EM-CCD camera Multiply faster The ImagEM X2 is an extremely versatile camera that quietly delivers 70 frames/s at full frame and up to 1076 frames/s with analog binning and regions of interest. With very high signal to noise in near dark conditions and extremely low dark current, the ImagEM X2 enables quantitative ultra-low light imaging both for long integration times and at high speed. With EM gain off, the extremely deep full well capacity can extract information from the lowest contrast bright images. Additional features allow for optimized camera triggering, on-board for EM gain protection, streamlined connectivity through IEEE1394b, improved overall signal to noise and increased non-EM dynamic range. Hamamatsu has taken the beloved 512 × 512 EM-CCD sensor and created a masterfully redesigned camera that delivers maximum speed and precision performance. The ImagEM X2-1K 1024 × 1024 version is also available. High-speed readout (ImagEM X2) 70 frames/s at full resolution at Sub-array 16 pixel, 4×4 binning

The ImagEM X2 expertly handles a wide range of applications from bright light, Hungry for Photons With large pixels, high QE, and relatively zero readout noise, EM-CCD technology performs in low light conditions. How low light? When you've got fewer than 10 photons per pixel between the sample and background, EM-CCDs are the perfect tool for the job, delivering the best SNR of any camera technology. For high mag, biologically relevant applications with routine exposure times of 10 ms to 30 ms, the sample is likely emitting hundreds to thousands of photons per pixel. But with faster speeds come...

low contrast to ultra-low light imaging. Mechanical shutter The ImagEM X2 series includes an integrated mechanical shutter in order to protect the camera from EM gain degradation and to lessen afterimage effects. The mechanical shutter is software controlled. EM gain measurement and calibration Gain aging is a known and expected process in EM technology. Even when every care is taken to minimize gain aging, use of the camera in EM mode, especially with high gains or high intensity light, can degrade the gain. Since this is a use-dependent phenomenon, it's important to know when it's happened...

High Sensitivity Quantum efficiency (%) The beloved EM-CCD sensor provides over 90 % peak QE. High EM gain of maximum 1200× EM gain feature is ideal for live cell imaging because of shorter exposure times and reduced excitation light levels. Comparison of sensitivity with conventional camera: Luminescence imaging of HeLa cells expressing Renilla Luciferase. Conventional cooled CCD camera Minimal dark noise is another benefit of stable cooling performance The dark current of a CCD depends on the temperature, and it decreases by half when the temperature drops by approximately 7 ˚C to 8 ˚C. Therefore,...

Great Stability Highly stabilized EM gain by cooling temperature control Maintaining stable cooling temperature is essential to stable gain settings required for superior performance in long duration imaging and analysis. Very precise control of the cooling temperature in the ImagEM X2 series is a key benefit. Examples of temperature stability and EM gain stability Temperature stability Camera: C9100-23B Clock: 22 MHz Cooling temperature: –65 ˚C Air cool, Ambient temperature: +20 ˚C After warming up, start measurement. Camera: C9100-23B Clock: 22 MHz Cooling temperature: –65 ˚C Air cool, Ambient...

Selectable Readout Modes Select a readout mode for optimal image acquisition based on the sample brightness or desired frame rate or exposure time. EM-CCD readout Normal-CCD readout For short exposure, high sensitivity imaging For high dynamic range imaging Sample of EM-CCD readout Sample of Normal-CCD readout Confocal calcium ion imaging of HeLa cells expressing yellow Chameleon 3.6. This image shows changes of histamine stimulated calcium ion with two Z positions and four time lapse. Luminescence imaging of HeLa cells expressing Renilla Luciferase. Objective lens: UApo/340 20× Exposure time:...

Specifications Type number Camera head type Window AR mask Imaging device Effective number of pixels Pixel size Effective area Pixel clock rate EM-CCD readout Normal-CCD readout EM (electron multiplying) gain (typ.) *2 Ultra-low light detection Fastest readout speed Readout noise EM-CCD EM gain 4× (C9100-23B) (rms) (typ.) readout 10× (C9100-24B) EM gain 1200× C9100-24B (ImagEM X2-1K EM-CCD camera) C9100-23B (ImagEM X2 EM-CCD camera) Hermetic vacuum-sealed chamber air/water-cooled head *1 Anti-reflection (AR) coatings on both sides, single window No Yes Electron Multiplying Back-Thinned Frame...

Configuration example Video camera attachment (C-mount) ImagEM X2 EM-CCD camera set ImagEM X2-1K EM-CCD camera set Computer frame grabber board Water circulator Commercially available software HCImage Live software* * HCImage Live software provides standard image measurement functions. Upgrades to more feature-rich versions are available. Standard Option Please contact your local Hamamatsu representative or distributor regarding actual configuration. Dimensional outlines Options Binning option for C9100-23B: M10354-03 External trigger cable SMA-BNC 5 m: A12106-05 External trigger cable SMA-SMA...