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Harvard Apparatus Sample Preparation Catalog
Harvard Apparatus Sample Preparation Catalog
The document provides an overview of sample preparation products by Harvard Apparatus, emphasizing the removal of impurities from biological samples for accurate analysis.
Harvard Apparatus offers solutions like equilibrium dialysis products for ligand binding studies, trace enrichment, de-salting, buffer exchange, and removal of unwanted molecules.
- Pipette Tips: Includes PrepTips™, ProTips™, and TipColumns™ for chromatographic separation.
- SpinColumns™: Available in various formats for high-throughput sample preparation.
- Filtration Products: Solutions for different sample volumes and types.
- Dialysis Products: Reusable and disposable options, including Ultra-Fast and Spin DIALYZERS™.
- Liposomes and CoZap™: Products for liposome preparation and contaminant removal.
Used for educational and research purposes, these models provide accurate molecular structure representations.
Guides for selecting sample preparation technologies based on speed, sample volume, and solution compatibility.
Harvard Apparatus offers a wide range of products for effective sample preparation, catering to various research and industrial needs.
- Single Use and Reusable Chambers: Various chambers for specific sample volumes with different materials.
- Membrane Specifications: Details on molecular weight cut-off for membranes.
- Ordering Information: Includes components like chambers, membranes, and additional equipment.
- Reusable and Single-use Products: Products for protein binding studies with details on sample volumes and membrane requirements.
- Materials and Membranes: Use of materials like PTFE and Delrin™ with various membranes.
- PrepTip™ and ProTip™: Designed for rapid sample preparation, including protein purification and mass spectrometry.
- Ultra-Micro & Micro TipColumns™: Suitable for small sample volumes with various purification needs.
- Ease of use with no special equipment required beyond standard laboratory tools.
- Wide range of applications including protein purification and mass spectrometry.
- Available in various sizes and configurations.
SpinColumns are designed for purifying small samples and are available in multiple formats, compatible with standard centrifuges.
- Ultra-Micro SpinColumns: For sample volumes from 10 µl to 25 µl.
- Micro SpinColumns: For sample volumes from 25 µl to 75 µl.
- Macro SpinColumns: For sample volumes from 75 µl to 150 µl.
- 96-Well Micro SpinColumns: For high throughput applications.
- 384-Well SpinColumns: For high throughput applications.
- Easy to use with multiple formats available.
- High sample recovery and rapid preparation time.
- Applications include DNA, protein, and peptide purification.
- Apply the sample to the SpinColumn.
- Centrifuge briefly to separate the sample.
- The column material binds and purifies the sample.
- Specifications: Filter plates in 96-well or 384-well formats with various membrane pore sizes.
- Applications: Used for nucleic acid binding, DNA/RNA purification, and more.
- Procedure: Involves filling filter plate wells and applying vacuum.
- Accessories: Includes reservoirs, storage plates, and lids.
- Introduction: Used for separating small molecules from larger ones.
- Reusable Dialyzers: Includes DIALYZER, Ultra-Fast DIALYZER, and SpinDIALYZER.
- Disposable Dialyzers: Options for single-use applications.
- Applications: Used for buffer exchange, sample concentration, and purification.
Devices with magnetic features for faster dialysis times, offering ease of use and high sample recovery.
Designed for rapid dialysis with a large surface area, suitable for buffer exchange and salt removal.
Single-use devices for small sample volumes, offering high sample recovery.
Designed for larger samples with features similar to smaller units.
Allows simultaneous preparation of 96 samples, suitable for high-throughput applications.
Details on various membrane types and their applications.
Includes the Membrane Minder and Concentrator for sample concentration.
Details on liposome preparation using various lipid mixtures and detergents.
Liposome size can be adjusted by varying parameters like dialysis rate and detergent type.
Gentle detergents are used to avoid hydrolysis or peroxidation.
Includes Mini Lipoprep and Liposomat for different volume requirements.
Used for rapid removal of Coomassie Blue stain from electrophoresis gels.
Versatile technology for rapid purification of biomolecules.
Different configurations for the ElectroPrep system tailored for specific purification needs.
- Connectors and Membranes: Includes connectors for ElectroPrep DIALYZERS and various membranes.
- Membrane Types: Details on regenerated cellulose, cellulose acetate, and polycarbonate membranes.
