Ultra-low readout noise Fast frame S E N O I rates and back-thin boosted high See what you’ve been missing. Capture visually stunning high S/N images from Resolve high-speed temporal events. Computationally analyze images with confidence.

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Back-thin Boosted QE for Maximum Photon Collection 100 QUANTUM EFFICIENCY (%) Minimal and Highly Uniform Readout Noise Results in Maximum Low Light Detection 1,000,000 (Ultra-quiet) (Standard) (Fast) ORCA-Fusion BT: Detection Optimized The ORCA-Fusion represented a leap forward from “Gen II” sCMOS, providing significant advantages in readout noise and uniformity for improved image quality. The ORCA-Fusion BT is the natural technological progression. The unique combination of back-thin boosted QE plus low noise and high uniformity delivers the ultimate in camera performance, versatility and sensitivity...

1. Mouse kidney imaged on a 3i Cleared Tissue LightSheet System with ORCA-Fusion BT. Sample courtesy of Dr. Bo Shen, University of Texas Southwestern Medical Center. 2. Frontal view of whole mouse brain labeled with Thy1-GFP imaged on a 3i Cleared Tissue LightSheet System with ORCA-Fusion BT. Sample courtesy of Dr. Hu Zhao, Texas A&M College of Dentistry. 3. Lateral view of whole mouse brain labeled with Thy1-GFP imaged on a 3i Cleared Tissue LightSheet System with ORCA-Fusion BT. Sample courtesy of Dr. Hu Zhao, Texas A&M College of Dentistry. 4. Brainstem neurons in whole mouse brain labeled...

Super resolution image of fixed Cos7 cells labeled with Tubulin-AF647. Image acquired using Olympus microscope IX83 with 100x TIRF 1.50 and ZDC 2 + Abbelight SAFe 360 with ORCA-Fusion BT camera for a FOV of 85 x 85 µm2. Localization precision of 10nm/10nm/15nm (x,y,z). Image courtesy of Abbelight. Frames per second (fps) READOUT MODE AREA READOUT MODE Scan mode Fast scan LIGHTSHEET READOUT MODE Standard scan Ultra-quiet scan Fast scan *1 Faster frame rates achievable at 8 and 12 bit. Typical FPS with 2×2 binning READOUT MODE AREA READOUT MODE LIGHTSHEET READOUT MODE Fast scan Scan mode Standard...

Custom designed with research in mind L I V E - C E L L No one camera can delivery perfect performance for every application. But the ORCA-Fusion BT comes close. This custom sensor was created to make the most L O W - L demanding imaging applications easy. Few photons? No problem. Fast live cell I G H T events? Turn up the speed. Require reliable raw images for quantitative analysis? Compute with individualized camera noise calibration data. W I D E - F I E L D F L U O R E S C E N C E M I C R O S C O P Y Q U A N T I T A T I V E A E T R has K I N tell M O L E C U L dim sampleA C secrets toG but...

Read noise *1 Fast scan Standard scan Ultra-quiet scan 1.6 electrons, rms 1.0 electrons, rms 0.7 electrons, rms Full well capacity *1 Dynamic range *1, *2, *4 Conversion factor *1, *2 Cooling Temperature With forced-air Water cooled Water cooled (Max cooling) -8 ˚C (Ambient temperature: +25 ˚C) -8 ˚C (Water temperature: +25 ˚C) Less than -15 ˚C (Ambient temperature: +20 ˚C, water temperature: +20 ˚C) Dark current *1, *3 @ -8 ˚C @ -15 ˚C Dark offset 1.0 electrons/pixel/second 0.7 electrons/pixel/second 100 counts Typical value Dark signal non-uniformity (DSNU) *1,*4 0.06 electrons rms in Ultra-quiet...

CAMERA SPECS LOW N O I S E A N D E XC E PT I O N A L READOUT NOISE UNIFORMITY WITH HIGH QE Ultra-quiet Scan HIGH SPEED HIGH RESOLUTION PIXEL SIZE DYNAMIC RANGE Ultra-quiet Scan Ultra-quiet Scan HAMAMATSU PHOTONICSBoostedwww.hamamatsu.com Photon Collection Back-thin K.K. QE for Maximum Systems Division 100 812 Joko-cho, Higashi-ku, Hamamatsu City, 431-3196, Japan, Telephone: (81)53-431-0124, Fax: (81)53-433-8031, E-mail: [email protected] QUANTUM EFFICIENCY (%) U.S.A.: Hamamatsu Corporation: 360 Foothill Road, Bridgewater, NJ 08807, U.S.A., Telephone: (1)908-231-0960, Fax: (1)908-231-1218 E-mail:...