pco.flim brochure
10Pages

flim camera system fluorescence (luminescence) lifetime imaging application simplified frequency synthesizer 5 kHz - 40 MHz high frame rate 45 double frames/s lifetimes from 100 ps to 100 µs unique resolution 1008 x 1008 pixels

FLUORESCENCE LIFETIME IMAGING – APPLICATION SIMPLIFIED… For many years the phenomenon of photoluminescence has been used for a variety of purposes in life science applications, ranging from bio markers to sensing applications. Each of the luminophores that are applied has multiple characteristic parameters, which can be exploited for investigations. The most prominent parameter is the luminescent emission itself, the fluorescence or phosphorescence intensity. The intensity is used qualitatively and quantitatively, but the latter strongly depends on the light field and the optical conditions...

FLUORESCENCE LIFETIME IMAGING – APPLICATION SIMPLIFIED… Therefore a phase angle offset of ΔΦ = 90° is introduced between the excitation and the emission, and the measurement is repeated (lower signal in figure 2). Now the integrals correspond to the phase angles of 90° (tap A) and 270° (tap B). With these values or images it is possible to calculate three images: an image of the luminescence intensity, an image of the phase angle distribution and an image of the modulation index distribution. The latter two can be converted into luminescence lifetime distributions. Since one integration in...

FLUORESCENCE LIFETIME IMAGING – APPLICATION SIMPLIFIED… Frequency vs. time domain luminescence lifetime measurements In theory there is no difference regarding the information content of the two types of decay or lifetime measurements, since both methods give the same results, but with different experimental requirements. The frequency domain measurement requires a reference measurement to cancel out the influence of the optical path, which might not be necessary for the time domain measurement, but time domain measurements with image sensors are not possible down to the nanosecond range,...

FLUORESCENCE LIFETIME IMAGING – APPLICATION SIMPLIFIED… pco.flim camera system The pco.flim camera system, consisting of pco.flim camera and pco.flim laser, includes a complete frequency synthesizer, which is required for the generation of the modulation signals in the frequency domain. Application simplified Integrated into an optimized software environment the measurement of 2D fluorescence lifetime distributions now has been simplified. Instead of an image intensifier camera, light source and timing or frequency generators, the application requires only a CMOS camera and a light The...

FLUORESCENCE LIFETIME IMAGING – APPLICATION SIMPLIFIED… Applications Seemlesly integrated into Nikon NIS Elements AR software to use the pco.flim camera for homodyne frequency domain fluorescence lifetime imaging with all referencing and phasor plot feedback. Endogenous fluorescence of a Convallaria (lily of the valley) slice sample. The image shows the endogenous fluorescence lifetime distribution derived from the measured phase angle in false color coding and weighted by the fluorescence intensity. The displayed lifetimes range from 0.5 - 4 ns. HEK-293 cells co-expressing a fusion protein...

FLUORESCENCE LIFETIME IMAGING – APPLICATION SIMPLIFIED… Technical Data Camera System The pco.flim camera system is the first luminescence lifetime imaging camera using a new modulatable CMOS image sensor. It offers all the required generation of frequency domain signals (5 kHz – 40 MHz) and also allows the use of external modulation signals in a limited frequency range (500 kHz – 40 MHz). image sensor type of sensor image sensor resolution (h x v) pixel size (h x v) sensor format / diagonal shutter mode fullwell capacity readout noise dynamic range quantum efficiency spectral range dark...

FLUORESCENCE LIFETIME IMAGING – APPLICATION SIMPLIFIED… pco.flim laser The pco.flim laser is an excitation and illumination light source designed for use with the FD-FLIM pco.flim camera system. It features a digital (square wave) modulation frequency range of 0 – 250 MHz, which ideally covers the modulation frequency range of 5 kHz – 40 MHz offered by the pco.flim camera. There is a full range of different wavelengths available (375 nm – 395 nm – 405 nm – 445 nm – 473 nm – 488 nm – 515 nm – 638 nm). The system is optimum suited for the measurement of fluorescence lifetimes in the range of...

Technical Data Laser wavelengths & powers (selectable; other wavelengths and powers on request) laser safety classification 375 nm / 70 mW 395 nm / 120 mW 405 nm / 120 mW 445 nm / 100 mW 473 nm / 100 mW 488 nm / 100 mW 515 nm / 100 mW 638 nm / 150 mW • 3 mm liquid light guide • FC/APC • FC/PC • FCP8 • 0.7 mm collimated beam long term power stability < 0.5 % / 8 h analogue modulation > 3 MHz (0.5 V @ 1.2 kOhm input signal type or 0..1 V @ 50 Ohm - user configurable) input signal type laser enable (electronic shutter) > 500 kHz input signal type (full ON/OFF) (LV-TTL @ 2 kOhm) opto-mechanical...

europe PCO AG Donaupark 11 93309 Kelheim, Germany +49 9441 2005 50 info@pco.de pco.de america PCO-TECH Inc. 6930 Metroplex Drive Romulus, Michigan 48174, USA +1 248 276 8820 info@pco-tech.com pco-tech.com asia PCO Imaging Asia Pte. 3 Temasek Ave Centennial Tower, Level 34 Singapore, 039190 +65 6549 7054 info@pco-imaging.com pco-imaging.com china Suzhou PCO Imaging Technology Co., Ltd. Room A10, 4th Floor, Building 4 Ascendas Xinsu Square, No. 5 Xinghan Street Suzhou Industrial Park, China 215021 +86 512 67634643 info@pco.cn pco.cn for application stories please visit our website subject to...