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DIUTHAME
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Ionization-assisting substrates Desorption Ionization Using Through Hole Alumina MEmbrane

DIUTHAME® ensures high reproducibility and accuracy in your mass spectrometry tasks! DIUTHAME is … … an ionization-assisting tool developed by Hamamatsu for MALDI TOF-MS (time-of-flight mass spectrometry). DIUTHAME streamlines the ionization process in mass spectrometry by replacing the matrix used in MALDI, and totally eliminates the cumbersome pretreatment of samples needed up till now. Medical care Drug discovery Basic research Material science Places where DIUTHAME is used Examples of compatible samples Research institutes with MALDI mass spectrometers Compatible with a wide range of...

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No matrix background noise High reproducibility (Measureble at low molecular region) with minimal variation no matter who does High spatial resolution Needs no sample pretreatment, slashes the time and effort nanometer-order structure Measurement using matrix How DIUTHAME differs from matrix ionization Matrix preparation / Apply matrix Measurement using DIUTHAME DIUTHAME is placed on sample *In mass spectrometry imaging Generates matrix-derived noise Background noise (Low molecular region) Generates virtually no noise Depends on worker skill Spatial resolution High resolution due to...

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Mass spectrum measurement examples Amino acid/Peptide mix Measurements were carried out in cooperation with Associate Professor Yasuhide Naito, The Graduate School for the Creation of New Photonics Industries Amino acid Amino acid Alanine A Cysteine C Histidine H Arginine R Tryptophan W W[M+Na]+ C[M+K]+ Measurement sample details Measurement method 1The mixed sample was dropped 1 μL from above the DIUTHAME. Dissolve 5 amino acids in pure water and mix them. ▼ Measurement condition Measuremenr sample Mixture of 5 kinds of amino acids 2Remove the ﬁlm and attach the DIUTHAME substrate onto the...

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Mass spectrum measurement examples Polyethylene glycol 2000 / Polystyrene / Insulin Polyethylene glycol 2000 Measurement method 1The mixed sample was dropped 3 μL from above the DIUTHAME. 2Remove the ﬁlm and attach the DIUTHAME substrate onto the measurement plate. Measurement sample details 1×104 PEG2000(1 mg/mL in ACE): NaTFA(1 mg/mL in ACE)=10: 1 ACE: Acetone 0.5×104 ▼ Measurement condition Measuremenr sample Reﬂectron, positive ion mode Measurement method 1The mixed sample was dropped 3 μL from above the DIUTHAME. 2Remove the ﬁlm and attach the DIUTHAME substrate onto the measurement...

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Mass spectrometry imaging measurement examples Black rice Measurements were carried out in cooperation with Designated Assistant Professor Keiko Kuwata, The Institute of Transformative Bio-Molecules Nagoya University Measurement method Set a slice of black rice on an ITO glass slide. Remove the ﬁlm and place the DIUTHAME on the black rice. Drop 2 μL of “70 % AcCN / 30 % H20” solution from above the DIUTHAME to extract the components of interest. Start measurement after the sample dries. Measurement example Sample Mass spectrometry imaging of black rice * * After taking mass spectrometry...

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Mass spectrometry imaging measurement examples MOuse brain Measurement method Set a slice of frozen mouse brain on an ITO glass slide. Place DIUTHAME on the mouse brain slice before it thaws. After the sample thaws with ﬁngertip heat the components of the sample will soak up to the surface by capillary action. Start measurement after the sample dries. Slice Sample Heat Do this work in a cryostat MS imaging result of one sample taken by positive and negative ion mode Laser irradiation marks on the DIUTHAME * Before making measurements using mass spectrometer, an optical image was captured...

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Measurement example of mass spectrometry imaging using blotting Strawberry Measurement carried in cooperation with Associate Professor Hirofumi Enomoto, The Department of Biosciences Teikyo University Measurement method ▼Image of strawberry ▼Blotting image ▼Optical image Cut an unfrozen Place the DIUTHAME, Allow the DIUTHAME Start measurement after the sample strawberry in half. with its rear side facing to soak up the the cut surface of the strawberry's drie d ies. dries. strawberry to allow w blotting. components. Measurement example 1 e Number Positive ion mode measure ion mode...

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Measurement example of mass spectrometry imaging using blotting Industrial product Measurement method Place the DIUTHAME on the measurement position (where white stains have occurred) of the product. Drop 1 μL of solvent from above the DIUTHAME to transfer the stains. Set the DIUTHAME with the transferred stains on the measurement plate. Start measurement after the sample dries. Measurement example White stains have occurred on an industrial product. Measurement of a large, thick sample using blotting technique ▼ Measurement condition Laser pitch * Area in white dotted line circle shows...

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Dimensional outline For Mass spectrum ● 3 × 9 ch type · A14111-3-1 (For mass spectrum) 27.0 ± 0.2 ADHESIVE SECTION *1 *1 Adhesive section with target plate or ITO glass slide *2 Thickness including conductive tape REAR VIEW

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Dimensional outline For MS imaging ● 18 type · A13331-18-2 · A13331-18-2B (For blotting) EFFECTIVE AREA 1 MIN. (5.5) ●Glass slide size type · A13331-5019-1 · A13331-5019-1B (For blotting)

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Website information To find more detailed information about DIUTHAME, please visit our website via QR code or URL below. [Main contents on website] • Application notes • How-to-use instruction video • Related literature • What the developer has to say • Interview with users https://www.hamamatsu.com/jp/en/product/optical-components/DIUTHAME/index.html * DIUTHAME is registerd trademarks of HAMAMATSU PHOTONICS K.K.. Subject to local technical requirements and regulations, availability of products included in this promotional material may vary. Please consult with our sales office. Information...

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