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Serum & Cell Culture Reagents

Serum & Cell Culture Reagents
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Serum & Cell Culture Reagents

Product catalog summary
Overview
EuroClone - BioAir is a prominent European manufacturer specializing in microbiological safety cabinets, laminar flow cabinets, and recirculating fume cupboards. With over 35 years of experience, they offer 14 Class II Biological Safety Cabinets compliant with the EN12469 European Standard, catering to diverse quality and budget needs. Their product line also includes laminar flow cabinets for non-toxic samples, recirculating fume hoods, and equipment for automation and industrial applications.
Italian Quality
All products are manufactured in Italy using components of Italian or European origin, ensuring high quality.
Sera and Media
  • Sera: Includes Special Fetal Bovine Sera, Standard Fetal Bovine Sera, and other sera from various species, collected under sterile conditions and tested for contaminants.
  • Standard Media: Offers a variety of media such as Dulbecco’s Modified, RPMI 1640, and others for cell culture.
  • Stem Cell Media, Salts Solutions, Reagents, and Supplements: Includes cell dissociation reagents, amino acids, vitamins, antibiotics, and mycoplasma detection and prevention solutions.
Special Fetal Bovine Sera
Designed for specific applications like Tet-on/Tet-off systems and embryonic stem cell maintenance, rigorously tested for contaminants and performance.
Heat Inactivation and Gamma Irradiation
Heat inactivation is used to inactivate complement proteins, while gamma irradiation reduces or eliminates viruses and bacteria, making the sera suitable for sensitive applications.
Dialysed FBS
Produced by filtration to remove hormones and reduce nucleotides and amino acids, suitable for hormone-free cell culture and sensitive applications.
Other Sera
Includes OptiClone, a cost-effective alternative to FBS with low batch-to-batch variability, and sera from other species like horse, chicken, goat, and rabbit, all collected and processed under strict quality controls.
Human Serum
Obtained from whole blood without anticoagulants, rigorously screened for viruses, and suitable for sensitive cell cultures, especially human cells.
Liquid Media Production
EuroClone specializes in liquid media production using advanced filtration and aseptic-fill technologies, adhering to cGMP guidelines. Products are available in lot sizes up to 5000 liters and packaged in PETG plastic bottles.
Raw Materials
Chemicals used are of the highest purity, conforming to ACS, EU, or USP standards. Water meets USP criteria for Water For Injection (WFI) and is processed through ultrafiltration, reverse osmosis, deionization, and distillation.
Manufacturing Methods and Validation
Manufacturing follows cGMP guidelines, with products sterilized and dispensed in a Class 100 area. Processes are validated for consistency and suitability, intended for research applications only.
Biological Performance
Products are tested for their ability to promote cell growth using various cell lines, including growth promotion, plating efficiency, and cloning efficiency.
Microbiological and Physiochemical Testing
Sterility is tested via membrane filtration, and endotoxin levels are measured using a chromokinetic test. Solubility, pH, and osmolarity are also assessed.
Standard Media
Various media formulations are available, including Dulbecco’s Modified Eagle’s Medium (DME) in low and high glucose variants, Dulbecco’s MEM/F-12, Ham’s Nutrient Mixtures, Minimum Essential Medium (MEM), Medium 199, RPMI 1640, Iscove’s Modified Dulbecco’s Medium (IMDM), and Mc Coy’s 5A Medium, each designed for specific cell culture applications.
Stem Cell Media
EuroMed offers specialized media for mesenchymal stem cells, including serum-free media and differentiation kits for chondrogenic, adipogenic, and osteogenic pathways. Additional products include EuroMed CSC Spheres for tumor cell spheroid formation and EuroMed Genesis Beta Cells Serum Free Kit for pancreatic islet cell differentiation.
Additional Products
EuroMed-N is a basal medium for neuronal precursor cells, and EuroMed-mES is optimized for murine ES cells. EuroMed-mLIF is a cytokine used to maintain undifferentiated ES cells.
Cryoprotective Medium Overview
The document describes a cryoprotective medium, specifically the Freezing Medium II, designed for the cryogenic preservation of standard cell lines. This medium contains DMSO to prevent ice formation during freezing and FBS to reduce dehydration and enhance cell viability post-thawing. It is a ready-to-use solution suitable for storing various cell types in liquid nitrogen.
Product Specifications
The product, identified by Cat.No. ECM0628N, is marketed as EUROMED Freezing II Medium, containing 20% FBS and 10% DMSO, available in a liquid-frozen form.
Primo® Cell Culture Consumables
Primo® Cell Culture products are developed by EuroClone to meet the needs of demanding scientists. These products are manufactured in a clean-room environment under an ISO 9001-2008 Quality System. They are made from high-quality materials like USP VI crystal class virgin polystyrene and polyethylene, ensuring optimal cell growth and attachment.
Primo® Flasks and Plates
These are vacuum-plasma treated to create a negatively charged, hydrophilic surface, promoting consistent cell attachment and growth. The screening plates are designed for high content screening and assays requiring high signal-to-noise ratios.
Primo® Mechanical Pipettes and Tips
Primo® pipettes offer precision and reproducibility, are fully autoclavable, UV-resistant, and come with a 3-year warranty. The tips are compatible with most pipettes, DNase/RNase certified, and free from DNA and pyrogens. They are made from medical-grade virgin polypropylene, minimizing sample retention and biomolecular binding.
Manufacturing and Quality Assurance
The manufacturing facility is fully automated, operating in a controlled clean-room environment. EuroClone SpA maintains a Quality System certified under UNI EN ISO 9001:2008 and NF EN ISO 13485:2004 standards.
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Catalog excerpts

