SEPARATION TECHNOLOGY FOR ALTERNATIVE PROTEINS PRODUCED BY FERMENTATION Revolutionize fermentation with our advanced separation solutions Engineered For Your Success

CONTENTS Alternative proteins revolutionized Page 02 Making it big - even with microorganisms Page 04 Process overview: Flottweg technology for the separation of alternative proteins Page 05 Separation of alternative proteins from algae Page 06 Separation of alternative proteins from mycelium Page 07 Fermentation-based protein separation with Sedicanter® Page 08 Harvesting process of alternative proteins from yeast Page 09 Special features of Flottweg decanters and Sedicanters® Page 10 Flottweg separators Page 12 Fully automated value creation Page 14 Centrifuge cleaning Page 15 Flottweg quality...

PROTEINS REVOLUTIONIZED: EFFICIENT RECOVERY AND SUSTAINABLE USE In recent years, alternative proteins have emerged as a key factor in meeting the growing global demand for sustainable food and as effective drug in the pharmaceutical industry. However, the production process of these proteins presents unique challenges to manufacturers. From scaling up production processes to ensuring product purity and consistency, overcoming these challenges requires advanced technologies. Issues such as efficient recovery of cellular biomass, compliance with strict regulations and ensuring high product quality...

Flottweg supports the biotechnology industry in meeting its challenges by providing following services: • High efficiency: Quick and efficient separation processes to maximize yield • Scalability: Flexible solutions for various production scales • Consistency: Reliable operation ensuring consistent product quality • Cost-effective: Reduced operational costs through energy-efficient designs • New market opportunities: Flexible use of the machines, e.g. through recipe control technology tailored to the customer process Flottweg is certified to DIN EN ISO 9001:2015 and manufactures its products...

MAKING IT BIG EVEN WITH MICROORGANISMS Flottweg has positioned itself as a leading supplier of innovative separation technologies and customized solutions for biotechnological applications. Our products e.g. the nozzle separator and the Sedicanter® are known for their efficiency and reliability. Flottweg machines are characterized by maximum performance, outstanding service and adaption to individual applications to optimize your production process. Thanks to the products developed specifically for biotechnology, we are able to provide our customers with the best possible advice and offer customized...

Process overview: Flottweg technology for the separation of alternative proteins Micro-, macroalgae Cultured meat, cultivation products Funghal biomass, yeast Flottweg solution Flottweg solution Flottweg solution eparators and Sedicanter® for S biomass separation. eparators and Sedicanters® S for biomass separation and/ or clarification to recover products in the liquid phase Biomass Ingredients, raw materials for novel food Biomass Protein, fat and other nutrient compounds Biomass Proteins, fat and other fermentation products ® = registered trademark in different countries. ecanter, Sedicanter®...

SEPARATION SEPARATION OF OF ALTERNATIVE ALTERNATIVE PROTEINS PROTEINS E.G. E.G. FROM FROM ALGAE ALGAE Flottweg separators and Sedicanters® are being used for concentrating, extracting and washing algae. Both centrifuge types are suitable for plants producing algae in open ponds as well for closed systems e.g. bioreactors. Corrosion-resistant special steels are optionally available to meet the requirements of saltwater algae. Algae concentrate Algae suspension Fermenter/ photo bioreactor Flottweg Disc Stack Centrifuges Tank for algae concentrate ® = registered trademark in different countries....

DEWATERING DEWATERING OF OF ALTERNATIVE ALTERNATIVE PROTEINS PROTEINS E.G E.G MYCELIUM MYCELIUM Flottweg belt presses are ideally suited for the gentle yet effective dewatering of mycelium biomass. Their controlled pressing mechanism removes moisture efficiently while preserving the structural integrity of the biomass – a key advantage for downstream processing and product quality. Depending on the solid content and structure, the belt press can be used directly after fermentation or following a pre-thickening step. The system’s high adaptability allows for precise adjustment to different solid...

FERMENTATIONFERMENTATIONBASED BASED PROTEIN PROTEIN SEPARATION SEPARATION WITH WITH SEDICANTER® SEDICANTER® The Flottweg Sedicanter® is used when solids and the sediment to be processed are too soft and fine-grained for the classic decanter. ® = registered trademark in different countries. Clarified liquid Its areas of application therefore include so-called “soft products” such as biomasses, yeast and protein suspensions as well as fermentation products. Flottweg Sedicanter® Solid Option Clarified liquid Dilation/ washing Flottweg Sedicanter® Solid Production of mycelium as meat replacement...

HARVESTING HARVESTING PROCESS PROCESS OF OF ALTERALTERNATIVE NATIVE PROTEINS PROTEINS E.G. E.G. FROM FROM YEAST YEAST Alternative proteins can also be harvested as inclusion bodies of microorganisms (e.g. silk protein, yeast protein). In this case, the main product must be extracted from inside the cells. The first step is to separate the whole cells from the fermentation media, followed by cell lysis (mechanical, chemical, enzymatic). Subsequent washing and concentrating steps are taken for further inclusion body purification. Depending on the solids concentration and the required clarification...

SPECIAL SPECIAL FEATURES FEATURES OF OF FLOTTWEG FLOTTWEG DECANTERS DECANTERS AND AND SEDICANTERS® SEDICANTERS® Flottweg decanter Flottweg decanter centrifuges used in precision fermentation processes are hygienically optimized in accordance with EHEDG guidelines and fully Clean-in-Place (CIP)-capable. Special CIP nozzles ensure effective cleaning of critical areas. ll parts in contact with the product are made of high-quality A stainless steel with appropriate surface treatment. Seals are available in FDA-approved material. The gearboxes are installed outside the machine. Liquid discharge Solids...