Syringe Mounted for Full Automation automation for every laboratory

µSPEed® are syringe mounted high resolution cartridges for sample preparation Designed for SPE automation, µSPEed has a one-way valve and push together high-pressure seal µSPEed Advantage Efficient and Powerful 1000:1 concentration factor can be achieved in minutes, allowing high resolution trap and elute without solvent blowdown Clean Samples Small particle sorbents used in µSPEed enable ultra-clean preparation of even the dirtiest samples. Raw blood can be put through the cartridges Improved Reproducibility Controlled sample loading and elution results in highly reproducible preparation using...

µSPEed vs. other SPE Methods is faster than conventional SPE because smaller particle size sorbents are used in µSPEed. It performs like a high resolution HPLC column with elution in a narrow precise band. Resolution of targeted compounds in conventional SPE is very limited due to large particle size and inefficient packing of these sorbents. Smaller particle size sorbent gives greater efficiency of extraction, with elution conditions becoming far less critical. Also, the kinetics of the extraction is less critical with the greater efficiency. Where conventional large particle SPE requires precise...

Typical Operation An Example of How µSPE Works STEP 1: [ACTIVATION] Aspirate and Dispense Conditioning Solvent (min 50µL) STEP 2: [CONDITIONING] Aspirate and Dispense (100µL) Conditioning Solvent (min 50µL) STEP 3: [SAMPLE LOAD] Aspirate Sample to Trap Analyte(s) STEP 4: [SAMPLE DISPENSE] Dispense Sample and Needle Wash to Trap Analyte(s) STEP 5: [WASH] Aspirate and Dispense Wash (typically same as Conditioning) Solvent STEP 6: [ELUTION] Aspirate and Dispense Elution Solvent(s) Powerful Syringe Connection µSPEed is excellent for automation, featuring a high pressure, low dead volume connection...

Customisable Chemistry Cartridges Versatile as it is, µSPEed has a wide range of applications. In the field of biochemistry, µSPEed Cartridges have proven to be a highly efficient tool for purifying protein samples. µSPEed-Cxyl customisable cartridge allows a range of customer selected ligands to be simply and quickly bonded to the µSPEed cartridge in-situ. This simple and highly selective modification with a selective ligand, such as an antibody or trypsin reactor, permits targeted and very efficient analysis for specific compounds or biological molecules. In addition, the attachment of enzymes...

µSPEed Example Applications µSPEed Direct Analysis of Caffeine in Saliva Water Disinfection By-Product (DBP) Concentration Instant Coffee Caffeine Std Caffeine in saliva Direct µSPEed Cartridge to UV analysis of caffeine µSPEed DBP concentration from water Fast microExtraction of Polyphenols in Teas PFAS Concentration by Automated µSPEed Linear µSPEed extraction recovery over 10-fold concentration µSPEed concentration of PFBA (1), PFPeA (2), PFBS (3), PFHxA (4), PFHpA (5), PFHxS (6), PFOA (7), PFNA (8), PFOS (9), PFDA (10), PFUnA (11), PFDoA (12), PFTeDA (13). microExtraction of Drugs from Serum...

Direct pSPEed-MS - Opiate Standards Field Sample Collection Using pSPEed Cartridges Herbicide Residue in Roadside Drain extraction/cleanup Sample Preparation without Surrogate Standard“Dirty” Plasma and Urine Extraction - SPEmx microSPE of Calibration Standards and Samples without the need for surrogate standards (pSPEed/ePrep) Local anesthetics spiked into rat plasma samples and then extracted using SPEmx C18 cartridge Note: Please visit our website www.eprep-analytical.com for additional information on these and other applications

Ordering Information Part No Ordering Information Part No Code Sorbent Description automation for every laboratory ePrep Pty Ltd 3 Kingston Town Close Oakleigh, VIC, 3166 AUSTRALIA ePrep® is a registered trademark of ePrep Pty Ltd pSPEed® is a registered trademark of ePrep Pty Ltd eZy-Connect™ is a registered trademark of ePrep Pty Ltd Other trademarks are trademarks of their respective owners Publication No. 98-15001-04 Jan 2022 © Copyright 2022, ePrep Pty Ltd