Serum & Cell Culture Reagents-1

serving science through innovation Serum & Cell Culture Reagents

 Open the catalog to page 1
Serum & Cell Culture Reagents-2

EuroClone - BioAir is one of Europe’s leading manufacturers and suppliers of highest quality Microbiological Safety Cabinets, Laminar Flow Cabinets and Recirculating Fume Cupboards, with more than 35 years of experience in the field! Our range of 14 different models of Class II Biological Safety Cabinets realized according to the EN12469 European Standard, meets any quality or budget requirement! The product range is completed by Laminar Flow Cabinets for manipulation of non toxic samples, Recirculating Fume Hoods for easy managing of chemicals and volatiles and a series of equipments designed...

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Serum & Cell Culture Reagents-3

PAG.1 SERA Special Fetal Bovine Sera Standard Fetal Bovine Sera Other Sera Dulbecco's Modified Dulbecco's MEM/F-12 Hams Nutrient Mixture Minimum Essential Medium (MEM) Medium 199 RPMI 1640 Iscove's Modified Dulbecco Mc Coy's PAG.14 STEM CELL MEDIA Cell Dissociation Reagents Aminoacids & Vitamins Reagents Antibiotics & Selection Agents Mycoplasma Detection & Prevention Freezing Medium

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Serum & Cell Culture Reagents-5

SERA The raw material is collected in closed sterile bags to avoid bacterial contamination and high level of endotoxins. Serum is transported and stored at -20 °C. The raw serum is collected from EU and USDA approved areas. The quality of our sera is checked at each step of the production process; the filtration and dispensing are perfomed under positive pressure in HEPA-filtered environmentally controlled rooms. To ensure homogeneity serum is “true-pooled”: it is first filtered through a series of three 0.1 μm pore-size filters and then pooled. Sera are all tested for the absence of aerobic...

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Tetracycline Screened FBS Tetracycline Screened FBS is designed for expression studies in Tet-on/Tet-off systems and transfection. The serum is tested for the presence of Chlortetracycline, Oxytetracycline and Tetracycline by a liquid chromatography electrospray ionisation tandem mass spectrometry method. The detection limit is < 0.05 mg/l. • EU approved 100 nm filtered • Batches with undetectable level of any tetracycline derivates • Ideal for researchers using TETon/TEToff systems • Suitable for transfection and expression studies • High growth capacity • Low IgG content

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Serum & Cell Culture Reagents-7

Heat Inactivated FBS FBS Heat Inactivation is performed at 56°C for 30 minutes with consequent inactivation of complement system. The use of Heat Inactivated FBS is suggested when immunology tested are performed. This treatment allows also to inactivate viruses and to destroy some bacterial contaminants such as mycoplasma. • Heat inactivation treatment is carried out in a controlled environment to reproduce a consistently accurate procedure • Heat Inactivation performed at 56°C for 30 mins • EU approved: Origin depends on batch number (mainly Latin America) • USDA approved: Origin depends on...

