HAMAMATSU PHOTONICS K.K., Systems Division 812 Joko-cho, Higashi-ku, Hamamatsu City, 431-3196, Japan, Telephone: (81)53-431-0124, Fax: (81)53-435-1574, E-mail: [email protected] U.S.A.: Hamamatsu Corporation: 360 Foothill Road, Bridgewater, NJ 08807, U.S.A., Telephone: (1)908-231-0960, Fax: (1)908-231-1218 E-mail: [email protected] Germany: Hamamatsu Photonics Deutschland GmbH.: Arzbergerstr. 10, D-82211 Herrsching am Ammersee, Germany, Telephone: (49)8152-375-0, Fax: (49)8152-265-8 E-mail: [email protected] France: Hamamatsu Photonics France S.A.R.L.: 19, Rue du Saule Trapu, Parc du Moulin de Massy, 91882 Massy Cedex, France, Telephone: (33)1 69 53 71 00, Fax: (33)1 69 53 71 10 E-mail: [email protected] United Kingdom: Hamamatsu Photonics UK Limited: 2 Howard Court,10 Tewin Road, Welwyn Garden City, Hertfordshire AL7 1BW, UK, Telephone: (44)1707-294888, Fax: (44)1707-325777 E-mail: [email protected] North Europe: Hamamatsu Photonics Norden AB: Torshamnsgatan 35 16440 Kista, Sweden, Telephone: (46)8-509-031-00, Fax: (46)8-509-031-01 E-mail: [email protected] Italy: Hamamatsu Photonics Italia S.r.l.: Strada della Moia, 1 int. 6, 20020 Arese (Milano), Italy, Telephone: (39)02-935-81-733, Fax: (39)02-935-81-741 E-mail: [email protected] China: Hamamatsu Photonics (China) Co., Ltd.: 1201 Tower B, Jiaming Center, 27 Dongsanhuan Beilu, Chaoyang District, 100020 Beijing, China, Telephone: (86)10-6586-6006, Fax: (86)10-6586-2866 E-mail: [email protected] Taiwan: Hamamatsu Photonics Taiwan Co., Ltd.: 8F-3, No.158, Section2, Gongdao 5th Road, East District, Hsinchu, 300, Taiwan R.O.C. Telephone: (886)03-659-0080, Fax: (886)07-811-7238 E-mail: [email protected] We’ve advanced our camera technology, so you can advance your science Building on our extensive experience with high performance scientific cameras and advanced imaging applications, Hamamatsu introduces the new ORCA-Flash4.0 V3. This one camera expertly handles applications ranging from the acquisition of beautiful scientific images to experiments that demand detection, quantification and speed. With on-board FPGA processing enabling intelligent data reduction, highly refined in-camera, pixel-level calibrations, increased USB 3.0 frame rates, purposeful and innovative triggering capabilities, patented lightsheet read out modes and individual camera noise characterization the ORCA-Flash4.0 V3 is the precision instrument for imaging.

Calibrated for Quantitative Accuracy Our ORCA-Flash4.0 cameras have always provided the advantage of low camera noise. In quantitative applications, like single molecule imaging and super resolution microscopy imaging, fully understanding camera noise is also important. Every ORCA-Flash4.0 V3 is carefully calibrated... as a precision instrument must be. Our attention to this detail delivers outstanding linearity, especially at low light, and offers improved photo response non-uniformity (PRNU) and dark signal non-uniformity (DSNU) to minimize pixel differences and reduce fixed pattern noise....

Focus on the Relevant Data Our new “Enhanced Visualization Mode” was designed to help answer the question “Can I see it?” Many low light experiments, especially those that were previously imaged using EM-CCDs are now routinely accomplished—with better signal to noise, faster speeds and at far less expense—by sCMOS cameras. However, we sometimes miss the visual punchiness of EM-CCD images. Enhanced Visualization Mode pops the contrast of the displayed image, while saving the sCMOS raw image data to disk. And sometimes our innate tendency to seek contrast can be a distraction. Even a few hot pixels...

Pixel Array (horizontal by vertical) Effective Area (horizontal by vertical in mm) Peak Quantum Efficiency (QE) Dynamic Range1 Readout Noise (Nr) median in electrons slow scan1 Readout Noise (Nr) rms in electrons slow scan1 Readout Noise (Nr) median in electrons standard scan1 Readout Noise (Nr) rms in electrons standard scan1 Maximum Full Resolution Frame Rate (fps) Cooling Temperature Readout Dark Current (electrons/pixel/s) – Air Cooled to -10° C Dark Current (electrons/pixel/s) – Water Cooled to -10° C Dark Current (electrons/pixel/s) – Water Cooled to -30° C Full Well Capacity in electrons1...