Harvard Apparatus Sample Preparation Catalog
82Pages

Sample Preparation Products Packed Pipette Tips SpinColumns™ Filtration Products DIALYZERS™ Liposomes CoZap™ ElectroPrep™ System Equilibrium DIALYZERS™ CPK® Atomic Models Call to receive other catalogs of interest: Cell Biology & Electrophysiology Physiology & Pharmacology Behavioral Research Micro/Nano Fluidics Electroporation & Electrofusion Molecular Sample Preparation

table of contents Sample Preparation Table of Contents Item Introduction Guides: Applications, Technology & Products Packed Pipette Tips PrepTips™ ProTips™ TipColumns™ SpinColumns™ Single SpinColumns 96-Well and 384-Well SpinColumns Filtration Products Re-Usable DAILYZERS™ DIALYZERS Ultra-Fast DIALYZERS Spin DIALYZERS Flow-Thru DIALYZERS Single Use DIALYZERS™ Ultra-Micro DispoDIALYZERS Micro DispoDIALYZERS Fast Macro DispoDIALYZERS 96-Well DispoDIALYZERS Liposomes CoZap™ 41 ElectroPrep™ System Re-Usable Equilibrium DIALYZERS™ Fast Micro-Equilibrium DIALYZERS Multi-Equilibrium DIALYZERS...

The Sample Preparation Target materials in biological samples are commonly present in trace amounts among larger quantities of numerous impurities or components such as: • Polyacrylamide Gels Excess Dyes Small undesired molecules like primers, peptides, inhibitors & drugs There is currently no single method of purification that is applicable to all samples. Appropriate sample preparation methods are used depending on the type of target material and the sample composition. These impurities or components may have to be completely removed before analysis. Some target materials must also be...

sample preparation Application Guide for Sample Preparation of Biological Mixtures i.e. Proteins, Nucleic Acids, Carbohydrates Acrylamide Removal Affinity Purification Antibody Production Buffer Exchange Cancer Therapy Carbohydrate Purification Cell-Cell Interactions Cosmetics CsCI Removal Destaining Gels Detergent Removal Diagnostics DNA Binding Assays Drug Delivery Drug Entrapment Studies Dye Removal Electrophoresis Enzyme Replacement Therapy Extraction from Gels Gene Therapy Glycoprotein/Glycopeptide Purification Immunoblotting In Vitro Cell-Liposome Interaction Studies Ligand Binding...

sample preparation Technology Selection Guide Chromatography Methods Dialysis (i.e., Tip Columns, PrepTips, ProTips and Spin Columns) A physical separation Electro Dialysis A physical separation method in which small method in which the Physical separation methods, such as: molecules pass through movement of the the pores of a size molecules through a Hydrophilic - Molecules are bound to a functional group on a selective membrane semi-permeable matrix in the organic phase and eluted in the aqueous phase; while the larger membrane is Hydrophobic - Molecules are bound to a functional group on...

sample preparation Guide to Sample Preparation Products Ultra-Micro PrepTip™ (U.S. Patent 6,537.502) Ultra-Micro/Micro/Macro ProTip™ Single-Use Disposable Chromatographic Products Tips Ultra-Micro PrepTip™ Ultra-Micro ProTip™ A revolutionary fritless chromatographic device. Does separation without clogging, see page 12-14 Sample Volumes (µl) Micro ProTip™ Macro ProTip™ Contain a hydrophilic polymer material which binds 5 to 10x more protein than silica, see page 15 Hydrophobic (particle size 15-20 microns) C18 Hydrophillic Silica CN NH2 PVOH* PHEA** Ion exchange (Sepharose): Q= Quaternary...

sample preparation Guide to Sample Preparation Products Ultra-Micro/Micro TipColumn™ Ultra-Micro/Micro SpinColumn™ Macro SpinColumn™ Single-Use Disposable Chromatographic Products Tip Columns Ultra-Micro Micro Ultra-Micro Micro Macro 384-Well TipColumn™ TipColumn™ SpinColumn™ SpinColumn™ SpinColumn™ SpinColumn™ A wide selection of pre-packed chromatographic materials in pipette tip formats, see page 16-17 Sample Volumes (µl) A wide selection of pre-packed chromatographic materials in SpinColumn formats, see pages 18-20 & 22 96-Well & 96-Well Macro Micro SpinColumn™ SpinColumn™ A 96- or...

sample preparation Guide to Sample Preparation Products SpinDIALYZER™/ Fast SpinDIALYZER™ Ultra-Fast DIALYZER™ Flow-Thru DIALYZER™ DIALYSIS Products Reusable DIALYSIS Chambers DIALYZER™ Ultra-Fast DIALYZER™ Drop-in dialysis chamber. 10 Available chamber sizes, see page 26 Drop-in two-sided dialysis chamber. 8 Available chamber sizes, see page 28 Drop-in dialysis chamber with built-in magnet. 8 Available chamber sizes, see page 30 Drop-in two-sided dialysis chamber with built-in magnet. 6 Available chamber sizes, see page 30 Continuous flow, high surface area, in-line dialysis system. 5...

sample preparation Guide to Sample Preparation Products Ultra-Micro DispoDIALYZER™ Micro DispoDIALYZER™ Fast Macro DispoDIALYZER™ Ultra-Fast DIALYZER for Electroprep™ System DIALYSIS Products Single Use DIALYSIS Products Reusable Chambers for Electroprep Electroprep™ System Fast Flow-Thru DIALYZER™ Drop-in two-sided dialysis chamber with built-in magnet. 6 Available chamber sizes, see page 38 Capable of separating by size and change. 6 Available chamber sizes, see pages 42-45 Continuous flow, single chambered dialysis system. 4 Available chamber sizes, see page 47 Ultra-Micro Micro Fast...

sample preparation Guide to Sample Preparation Products Fast Micro Equilibrium DIALYZER™ Multi-Equilibrium DIALYZER™ Dispo Equilibrium DIALYZER™ Equilibrium DIALYSIS Products for binding studies, protein-protein interactions and protein-drug binding assays Reusable Products Fast Micro-Equilibrium DIALYZER™ Single-use Products Multi-Equilibrium DIALYZER™ DispoEquilibrium DIALYZER™ A single sample re-usable Up to 20 parallel equilibrium ZSingle sample, single use Single use product for 96 equilibrium dialysis 2-chamber assays. Re-usable chambers product for interaction studies. simultaneous...