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Serum & Cell Culture Reagents-8

Special Fetal Bovine Sera Dialysed FBS Dialysed FBS is produced by a filtration process that removes many hormones and reduces the content of nucleotides and amino acids. The cut-off is 10KDa. The serum is dialysed against a solution of sterile PBS. The use of Dialysed FBS is suggested for hormone free cell culture, minimal essential growth supplement and incorporation studies. • This treatment removes some growth factors • Mainly used for special applications • 100 nm filtered Ultra-low Edotoxin Each manufactured batch is rigorously controlled, from the collection of serum and throughout all...

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Serum & Cell Culture Reagents-9

EuroClone Standard Fetal Bovine Sera are characterized by high quality, sterility and homogeneity; they are used as the main source for growth factors, vitamins, hormones, attachment and transport factors and other cell stimulating components. The raw sera are collected from EU and USDA approved areas. EuroClone preselects the lots and offer its customers only the best performers one. ■ EU approved: Origin depends on batch number (mainly Latin America) ■ USDA approved: Origin depends on batch number (mainly Mexico origin) ■ 100 nm filtered

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Serum & Cell Culture Reagents-10

Other Sera OptiClone Fetal Bovine Serum is a natural, organic product which can vary significantly in its biochemical composition between batches. The results of research depend sometimes on the batch of FBS used. With OptiClone, costumer can work under reproducible conditions due to the low batch to batch variability. OptiClone Serum has been carefully selected and extensively tested to guarantee performances and consistency. It has been demonstrated to promote the cell growth in more than the 80% of the tested cells. OptiClone is suitable for all applications where standard FBS is used. OptiClone...

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Serum & Cell Culture Reagents-11

Human Serum Human Serum off the clot is obtained from whole blood which has not been treated with anticoagulants. It goes through the natural blood clotting process and only then it is centrifuged, removing the solid constituents of the blood together with the fibrin. It contains neither anti-A nor anti-B antibodies. Each batch is rigorously controlled and screened for Hepatitis B (HBS), Hepatitis C (HCV) and HIV Type 1 and 2 (HIV1/2). The use is suggested for sensitive cell culture, it is suitable for most human cells and it is specially recommended for lymphocytes and human macrophages. The...

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Serum & Cell Culture Reagents-12

STANDARD MEDIA EuroClone utilizes its state-of-the-art filtration and aseptic-fill technologies to manufacture its line of liquid media. All facilities and processes are thoroughly validated to ensure that our products meet quality standards and relevant cGMP guidelines. Standard formulas are available in lot sizes up to 5000 litres. All liquid products are manufactured using Water For Injection (WFI) Quality Water. EuroClone liquid media are packaged in inert polyethylene (PETG) plastic bottles. Manufacturing Information Raw Materials Chemicals used to manufacture EuroClone liquid media, salt...

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Serum & Cell Culture Reagents-13

Dulbecco’s Modified Eagle’s Medium, High Glucose (DME/HIGH) Dulbecco's Modified Eagle's Medium, High Glucose (DME/HIGH) contains a four-fold increase in the concentration of amino acids and vitamins found in Eagle's Basal Medium (BME). DME media were originally developed for use with a serum supplement in a 10% CO2 atmosphere for the culture of non-transformed mouse and chicken cells. DMEM and its modifications are widely used to support the growth of a broad spectrum of mammalian cells. Dulbecco’s Modified Eagle’s Medium, Low Glucose (DME/LOW) Dulbecco's Modified Eagle's Medium, Low Glucose...

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*Prices are pre-tax. They exclude delivery charges and customs duties and do not include additional charges for installation or activation options. Prices are indicative only and may vary by country, with changes to the cost of raw materials and exchange rates.