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Harvard Apparatus | Guide to Microfluidic & Nanofluidic Products phone 508.893.8999 • email [email protected] • web www.harvardapparatus.com

Micro/Nanofluidics Introduction Why Miniaturization? Volume of Is a Cube of # of Molecules Diffusion Time (diffusion controlled) Source: A. Manz, ISAS Dortmun 10 Fold Miniaturization 100 x faster separation 100 x capillarity 1000 x smaller volume 10 x lower reagent consumption The two charts above illustrate why there is so much interest in Micro and Nanofluidics: Reduced Sample Size: Researchers can use expensive or precious reagents in small quantity, reducing expense and allowing for increased research on trace amounts of sample. Decreased Scale of Experimentation: Experiments are easier to...

Micro/Nanofluidics Introduction Evolution of Research Reactor Small Technicon Continuous Flow Sample Size Flow Rate Harvard Apparatus | Guide to Microfluidic & Nanofluidic Products Both Batch and Continuous Flow phone 508.893.8999 • email [email protected] • web www.harvardapparatus.com

Micro/Nanofluidics Introduction Fluidic Devices Lab-on-a-chip Sensors Harvard Apparatus | Guide to Microfluidic & Nanofluidic Products Flow Injection Analysis Continuous Flow Batch Reactors Glassware Reactors Years phone 508.893.8999 • email [email protected] • web www.harvardapparatus.com

Micro/Nanofluidics Introduction What do we mean by Micro and Nanofluidics? Harvard Apparatus | Guide to Microfluidic & Nanofluidic Products Photo by Dietrich Kohlheyer of the University of Twente The science of micro and nanofluidics is the building of miniaturized devices with chambers and tunnels for the containment and flow of fluids. With devices measured at the micrometer level and fluids measured in nanoliters and picoliters, microfluidic and nanofluidic devices are widely used in the applications shown in this guide (pgs 8 & 9). phone 508.893.8999 • email [email protected]...

Micro/Nanofluidics Introduction Nanotechnology provides: (10 ) length scale is on the order of a micron. Liquid micro systems and components differ from macro systems primarily due to device fabrication of miniature systems. • Exquisite new tools to engineer novel materials and devices at the nanoscale level, A nanometer, one billionth of a meter, is about 10,000 times narrower than a human hair Nanofluidics: (10-9) The science of building microminiaturized devices with chambers and tunnels for the containment and flow of fluids measured at the nanometer level. Harvard Apparatus 108 years of...

Micro/Nanofluidics Introduction Applications of Microfluidics BIOCHEMISTRY • Enzymatic assays • The Polymerase chain reaction • Nucleic acid arrays • Biomolecular separations • Immunohybridization reactions • Piercing structures for DNA injection Harvard Apparatus | Guide to Microfluidic & Nanofluidic Products CELL BIOLOGY • Flow cytometry/sorting • Sperm/embryo tools: sperm motility, in vitro fertilization, embryo branding • Force measurements with bending cantilevers • Dialectrophoresis/electroporation • Impedance monitoring for cell motility and micromotion • Chemical/physical substrate patterning...

Micro/Nanofluidics Introduction Applications of Nanofluidics BIOLOGY Physiology (filtration, superhydrophobicity, biolubrication, ion channels, actin/myosin, aquaporins) (single molecule studies) Colloid Chemistry (CFD, cavitation, slip flow) (DLVO theory, electrokinetics, Donnan equil,. semipermeability) NANOFLUIDICS Polymer Science Surface Science Separation Science (chromatographic packings) (brush layers) Membrane Science (nanoporosity, size exclusion, semipermeability) Soil Science (water potential, zeolites) ENGINEERING Microengineering (stiction, device fabrication) Harvard Apparatus |...

Harvard Apparatus | Guide to Microfluidic & Nanofluidic Products 108 Years of Experience in Low Flow Science Harvard Apparatus offers superior solutions for the challenges of microfluidic and nanofluidic applications. Harvard Apparatus’ 108 years of experience in low-flow science provides you low flow (microliter, nanoliter, picoliter and femtoliter) fluid delivery systems with high accuracy and precision, pulse free pumping mechanisms and critical accessories for microfluidics and nanofluidics. Harvard Apparatus offers singleand multi-channel volume and pressure controlled pumps with flow rates...

I I A p\/A D Harvard Apparatus utilizes a four part n**r\V^r\Ly approach to solve the challenges of APPARATUS Micro and Nanofluidics! SUPPORT TEAM MICRO & NANO • ADVANCED FAMILY OF LOW-FLOW PUMPING PRODUCTS • BROADEST RANGE OF ACCESSORIES • EXPERTS TO ASSIST YOU IN YOUR SELECTION • SPECIALIZED FLUIDICS PRODUCTS Innovative technology • 108 years low flow fluidics expertise • Varied product offerings customized for your fluidic requirements phone 508.893.8999 • email [email protected] • web www.harvardapparatus.com

Let Our Expertise Work for You Harvard Apparatus…Family of Fluidic Solutions Companies from Nano to Micro • SYRINGE & PRESSURE PUMPS • CONNECTORS • GLASS CAPILLARIES Harvard Apparatus | Guide to Microfluidic & Nanofluidic Products Syringe Pumps Pressure Pumps • PERFUSION MANIFOLDS • DATA ACQUISITION • BIOSENSORS • TRANSDUCERS HAS-HA Lung Slice Chamber • ELECTRODES Pipes • AMPLIFIERS • IMAGING CHAMBERS • PERFUSION MANIFOLDS • DATA ACQUISITION • BIOSENSORS • TRANSDUCERS • AMPLIFIERS Cell Imaging Chamber • PNEUMATIC INJECTORS • PULLERS phone 508.893.8999 • email [email protected]...

Let Our Expertise Work for You If you wish to receive some assistance, please have the answers to the following questions and we will help you select the appropriate pump for your application. 1. Please describe your application?____________________________________________________________ ______________________________________________________________________________________________ Do you need to infuse only or infuse/withdraw?__________ What volume do you want to infuse or withdraw?__________ What size syringes are you working with?__________ What flow rates are you working with?__________ How...