OVERVIEW AND SOFTWARE Single photon counting sensitivity, optical design, reliability and unrivalled Fluoracle® software. MEASUREMENT EXAMPLES Various types of measurements, scans and sample types. The FS5 is a fully integrated, purpose-built spectrofluorometer. Suited for analytical and research laboratories, the FS5 can handle the speed of routine analysis and the sensitivity of demanding research requirements. Comprehensive Fluoracle® software allows for astonishing ease of use and the design concept enables maximum flexibility, with multiple measurement modes all in one instrument: > Steady...

Excitation Monochromator Emission Monochromator Light Sources Transmission Detector Reference Detector Emission Detectors Sample Module NIQUE TECH S Quantum Yield Time-Resolved Luminescence Spectra Absorption Spectra Plate Reader FRET TemperatureDependent Fluorescence Fluorescence & Phosphorescence Lifetime Water Quality Materials Research Analytical Science Quantum Dots Biological Tissue Natural & Synthetic Dyes Food & Beverage Environmental Science Lanthanides Quality Control Phosphors Bioscience Drug Discovery Photovoltaic Materials Upconversion Materials

INSTRUMENT SENSITIVITY To achieve optimal sensitivity, resolution, and acquisition speed, we engineer the FS5 with an air-sealed excitation path to enhance the excitation power in the UV, select only the optimal performance photomultipliers in temperature stabilised housings, utilise an optical design that includes plane, spherical, toroidal, ellipsoidal mirrors and optimised mirror coatings. The FS5 is truly unrivalled in its spectral performance and sensitivity. Not only does this superior design guarantee the highest sensitivity and broadest wavelength coverage, it has also been optimised...

FLUORACLE® SOFTWARE The operating software for the FS5 Spectrofluorometer is named Fluoracle. This is an exceptional software package specifically written for instrument control and data handling. Fluoracle controls all FS5 steady state and timeresolved features with a straightforward design concept. It focuses on all modern photoluminescence spectroscopy applications, while at the same time provides a userfriendly interface with ‘ready to publish’ outputs. Whether you select a basic spectral scanning version of the FS5 or a version that includes advanced accessories, the software will provide...

EXCITATION AND EMISSION SCANS In spectral scans, fluorescence intensity is recorded as a function of excitation or emission wavelength. The data is automatically corrected for the spectral response of the instrument and variations in excitation power thanks to its accurate, factory generated correction files and silicon reference detector. The FS5 delivers fully corrected, reliable data without any post-processing steps. Right: Anthracene in cyclohexane, excitation (blue) and emission (red) spectra. MEASUREMENT EXAMPLES SYNCHRONOUS SCANS In synchronous spectral scans, the excitation and emission...

ABSORPTION / TRANSMISSION SCANS The FS5 is equipped with a transmission detector as standard which enables the absorption and fluorescence spectra of a sample to be measured using a single instrument. The transmission detector can also be used to monitor the change in transmission through a sample during a fluorescence measurement and correct for any changes in absorbance that occur; which is particularly useful for unstable biological and photosensitive systems. Right: Absorption and emission spectra of a perovskite quantum dot in cyclohexane. It reveals that the fluorescence emission occurs...

EXCITATION-EMISSION MAPS Excitation-Emission Maps (EEMs) provide a ‘fingerprint’ of complex mixtures of substances. These maps can be measured either by a series of emission scans with a stepwise increase or decrease of the excitation wavelength or by a series of synchronous scans and stepwise increase of the excitation-emission offset. A map measurement over a wide range of excitation and emission wavelengths, as shown here, can only be performed properly if higher order scatter is automatically removed during the measurement. The FS5 utilises filter wheels with order sorting filters to remove...

TEMPERATURE CONTROL There are a range of temperature control modules for the FS5, enabling the change in fluorescence and absorption of samples to be measured over a temperature range of 77K to 500K. An overview of some of the most popular modules for temperature control are highlighted below. Temperature Controlled Cuvette Holder TE Cooled (-50°C to +150°C) Temperature controlled cuvette holders for fluorescence and transmittance measurements of solutions. The temperature is fully controllable through the Fluoracle operating software of the FS5, which allows spectral and lifetime temperature...

The FS5 upgrade options are modifications or additions to the main body of the FS5. All upgrades are best installed at the time of manufacture, but they can also be retro-fitted by a qualified service engineer during an on-site visit. All the standard features of the FS5 are retained when an upgrade option has been added and many upgrade options can be combined. This offers outstanding flexibility for an instrument of this class. WAVELENGTH EXTENSION UPGRADES UV Wavelength Extension Enhance and extend the range of UV excitation with an ozone-generating bulb and an excitation grating optimised...

LIFETIME EXTENSION UPGRADES FS5-MCS: Lifetimes from Microseconds to Seconds 118 μs 444 μs Phosphorescence lifetime measurements in the lower microsecond (>5 μs) to second time range is done by a technique called Multi-Channel Scaling (MCS). This upgrade features a microsecond xenon flashlamp as an additional excitation source. Lifetimes in the range of >5 μs to <10 s can be measured and the software incorporates fitting and reconvolution analysis for lifetime evaluation. Some luminescent samples or mixtures possess overlapping fluorescence and phosphorescence spectra. The FS5-MCS upgrade allows...