- Fast Flow-Thru DIALYZER: Designed for electro-elution and other processes.
- Equilibrium Dialysis: Used for studying binding interactions.
- Use regenerated cellulose membranes for organic solvents.
- Cellulose acetate membranes for aqueous solutions.
- Polycarbonate membranes for defined pore sizes.
Detailed ordering codes for various dialyzers and membranes.
Suitable for protein-drug binding assays, receptor binding assays, and more.
Includes Teflon Macro Dialysis Cells and other components for interaction studies.
Single-use product for interaction studies with high sample recovery.
Allows simultaneous assay of 96 samples, preventing cross-contamination.
Details on atomic models for research and teaching purposes.
Order numbers and pricing for various products, including contact information for Harvard Apparatus.
Information on assembled nucleic acid helices and components.
Details on individual L-amino acids and their assembled forms.
Outlines unassembled nucleic acid helices and components.
Specifications for carbon, hydrogen, halogen, and nitrogen atoms.
Availability of custom assembled models for various molecules.
Contact details for Harvard Apparatus for further inquiries or orders.
- Hydrogen H-Bond: Used with various nitrogen types for conversions.
- Amine Cap: Used with nitrogen types for conversions.
- Oxygen Atoms: Different types of oxygen atoms described.
- Metal Atoms: Specifications for all-purpose, ionic, and covalent metal atoms.
- Phosphorus and Sulfur Atoms: Details on arrangements and bond angles.
- Various types of connector links described for molecular model construction.
- Includes tools like the Construction Tool and Hydrogen Wrench.
- Research and teaching sets available for educational purposes.
- Tables provide specifications for covalent radii and bond angles.
- Order numbers and product descriptions for easy reference.
Catalog excerpts
Sample Preparation Products Packed Pipette Tips SpinColumns™ Filtration Products DIALYZERS™ Liposomes CoZap™ ElectroPrep™ System Equilibrium DIALYZERS™ CPK® Atomic Models Call to receive other catalogs of interest: Cell Biology & Electrophysiology Physiology & Pharmacology Behavioral Research Micro/Nano Fluidics Electroporation & Electrofusion Molecular Sample Preparation
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table of contents Sample Preparation Table of Contents Item Introduction Guides: Applications, Technology & Products Packed Pipette Tips PrepTips™ ProTips™ TipColumns™ SpinColumns™ Single SpinColumns 96-Well and 384-Well SpinColumns Filtration Products Re-Usable DAILYZERS™ DIALYZERS Ultra-Fast DIALYZERS Spin DIALYZERS Flow-Thru DIALYZERS Single Use DIALYZERS™ Ultra-Micro DispoDIALYZERS Micro DispoDIALYZERS Fast Macro DispoDIALYZERS 96-Well DispoDIALYZERS Liposomes CoZap™ 41 ElectroPrep™ System Re-Usable Equilibrium DIALYZERS™ Fast Micro-Equilibrium DIALYZERS Multi-Equilibrium DIALYZERS Single...
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The Sample Preparation Target materials in biological samples are commonly present in trace amounts among larger quantities of numerous impurities or components such as: • Polyacrylamide Gels Excess Dyes Small undesired molecules like primers, peptides, inhibitors & drugs There is currently no single method of purification that is applicable to all samples. Appropriate sample preparation methods are used depending on the type of target material and the sample composition. These impurities or components may have to be completely removed before analysis. Some target materials must also be enriched...
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sample preparation Application Guide for Sample Preparation of Biological Mixtures i.e. Proteins, Nucleic Acids, Carbohydrates Acrylamide Removal Affinity Purification Antibody Production Buffer Exchange Cancer Therapy Carbohydrate Purification Cell-Cell Interactions Cosmetics CsCI Removal Destaining Gels Detergent Removal Diagnostics DNA Binding Assays Drug Delivery Drug Entrapment Studies Dye Removal Electrophoresis Enzyme Replacement Therapy Extraction from Gels Gene Therapy Glycoprotein/Glycopeptide Purification Immunoblotting In Vitro Cell-Liposome Interaction Studies Ligand Binding Assays...
