Dried Blood Spot Autosampler DBSA Specifications Uses The Dried Blood Spot Autosampler DBSA offers complete automation of the sampling steps from DBSA cards, onto which matrix spots have been placed for analysis. DBS sampling can be combined with sample preparation and clean-up steps, such as SPE, and with LC/MS analysis. Time consuming manual sample preparation steps such as spot punching, elution and centrifugation can be eliminated. Sample desorption is performed in a highly reproducible manner, and the defined area of the matrix spot is completely desorbed by means of the patented Flow-Through Desorption (FTD™)† technology. DBSA automation also increases sample throughput since processing of extensive sample series can be performed overnight and over the weekend. System Configuration • Compatible with all common LC systems Technical changes and errors reserved. 022016 • Automation based on a GERSTEL MultiPurpose Sampler MPS with xt-board • Expandable with the GERSTEL SPExos for clean-up and analyte concentration Automated DBS Steps • Identification of the DBS card by means of a bar code • Spot integrity check by means of a camera • Addition of an internal standard • Sample clean-up and analyte concentration using the GERSTEL SPExos (optional) • LC/MS analysis DBS Card Formats • Whatman® FTA® DMPK cards for 4 spots‡ • PerkinElmer-Bioanalysis-Card GR2261004 for 4 spots Desorption Area • Radius of desorbed area user selectable at 2 mm, 4 mm, 6 mm or 8 mm • Full spot or partial spot desorption Desorption Temperature • Solvent temperature AT or 40 … 100 °C Sample Capacity • 24 cards per rack • 2 racks are placed on one rack holder • 1 … 5 rack holders per sampler, depending on the MPS model used • up to 240 cards per DBSA Sample Throughput • With an estimated cycle time of 5 minutes per sample the DBSA can process 96 samples in 8 hours. • Desorption of the sample from a defined area using a defined solvent flow • Verification of successful desorption by means of a camera and image recognition Custom solutions for spec

Dried Blood Spot Autosampler DBSA Materials in the Flow Path • Via GERSTEL MAESTRO software, coupled to the Agilent Mass Hunter®, SCIEX Analyst® or Thermo Scientific™ Xcalibur™ software or stand-alone Storage Conditions*' • Relative humidity 85%, non-condensing • Only one sequence table for the complete system, even • USB connection for camera control, to connect to a Communication Ports*' Power Supply*' Protection Types*' Operating Conditions*' • Relative humidity 20-80%, non-condensing This autosampler is for research use only, it is *> This data only applies to the DBS module without the MPS....