Clarification for Mammanlian Cells and mAB's

Product catalog summary

Clarification of Mammalian Cells & mAb’s

Background: The production of biological products like monoclonal antibodies (mAb) often involves mammalian cells. After expression in a bioreactor, the suspension must be clarified to remove cells and impurities. Traditional clarification involves primary and secondary steps, often using centrifugation and depth filtration.

Challenge: Higher cell densities and mAb yields increase biomass, complicating the clarification process. Traditional methods are time-consuming, especially for small-scale productions.

Solution: FILTROX offers both traditional and single-use clarification solutions. The single-use approach, PURADISC® BIO SD, combines depth and alluvial filtration, eliminating centrifugation and reducing the process to one step.

Single-use Approach: The PURADISC® BIO SD system is scalable and adaptable, using diatomaceous earth (DE) as a filter aid to enhance filterability. It supports efficient clarification from lab to production scale.

Traditional Approach: FILTROX provides depth filtration systems with large filter areas in compact designs. The systems are scalable and offer various PURAFIX® grades for different filtration needs.

Product Descriptions:

PURACAP® BIO SD: Capsules for lab trials and small batches.
PURADISC® BIO SD: Modules for pilot and production scale.
CELPURE®: Pharma-grade diatomaceous earth to prevent filter clogging.
DISCSTAR® BIO SD: Stainless steel housing for filter module protection.

Optimization and Scalability: The systems allow for flexible optimization with different filter aids and are linearly scalable from development to large-scale production.

Conclusion: FILTROX provides comprehensive solutions for the efficient and scalable clarification of mammalian cells and mAb, addressing industry needs for more effective methods.

Catalog excerpts

Clarification for Mammanlian Cells and mAB's-1

MAMMALIAN CELLS & mAb‘s DEPTH FILTRATION FOR VALUABLE LIQUIDS. SINCE 1938.

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Clarification for Mammanlian Cells and mAB's-2

Table of contents Clarification of Mammalian Cells & mAb‘s: Background, Challenge, Solution page 3 Single-use approach of clarification page 5 Traditional approach of clarification page 7

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Clarification for Mammanlian Cells and mAB's-3

Clarification of Mammalian Cells & mAb’s The Background Biological products such as monoclonal antibodies (mAb), hormones or enzymes are often produced by means of mammalian cells (e. g. CHO cell culture). Nowadays, mammalian cell culture is a key technology in many biotechnological processes. After the expression of the desired product in a bioreactor, the suspension is clarified in order to remove cells, cell debris, and impurities. For the clarification/harvest step, different approaches are possible. The typical clarification process involves a primary and a secondary clarification step....

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Clarification for Mammanlian Cells and mAB's-4

PURACAP® BIO SD capsules: size 2", 5" and 10"

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Clarification for Mammanlian Cells and mAB's-5

FILTROX Single-use Clarification Approach Small scale Buffer tank figure 1 PURADISC® BIO SD filter module 2" for lab trials, 5" for batches up to 10 L, 10" for batches up to 25 L Adaptable to user needs Removal of cells and cell debris 12M, 12S, 12D and 16D depth filter modules in a bag with space to build a filter cake Adaptable to user needs Removal of cells and cell debris in pilot and production scale 30 g to 15 kg pharma grade diatomaceous earth (DE) Pharma grade filter aid Avoids clogging of the filter, increases the filterability up to 4 times Stainless steel support housing for PURADISC®...

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Clarification for Mammanlian Cells and mAB's-6

FILTROX depth Filtration in the Traditional clarification approach Large filter area on a small footprint Depth filter modules and filter sheets allow large filter areas to be handled easily thanks to their compact design. Flexible Optimization Various PURAFIX® grades are available, from coarse to sterile filtration. This enables you to find the right solution for your process. Linearly Scalable Depth filtration technology is easily scalable due to its linearity. The filter material is identical from lab scale to production scale. FILTROX offers depth filtration systems for any size of operation....

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Clarification for Mammanlian Cells and mAB's-7

Small/medium scale Buffer tank PURADISC® filter module DISCSTAR® LS PURADISC® filter module Buffer tank PURADISC® filter module Buffer tank PURAFIX® filter sheets NOVOX® CP PURAFIX® filter sheets Buffer tank PURAFIX® filter sheets Low ion and low pyrogen containing filter sheets Sizes between 6 cm round and 1.2 × 2.4 m possible Clarification of high value liquids Filter modules in 12" and 16" with PURAFIX® sheets Clarification of high value liquids Housing for filter modules For 1 to 4 modules (with up to 16 lenses per module) Depth filtration of turbid solutions Horizontal housing for filter...

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Clarification for Mammanlian Cells and mAB's-8

Filter Presses and Module Housings Filter modules are mounted within a enclosed housing DISCSTAR®, which minimizes oxygen uptake during filtration. Flat sheets are used in open systems like the NOVOX® series. DISCSTAR® BIO SD 12" double support system DISCSTAR® H horizontal housing for filter modules DISCSTAR® LS vertical housing for filter modules NOVOX® CP sheet filter

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Clarification for Mammanlian Cells and mAB's

Clarification for Mammanlian Cells and mAB's

Catalog excerpts

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