit Ensures S Thermal Stability, Laminar Flow, and Minimal System Volume • ubble Traps for Each Perfusate B Stream Protect Heart from the Possibility of Air Emboli, Maximizing...

CARDIOMYOCYTE ISOLATION SYSTEMS PSCI: Perfusion System collected in the collagenase reservoir to which the dissected left ventricle is added for further dissociation, allowing an increased total cell yield. The apparatus is so designed that the individual steps required for preparing the cells can proceed as simply and clearly as possible. With two separate perfusion circuits, the organ can be switched between clearing buffer for removal of blood cells and collagenase buffer for organ digestion by means of a custom, precision-milled changeover stopcock. Using the standard configuration,...

PSCI PERFUSION Core system PSCI for mouse cardiomyocyte isolation Choose this option if you require the ability to perfuse a heart in constant pressure mode. This option requires option PSCI-PP (IH2 73-4428) pressure measurement Includes: • PSCI-MH Base System for mouse heart Addition for constant pressure perfusion to PSCI • llows operation in constant pressure or constant flow A with simple switch • Analog Roller Pump and Tubing (Tygon) • dditional Measured Signals A • Aortic Cannula for mouse heart, 1.0 mm • Protease Reservoir • Mini Magnetic Stirrer • Holder for Pressure Transducer •...

CARDIOMYOCYTE ISOLATION SYSTEMS EasyCell: Simple System for Cell Extraction PULSATION DAMPENING • ir Vessel/Compliance Chamber A Dampens Perfusion Pressure Pulsation Generated by the Peristaltic Pump • erves as a Primary Bubble Trap S to Prevent Air Emboli from Causing Regional Ischemia, Increasing Potential Cell Yield TEMPERATURE CONTROL • ater-Jacketed Heat Exchanger W and Heart Chamber for Maintenance of Physiological Temperature FEATURES & BENEFITS • esigned for Cardiomyocyte D Isolation from Small Rodent Hearts • an be Adapted for Other Small C Rodent Organs In Situ or Ex Vivo • imple...

a warmed, aerated standard perfusion buffer for blanching/clearing the heart of blood and then with a warmed, aerated protease solution for tissue disintegration. Unlike the PSCI system, the clearing buffer and collagenase share the same perfusion line, slightly delaying the delivery of the collagenase after switching over from the clearing buffer. The wetted components of the perfusion circuit can be flushed with a 70% ethanol solution or autoclaved for sterilization purposes should the cells need to be incubated for a number of hours following isolation rather than for immediate use. The...

HUGO SACHS ELEKTRONIK HARVARD The Physiology Specialists APPARATUS 84 October Hill Road email: physiology@harvardapparatus.com Isolation Systems for mouse to small rabbit heart www.hugo-sachs.de • www.harvardapparatus.com © Copyright Hugo Sachs Elektronik - Harvard Apparatus