HARVARD APPARATUS Exploring Tissue Chemistry Head Office, Sweden Box 2, 171 18, Solna, Sweden Tel: +46-8-470 10 00 • Fax +46-8-470 10 50 E-mail: [email protected] Harvard Apparatus US Office: 84 October Hill Road Holliston • MA 01746 • USA Tel: 508-893-8999 • Fax 508-429-5732 E-mail: [email protected] products for MICRODIALYSIS research www.microdialysis.com www.harvardapparatus.com MICRODIALYSIS research

Table of Contents Introduction Minor Surgical Kit Major Deluxe Surgical Kit Rat Surgical Kit Mouse Surgical Kit Microdialysis for Basic Research The Principle of Mi crodialysis Microdialysis Academy Microdialysis Academy Probes Overview CMA 7 Microdialysis Probe CMA 11 Microdialysis Probe CMA 12 Microdialysis Probe CMA 20 Microdialysis Probe CMA 30 Linear Microdialysis Probe CMA 31 Linear Microdialysis Probe Probe Guide Cannulae Custom Made Probes Accessories - Probes Instruments CMA 120 System for Freely Moving Animals Mouse Infusion Cage Harvard/Instech Counter Balanced Lever Arms Harvard/Instech...

Exploring Tissue Chemistry! The Company CMA Microdialysis is a Swedish life science research tools company devoted to the development, manufacturing, and marketing of the microdialysis technique. The company was formed in 1984 as the rst company in the world to market microdialysis products and know-how. Instruments and consumables are sold globally to universities and pharmaceutical companies as unique tools for in vivo sampling and monitoring of organs and tissues. CMA is the market leader in the development and distribution of microdialysis products for scientic research. With a highly specialized...

Introduction Products CMA develops and produces a complete range of microdialysis products. The basic research portfolio includes a broad range of probes, pumps, fraction collectors and complete systems for research. Research Applications For more than thirty years, microdialysis has been used to study brain neurophysiology and the release of neurotransmitters, monoamines, and metabolites, amino acids and other small endogenous compounds. With the introduction of several new microdialysis probes for use in the peripheral organs, microdialysis is seeing widespread use in sampling molecules in...

Introduction CMA’s basic research solutions are used by leading pharmaceutical companies in the drug discovery process to assess drug concentrations at the site of drug action. Since the exchange of molecules through the dialysis membrane is in both directions, a microdialysis probe placed in target tissue can be used to continuously: Sample unbound drug and/or active metabolites as they arrive to the tissue following systemic administration. Deliver drug locally into an organ or tissue through the probe, and simultaneously collect endogenous target compounds to determine pharmacological effect....

Microdialysis for Basic Research Microdialysis Probes Microdialysis Instruments Surgical Instruments

Microdialysis for Basic Research 7 The Principle of Microdialysis Microdialysis is a technique used to monitor the chemistry of the extracellular space in living tissue. Microdialysis gives you a preview of what goes on in tissues, before chemical events can be reected as changes in systemic blood levels. The microdialysis probe is designed to mimic a blood capillary and by keeping this metaphor in mind, it is easy to conceive of the many ways you can use this technique. Following implantation into a tissue, a physiological salt solution is slowly pumped through the microdialysis probe. In the...

Microdialysis for Basic Research 8 Microdialysis Academy Design your own microdialysis experiment The following is a simple checklist to help you design your own microdialysis experiment: 1. PROPERTIES OF THE PROBE MEMBRANE A membrane with a low molecular weight cut off puries your sample by excluding large molecules, while a high molecular cut off recovers some larger substances, such as peptides or proteins. 6. TIME NEEDED TO OBTAIN STEADY STATE CONDITIONS The introduction of a probe into the tissue will always cause damage and the recovery of function will take a certain period of time. An...

Microdialysis for Basic Research Microdialysis Systems and Congurations Overview THERE ARE A FEW DIFFERENT MODELS of each component in the Microdialysis system and each is described in this catalog. Your choice of components, or system conguration, should be based on the application and the experimental requirements. After deciding to work with anesthetized or freely moving animals, take a look at a few of the more common congurations below, from the basic system with a two-syringe pump and 20-sample fraction collector, to the four-syringe pump with 64-sample refrigerated fraction collector....

Microdialysis for Basic Research Microdialysis Systems and Congurations Overview a CMA 470 Refrigerated Fraction Collector Stereotaxic Instrument CMA 402 Syringe Pump Micro T MdCAD software Stereotaxic Instrument Microdialysis Systems for Freely Moving Animals a CMA 470 Refrigerated Fraction Collector CMA 120 System for Freely Moving Animals (incl. Bowl, Swivel Assembly and Balance Arm) Instrument Table

Microdialysis for Basic Research Microdialysis Systems and Congurations Overview CMA 400 Syringe Pump CMA 120 System for Freely Moving Animals (incl. Bowl, Swivel Assembly and Balance Arm) Instrument Table CMA 470 Refrigerated Fraction Collector CMA 120 System for Freely Moving Animals (incl. Bowl, Swivel Assembly and Balance Arm) Instrument Table CMA 120 System for Freely Moving Animals (incl. Bowl, Swivel Assembly and Balance Arm) Instrument Table

Probes Microdialysis for Basic Research Microdialysis Probes ALL CMA MICRODIALYSIS PROBES have the same concentric construction except the linear probes. An inner cannula leads the perfusion fluid from the inlet to the tip of the probe where it comes in contact with the semi-permeable membrane. The fluid then continues up through the outer shaft on its way to the outlet. All metal parts are treated to prevent oxidation of labile compounds in the perfusate. A variety of probe types, sizes, and membranes are described over the next few pages. Probes are delivered in vacuum-sealed packages, 3 pcs/pkg,...