• Up to ±3000 V* at 700 μA • Dual channel operation • Monitor current and voltage • Piggyback units for four channels • Serial communication • Trigger external devices The HV Sequencer (ER230) is a dual channel high voltage power supply especially designed for electroosmotic flow (EOF) applications using microfluidic chips. Suitable for microchip electrophoresis with: • C4D Microchip Electrophoresis Platform, ET121 • C4D Micronit Platform, ET122 both of which are supplied with suitable cabling. Also available is a set of four high voltage cables, SHV connector to bare wire termination (EC230), for attachment to other devices. • Microfluidic chip channel electrophoresis • Electrokinetic chromatography • Micro reactors/mixers using EOF pumping The HV Sequencer comprises two independent programmable high voltage supplies, and two metering inputs that can provide a high impedance voltmeter connection or a low impedance current measuring circuit. Sequencer™ control software is supplied for Windows Vista, XP or later computers. In addition, serial communication via a USB port is supported for users wishing to develop their own sequencing software. The HV Sequencer is powered from the 12V DC mains adaptor (supplied). Meter Inputs High voltage outputs Programmable, rear panel, logic outputs can be used to control external devices. Status High Voltage Logic outputs Monitoring of voltage and current are also provided on the high voltage output terminals to quickly identify any problems caused by bubbles and other channel blockages. Feedback control of output voltage provides for accurate and stable operating conditions. Grounding post The system can be expanded by piggybacking a second Sequencer via the I2C back panel connectors. This arrangement provides four synchronised (sub-millisecond timing) high voltage outputs for more complex experimental protocols. The easy-to-use Sequencer™ software (supplied) creates tables of sequences of voltage levels for applying to the various fluidic ports of the microchip. A safety interlock features ensures that the unit can only be armed by conscious action of the user.

Serial Interface Multiple units (or piggybacked pairs of units) can be operated as virtual RS232 devices using a serial protocol over a USB connection with custom written software or using packages such as: • • • • LabVIEW™, www.ni.com Connect™, www.labtronics.com/DI/RS232_Software.htm WinWedge®, www.taltech.com/products/winwedge.html HyperTerminal™, www.microsoft.com Load in OC mode Inject in OC mode Clean in SetVmode Sample Waste To Meter 2, Load in SC mode Inject in SetV mode Clean in SC mode Run in OC mode Buffer waste To Meter 1, Load in OC mode Inject in SC mode Clean in OC mode Run in...