Substrate that enhances the weak Raman scattered light from the molecules The surface-enhanced Raman spectroscopy (SERS) substrate enhances the Raman scattering light from molecules, making high-sensitive Raman spectroscopic analysis possible. A chip of metallic nano/sub-micro structure is mounted on Hamamatsu’s original handling plate to protect its active area. The active area of the handling plate is of a well structure for easily attaching solution or the like. It is noted this is a disposable product and cannot be reused. Uniform activated surface structure Chemical analysis (e.g., molecule type and structure) Handling plate that protects the active area Environmental measurement (e.g., water quality, pesticides, toxic substance) Well structure to facilitate attaching solution or the like Biological measurements (biomolecules of protein, saccharides, etc.) SERS SERS is a method for enhancing Raman scattering light (Raman scattering light is typically weak.). The scattering light generated on incidence to molecules is mainly scattering light (Rayleigh scattering light) of a wavelength common to that of the incident light. In addition to the Rayleigh scattering light, a faint amount of the scattering light (Raman scattering light) of wavelengths different from that of the incident light is emitted. The difference (Raman shift) in the frequencies between the incident light and the Raman scattering light comes from the natural frequencies of the molecules. Spectrometry of the Raman scattering light reveals information about a type and a structure of the molecule (type of chemical bond, level of crystallization, distortion of crystal lattice, etc.). For example, glucose and galactose are isomers that have nearly the same chemical structures, but they can be distinguished by Raman spectroscopic analysis. The enhancement of the faint Raman scattering light by the SERS substrate facilitates spectrometry. When a minute metal (Au) structure formed by nanoimprint technology is exposed to light, and interaction (surface plasmon resonance) occurs between the light and the electrons of the metal surface, creating an enhanced electric field. This effect enhances the Raman scattering light of the molecules that are close or attached to the metal surface. Incident light Surface-enhanced Raman scattering light Metallic nano/sub-micro structure (Au) KOTHC0080EA Notice ∙ Do not apply shock or pressure anywhere on the substrate. ∙ This is a disposable product and cannot be reused. ∙ Recommended storage temperature: 4 to 25 °C (unopened condition) ∙ The expiration date is indicated on the packing. Do not use the product after the expiration date. Note: The expiration date is 3 months after the shipping date on the packing.

SERS substrate Specifications (typical values) Parameter Substrate size Chip size Active area Well capacity Activated surface structure Handling plate material Raman excitation wavelength (recommended) Specification 76 × 26 × 3.6 4×4 2.7 × 2.7 6 Au nanostructure Polypropylene Dimensional outline (unit: mm) Example of Raman spectrum Active area 2.7 × 2.7 (well structure) * Distance from the top of handling plate to the active area KOTHA0006EA 4,4’-Bipyridyl aqueous solution 10 µM on SERS substrate 4,4’-Bipyridyl aqueous solution 1 mM on glass substrate 400 Information described in this material...