BTX Electroporation & Transfection Catalog
27Pages

HARVARD APPARATUS Bacteria & Yeast Plants & Insects Mammalian Cell

www.btxonline.com, here are a few reasons why… • Register for newsletters and technical updates • Contact us for technical support or a quotation • View a complete listing of BTX distributors worldwide • Search our products • Submit protocols • To Order, call Toll-Free 1.800.272.2775 or 1.508.893.8999 800.272.2775 (USA & Canada) phone 508.893.8999 fax 508.429.5732 toll-free customer/technical support e-mail and website:

Technology Electroporation The Complete Electroporation & Electrofusion Catalog BTX has been at the forefront of electroporation technology since we introduced the first commercially available electroporator in 1983. For the past 26 years we have made it our priority to focus only on Electroporation and Electrofusion. This focus allowed us to develop the experience and expertise to supply you, the researchers, with the broadest selection of innovative in vivo or in vitro tools. These tools allow you to advance your research. BTX offers a diverse number of possible solutions, putting you in...

NEW! BTXpress™ High Performance Electroporation Solution See page 30-31 for details. ECM® 630 High Throughput HT Multi-Well Electroporation System NEW! MicroJect 1000 Max System See page 27 for details. toll free NEW! Petri Dish Platinum Electrode for Tissue Slices See page 42 for details. toll free

Electroporation has long been recognized as one of the most efficient methods of transforming human genes into prokaryotic cell lines. Researchers use this technique to express recombinant proteins to study gene function and for the therapeutic treatment of human diseases. Typically, the most commonly transformed cell lines are bacteria and yeast, such as Escheria coli, Agrobacterium Tumerfaciens, Pichia Pastoris and Saccharomyces Cerevisiae. Electroporation of these gram-negative bacterial strains can achieve transformation success rates in the range of 1X1010 transformants/ug DNA....

For many years, Agriculture and Horticulture labs have used electroporation to transform plants in order to generate transgenic crops (GMO). Electroporation offers a an alternative method for plants & insects the delivery of genes directly into plant cells, plant tissues and plant protoplasts. Electrofusion allows for the fusion of plant protoplasts for transgenic modified plant applications. Transformation of plants can be successfully accomplished without prior removal of the cell wall, allowing for greater genetic manipulation potential of the plant cells. Whether performing a stable...

Electroporation is an efficient non-viral method used to transfect genes and other molecules into mammalian cell lines. This technology is commonly used to study gene targeting, function and mammalian cells to understand protein regulation. Electroporation is a standard method used to transfect mammalian cell lines to express recombinant human proteins which are used for therapeutic purposes. Gene delivery by this method is typically used to create a transient transfection in order to study protein expression or to temporarily knockout or “silence” these genes using siRNA; which is used to...

In Vivo, In Utero & In Ovo Applications equipment The delivery of genes and drugs directly into living tissues has significant implications in gene therapy applications, cancer treatments, vaccine development and transgenic animal e production. Tissues and whole embryos can be transfected with the use of specialty electrodes for the following methods; in vivo, in ovo, in utero and ex vivo tissues. Electroporation mediated gene and drug delivery has been shown to substantially increase intracellular uptake and expression of DNA, siRNA and miRNA in a variety of tissue such as muscle, skin,...

Electrofusion, Mam malian, Oocyte & Plant equipment Electrofusion is a highly efficient, reproducible and non-toxic technique used in a wide variety of applications. The number of applications requiring the use of electrofusion has greatly increased electrofusion in the past decade and include; hybridoma productions for antibody expression, stem cell development, genetically modified plant protoplast, tetraploid fusion and nuclear transfer for transgenic development. Many applications have clinical significance in diagnostic testing, therapeutics and vaccine development. The ECM 2001 is a...

Crossover Guide Microinjection is a well established technique used routinely for injection of animal cells, tissues and embryos to insert genetic material such as DNA, RNA, proteins and macromolecules microinjection directly into animal cells or embryos a cell cytoplasm or nucleus. Genetic material is inserted via a needle varying in size from 0.5uM to 20uM in diameter. The needle penetrates the cell membrane and/or the nuclear envelope. The process is performed, observed and controlled by using a micromanipulator specialized microscope set up. This technique is widely used by researchers...

Decision Guide Electroporation Definitions Guide Brain Tissue or Brain Slice Tissue Slice Chamber/Tissue Chamber/ L-Shaped Needle Tissue Slice Chamber/Tissue Chamber/ Genepaddles Developmental Biology Tissue Chamber/Tweezertrodes/ Genetrodes Cell Biology Ophthalmology Cancer Research Gene Therapy Transfection of delicate brain tissue and tissues with unique morphologies are more easily transfected with our new tissue slice chamber, L-shaped platinum needles or other BTX electrodes. Developmental Biology Neuroscience Neurology Embryology L-Shaped Needle/Tweezertrodes/ Genepaddles Cancer...

General Optimiza tion Guide Electrofusion is an expansion of electroporation using different buffers and one or more proprietory alternating current (AC) pulse(s). Cells are brought together or “aligned” by the use of an AC pulse which causes charges to form on the cellular membrane (dielectrophoresis) resulting in alignment of cells or pearl-chain (dimer) formation. Following the AC cellular alignment the DC pulse is applied to induce permeation of the cell membrane. When cells are brought into contact during electroporation, these cells are induced to fuse. Following this DC pulse the AC...