ANALYTICAL AND PREPARATIVE CHROMATOGRAPHY COLUMNS COLUMNS Product Guide

WHAT IS NEW ASTRA® 2 µm HPLC columns New ASTRA® HPLC columns are complementary to our ARION® brand. These columns offer not only a high-endcapped C18 phase, but also a unique DM phase with dual modification. Another novel phase C18-AQ offers dual chemistry bonding for analyses in aqueous mobile phases. See page 54. ARION® BIO and Biphenyl New ARION® SAX and SCX columns extend the HPLC column range into ion-exchanged chromatography. The reversed phases have been extended with the Biphenyl phase to offer a wider range of selectivities. See page 46. ARION® preparative guard cartridges The ARION®...

HPLC COLUMN SELECTION HPLC COLUMN SEL HPLC column selectivity The selectivity is defined as the capability of stationary phases to separate analytes under certain conditions. The selectivity mainly depends on the structure, metal content and modification of base silica gel, type of chemical bonding or silica gel end-capping. The HPLC column selectivity has been measured according to the Tanaka plots (Tanaka, N. et al., Journal of Chromatographic Science, 27 (1989), 721–728), shown below. This allows users a quick comparison of different phases in order to choose the best possible HPLC columns...

As ARION® is one of the latest objects found in space, so it is also the best workhorse for your applications. Explore our new line of ARION® HPLC columns. What innovations does this column bring to you? • Strict quality control of alkaline and metal content during the silica gel production. • Narrow particle size and pore size distribution. • Unique production process ensuring high lot-to-lot reproducibility. • Good stability at higher temperatures. ARION® Silicagel Particle size 5 pm 2.2 pm Mean particle diameter 5.3 ± 0.9 pm 2.5 ± 0.5 pm Proximity to the shape of a sphere 0.96 ± 0.04 0.97...

The 5-micron electron microscope field clearly shows the highest quality of ARION® 5µm particles. Main particle characteristics: zz The closest proximity to a sphere. zz Unique surface smoothness shown in the pictures above. zz Tight particle size distribution. zz No broken particles. zz No presence of clustered particles. zz No “Moon craters or mounta

Particle size distribution Particle size distribution of ARION® 5 µm particles shows a tight profile calculated from ferret figures by SEM. ARION® column hardware: zz Modern column hardware for easy handling in a narrow space. zz UHPLC grade Stainless Steel with an amazingly smooth internal surface. zz Colour coded fittings. Particle size fraction [%] ARION® partic

Batch to batch reproducibility Batch-to-batch reproducibility is shown in the two bar graphs below. The silica batches are strictly controlled and checked for symmetry, and efficiency (number of theoretical plates/meter). 2.0 1.8 Peak symmetry factor Number of theoretical plates Theoretical plates reproducibility Symmetry reproducibility Both the silanol activity and hydrofobicity tests are defined e.g. by the Engelhardt test. The hydrofobicity test is based on calculation of the ratio of retention factors kethylbenzene /ktoluene. The first picture of the Engelhardt test shows a comparison of...

Batch to batch reproducibility 790 Analysis of two batches based on the Engelhardt test. Column Mobile phase Methanol : water 49/51 (v/v) Isocratic elution Flow rate Analysis of two batches based on the Shape selectivity test. Column Mobile phase Methanol : water 79/21 (v/v) Isocratic elution Flow rate

FOOD APPLICATIONS Alcaloids – xanthine derivatives Xanthine alkaloids occur naturally in various plants, such as cocoa, tea and coffee trees. They are commonly used for their effects as mild stimulants. Xanthine alkaloids are monitored in food and drinks, e.g. in chocolate, cocoa powder, and energy drinks. 400 Theobromine and caffeine standard 1400 1200 Energy drink Part number Mobile phase Flow rate

ARION® FOOD Non-nutritive sweeteners Low-calorie sweeteners are commonly used worldwide in the food and drink industry. The list of approved sweeteners varies from country to country. The most common method used to monitor these highly consumed products involves high performance liquid chromatography (HPLC or UHPLC). Standard mixture Flow rate 2.0 ml/min Analytes 1. Acesulfame-K (ACS-K)

FOOD APPLICATIONS Sodium and potassium salts of benzoic acid and sorbic acid are well-known food preservatives. The permitted amount in food is strictly regulated with the level depending on the food group. As an example, European regulation EC 1333/2008 sets the rules on food additives: definitions, conditions of use, labelling and procedures. 450 100 Standard mixture Fruit syrup sample Flow rate Part number Mobile phase Citrate buffer pH 4.1 : ACN : MeOH 70/20/10 (v/v/v) 1. Sodium benzoate 2. Potassium sorbate Preservatives in fats and oils BHA is used as an antioxidant and preservative in...

Organic acids The identification and quantitative analysis of major organic acids in fruits and vegetables is considered very important for the food and beverage industry. Organic acids play a significant role thanks to their influence on flavour, stability and keeping quality. Organic acids are generated during the aerobic oxidation of carbohydrates, proteins and fats in most biological systems. Column ARION® Polar C18, 5.0 pm Flow rate 1.0 ml/min Analytes 1. Formic acid Standard mixture Drink additives This application shows the separation of three groups of compounds in parallel: non-nutritive...

m, ARION FOOD APPLICATIONS Standard on ARION® column Column_ARION® Plus C18, 3 pm_ Mobile phase A: Water Gradient elution Time A (%) B (%) Flow rate 1.0 ml/min Analytes 1. Retinol (Vitamin A) 5. Alpha-Tocopheryl acetate (Vitamin E acetate)

FOOD APPLICATIONS Vitamins A and E Calibration curves of analytes This application has been developed by WESSLING Hunga