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sample preparation Technology Selection Guide Chromatography Methods Dialysis (i.e., Tip Columns, PrepTips, ProTips and Spin Columns) A physical separation Electro Dialysis A physical separation method in which small method in which the Physical separation methods, such as: molecules pass through movement of the the pores of a size molecules through a Hydrophilic - Molecules are bound to a functional group on a selective membrane semi-permeable matrix in the organic phase and eluted in the aqueous phase; while the larger membrane is Hydrophobic - Molecules are bound to a functional group on a...
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sample preparation Guide to Sample Preparation Products Ultra-Micro PrepTip™ (U.S. Patent 6,537.502) Ultra-Micro/Micro/Macro ProTip™ Single-Use Disposable Chromatographic Products Tips Ultra-Micro PrepTip™ Ultra-Micro ProTip™ A revolutionary fritless chromatographic device. Does separation without clogging, see page 12-14 Sample Volumes (µl) Micro ProTip™ Macro ProTip™ Contain a hydrophilic polymer material which binds 5 to 10x more protein than silica, see page 15 Hydrophobic (particle size 15-20 microns) C18 Hydrophillic Silica CN NH2 PVOH* PHEA** Ion exchange (Sepharose): Q= Quaternary Amine;...
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sample preparation Guide to Sample Preparation Products Ultra-Micro/Micro TipColumn™ Ultra-Micro/Micro SpinColumn™ Macro SpinColumn™ Single-Use Disposable Chromatographic Products Tip Columns Ultra-Micro Micro Ultra-Micro Micro Macro 384-Well TipColumn™ TipColumn™ SpinColumn™ SpinColumn™ SpinColumn™ SpinColumn™ A wide selection of pre-packed chromatographic materials in pipette tip formats, see page 16-17 Sample Volumes (µl) A wide selection of pre-packed chromatographic materials in SpinColumn formats, see pages 18-20 & 22 96-Well & 96-Well Macro Micro SpinColumn™ SpinColumn™ A 96- or 384-well...
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sample preparation Guide to Sample Preparation Products SpinDIALYZER™/ Fast SpinDIALYZER™ Ultra-Fast DIALYZER™ Flow-Thru DIALYZER™ DIALYSIS Products Reusable DIALYSIS Chambers DIALYZER™ Ultra-Fast DIALYZER™ Drop-in dialysis chamber. 10 Available chamber sizes, see page 26 Drop-in two-sided dialysis chamber. 8 Available chamber sizes, see page 28 Drop-in dialysis chamber with built-in magnet. 8 Available chamber sizes, see page 30 Drop-in two-sided dialysis chamber with built-in magnet. 6 Available chamber sizes, see page 30 Continuous flow, high surface area, in-line dialysis system. 5 Available...
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sample preparation Guide to Sample Preparation Products Ultra-Micro DispoDIALYZER™ Micro DispoDIALYZER™ Fast Macro DispoDIALYZER™ Ultra-Fast DIALYZER for Electroprep™ System DIALYSIS Products Single Use DIALYSIS Products Reusable Chambers for Electroprep Electroprep™ System Fast Flow-Thru DIALYZER™ Drop-in two-sided dialysis chamber with built-in magnet. 6 Available chamber sizes, see page 38 Capable of separating by size and change. 6 Available chamber sizes, see pages 42-45 Continuous flow, single chambered dialysis system. 4 Available chamber sizes, see page 47 Ultra-Micro Micro Fast Macro...
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sample preparation Guide to Sample Preparation Products Fast Micro Equilibrium DIALYZER™ Multi-Equilibrium DIALYZER™ Dispo Equilibrium DIALYZER™ Equilibrium DIALYSIS Products for binding studies, protein-protein interactions and protein-drug binding assays Reusable Products Fast Micro-Equilibrium DIALYZER™ Single-use Products Multi-Equilibrium DIALYZER™ DispoEquilibrium DIALYZER™ A single sample re-usable Up to 20 parallel equilibrium ZSingle sample, single use Single use product for 96 equilibrium dialysis 2-chamber assays. Re-usable chambers product for interaction studies. simultaneous assyas....
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- Automation software solution
- Analysis software solution
- Liquids analyzer
- Rack system
- Windows software
- Automatic analyser
- Control software
- Solids analyser
- Measurement software
- Laboratory analyzer
- Development software
- Spectrophotometer
- Analyzer for the food industry
- Data acquisition software
- Visible spectrophotometer
- UV spectrophotometer
- Modular rack system
- Hydrometer
- Mobile rack system
- Laboratory software